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Cytoprotective Effects of the Red Marine Alga Chondrus canaliculatus Against Maneb-Induced Hematotoxicity and Bone Oxidative Damages in Adult Rats

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Abstract

The current study aimed at evaluating the ability of a mineral and antioxidant-rich extract from Chondrus canaliculatus to improve maneb (MB)-induced toxicity in adult rat. The animals were divided into four groups: group 1 used as a control group, group 2 received MB, group 3 received MB + C. canaliculatus extract, and group 4 received only the algal extract. MB, a Mn-containing ethylene-bis-dithiocarbamate fungicide, induced oxidative stress damages, mineral perturbations in the plasma, urine, and bone, and genotoxicity in rats. Hematological analysis revealed in the MB-treated group a disruption in the number of red blood cells, platelets, and white blood cells associated with a striking genotoxicity. Interestingly, a significant increase in malondialdehyde and advanced oxidation protein product levels in erythrocytes and bones were found. On the other hand, an impairment of the antioxidant status in both tissues was occurred. Along, our results revealed that MB injection caused a striking drop and disruption in bone’s mineral rates, especially calcium and phosphorus. These biochemical results were in accordance with the histological and molecular changes. However, co-treatment with C. canaliculatus extract showed, for the first time, that this alga was effective against MB-induced hematotoxicity, genotoxicity, and oxidative stress in the blood and bone and maintained osteomineral metabolism and bone histo-architecture. Such observations might be explained by the strong in vitro antioxidant and antibacterial activities exhibited by the alga, as well as by its high levels in several minerals: calcium, phosphorus, sodium, potassium, magnesium, iron, and zinc.

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Acknowledgements

Unit of Functional Genomics and Plant Physiology. Higher Institute of Biotechnology of Sfax, 3000, Sfax University, Tunisia.

Funding

The present work was supported by DGRST grants (Direction Générale de la Recherche Scientifique et Technique, Tunisie).

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Author notes

  1. Hajer Ben Saad, Intidhar Bkhairia, and Boutheina Cherif contributed equally to this work.

Authors and Affiliations

  1. Laboratory of Pharmacology, Faculty of Medicine, University of Sfax, 3029, Sfax, Tunisia

    Imen Jaballi, Hajer Ben Saad & Ahmed Hakim

  2. Unit of Functional Genomics and Plant Physiology, Higher Institute of Biotechnology of Sfax, University of Sfax, 3000, Sfax, Tunisia

    Imen Jaballi & Ibtissem Ben Amara

  3. Laboratory of Enzyme engineering and Microbiology, National Engineering School in Sfax, University of Sfax, B.P. 1173, 3038, Sfax, Tunisia

    Intidhar Bkhairia

  4. Unit of Biotechnology and Pathology, Higher Institute of Biotechnology of Sfax, University of Sfax, P.O. Box 261, 3038, Sfax, Tunisia

    Boutheina Cherif

  5. Laboratory of Hematology, CHU Habib Bourguiba, University of Sfax, 3029, Sfax, Tunisia

    Choumous Kallel

  6. Laboratory of Anatomopathology, CHU Habib Bourguiba, University of Sfax, 3029, Sfax, Tunisia

    Ons Boudawara

  7. EA 4324 ORPHY, Faculty of Medicine, University of Brest, 29238, Brest Cedex, France

    Michael Droguet

  8. EA 2219 Géoarchitecture, 6 av. V. Le Gorgeu, University of Brest, 29238, Brest Cedex, France

    Christian Magné

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  1. Imen Jaballi
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  2. Hajer Ben Saad
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  10. Ibtissem Ben Amara
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Correspondence to Ibtissem Ben Amara.

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The experimental procedures were carried out according to the general guidelines on the use of living animals in scientific investigations, approved by the Ethical Committee of the Sciences Faculty of Sfax.

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Jaballi, I., Saad, H.B., Bkhairia, I. et al. Cytoprotective Effects of the Red Marine Alga Chondrus canaliculatus Against Maneb-Induced Hematotoxicity and Bone Oxidative Damages in Adult Rats. Biol Trace Elem Res 184, 99–113 (2018). https://doi.org/10.1007/s12011-017-1151-7

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