Biological Trace Element Research

, Volume 183, Issue 1, pp 128–137 | Cite as

Mitigation of Acute Aluminum Toxicity by Sodium Selenite and N-Acetylcysteine in Adult Male Rats

  • Naglaa H. Nour-Eldein
  • El-Sayed A. Hassanin
  • Wael M. El-Sayed
Article

Abstract

The objective of this study is to investigate the toxic effects of aluminum and the potential alleviation of selenite and N-acetylcysteine (NAC) on this toxicity. Acute aluminum toxicity was induced by intraperitoneal (i.p.) injection of AlCl3 (30 mg Al3+/kg) for four consecutive days. Al3+ damaged the synthetic capability and regeneration power of liver cells and induced inflammation. It also damaged the kidney and disturbed the lipid profile enhancing the total cholesterol level and LDL-cholesterol level increasing the risks of atherosclerosis. Al3+ reduced the cellular antioxidant milieu typified by the decrease in reduced glutathione, vitamin E, and four antioxidant enzymes and induced lipid peroxidation (LPO). Selenite at 1 mg Se/kg and NAC at 150 mg/kg injected either simultaneously with or after Al3+ mitigated most of these damaging effects probably by the virtue of scavenging the free radicals, binding aluminum and stimulating its excretion and reducing its bioavailability, bolstering the endogenous antioxidant defense systems, stabilizing the cell membrane, and preventing LPO. The beneficial effects of selenite and NAC against aluminum toxicity were also confirmed by the light and electron histopathology study. There were no significant differences between the two regimens used (protection and therapeutic) in the current study probably due to the short time of exposure, and the abrogation of Al3+ toxicity offered by selenite was better than that provided by NAC on the histopathology level.

Keywords

Antioxidants Lipid profile Hepatotoxicity Histopathology 

Notes

Compliance with Ethical Standards

Conflict of Interest

The authors declare that they have no conflicts of interest.

References

  1. 1.
    Greger JL (1992) Dietary and other sources of aluminium intake. Alum Biol Med 169:26–29Google Scholar
  2. 2.
    Wilhelm M, Passlick J, Busch T, Szydlik M, Ohnesorge FK (1989) Scalp hair as an indicator of aluminium exposure: comparison to bone and plasma. Hum Toxicol 8:5–9CrossRefPubMedGoogle Scholar
  3. 3.
    Beyer LA, Greenberg G, Beck BD (2014) Evaluation of potential exposure to metals in laundered shop towels. Hum Ecol Risk Assess 20:111–136CrossRefPubMedGoogle Scholar
  4. 4.
    Luo H, Liu G, Zhang R, Bai Y, Fu S, Hou Y (2014) Heavy metal recovery combined with H 2 production from artificial acid mine drainage using the microbial electrolysis cell. J Hazard Mater 270:153–159CrossRefPubMedGoogle Scholar
  5. 5.
    Cheng D, Zhu C, Wang C, Xu H, Cao J, Jiang W (2014) Hepatoprotective effects of apple polyphenol extract on aluminum-induced liver oxidative stress in the rat. Can J Physiol Pharmacol 92:109–116CrossRefPubMedGoogle Scholar
  6. 6.
    Gorozhanskaia ÉG, Sviridova SP, Dobrovol’skaia MM, Zybrikhina GN, Kashnia S (2012) Selenium and oxidative stress in cancer patients. Biomed Khim 59:550–562CrossRefGoogle Scholar
  7. 7.
    Anand R, Sharma DR, Verma D, Bhalla A, Gill KD, Singh S (2013) Mitochondrial electron transport chain complexes, catalase and markers of oxidative stress in platelets of patients with severe aluminum phosphide poisoning. Hum Exp Toxicol 32:807–816CrossRefPubMedGoogle Scholar
  8. 8.
    El-Sayed WM, Aboul-Fadl T, Lamb JG, Roberts JC, Franklin MR (2006) Acute effects of novel selenazolidines on murine chemoprotective enzymes. Chem Biol Interact 162:31–42CrossRefPubMedGoogle Scholar
  9. 9.
    Sharma S, Raghuvanshi BS, Shukla S (2014) Toxic effects of lead exposure in rats: involvement of oxidative stress, genotoxic effect, and the beneficial role of N-acetylcysteine supplemented with selenium. J Environ Pathol Toxicol Oncol 33:19–32CrossRefPubMedGoogle Scholar
  10. 10.
    Aruoma OI, Halliwell B, Hoey BM, Butler J (1989) The antioxidant action of N-acetylcysteine: its reaction with hydrogen peroxide, hydroxyl radical, superoxide, and hypochlorous acid. Free Radic Biol Med 6:593–597CrossRefPubMedGoogle Scholar
  11. 11.
    Viezeliene D, Jansen E, Rodovicius H, Kasauskas A, Ivanov L (2011) Protective effect of selenium on aluminium-induced oxidative stress in mouse liver in vivo. Environ Toxicol Pharmacol 31:302–306CrossRefPubMedGoogle Scholar
  12. 12.
    Horst A, Kolberg C, Moraes S, Riffel K, Finamor IA, Belló-Klein A, Pavanato MA, Partata WA (2014) Effect of N-acetylcysteine on the spinal-cord glutathione system and nitric-oxide metabolites in rats with neuropathic pain. Neurosci Lett 569:163–168CrossRefPubMedGoogle Scholar
  13. 13.
    El-Sayed WM, Al-Kahtani MA, Abdel-Moneim AM (2011) Prophylactic and therapeutic effects of taurine against aluminum-induced acute hepatotoxicity in mice. J Hazard Mater 192:880–886CrossRefPubMedGoogle Scholar
  14. 14.
    Yoshioka T, Kawada K, Shimada T, Mori M (1979) Lipid peroxidation in maternal and cord blood and protective mechanism against activated-oxygen toxicity in the blood. Am J Obstet Gynecol 135:372–376CrossRefPubMedGoogle Scholar
  15. 15.
    Beutler E, Kelly BM (1963) The effect of sodium nitrite on red cell GSH. Cell. Mol Life Sci 19:96–97CrossRefGoogle Scholar
  16. 16.
    Aebi H (1984) Catalase in vitro. Methods Enzymol 105:121–126CrossRefPubMedGoogle Scholar
  17. 17.
    Paglia DE, Valentine WN (1967) Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. Transl Res 70:158–169Google Scholar
  18. 18.
    Masayasu M, Hiroshi Y (1979) A simplified assay method of superoxide dismutase activity for clinical use. Clin Chim Acta 92:337–342CrossRefGoogle Scholar
  19. 19.
    Habig WH, Pabst MJ, Fleischner G, Gatmaitan Z, Arias IM, Jakoby WB (1974) The identity of glutathione S-transferase B with ligandin, a major binding protein of liver. PNAS 71:3879–3882CrossRefPubMedPubMedCentralGoogle Scholar
  20. 20.
    Smirnoff N, Cumbes QJ (1989) Hydroxyl radical scavenging activity of compatible solutes. Phytochemistry 28:1057–1060CrossRefGoogle Scholar
  21. 21.
    Blois MS (1958) Antioxidant determinations by the use of a stable free radical. Nature 181:1199–1200CrossRefGoogle Scholar
  22. 22.
    Kumar V, Bal A, Gill KD (2009) Aluminium-induced oxidative DNA damage recognition and cell-cycle disruption in different regions of rat brain. Toxicology 264:137–144CrossRefPubMedGoogle Scholar
  23. 23.
    Periasamy VS, Athinarayanan J, Alshatwi AA (2015) Aluminum oxide nanoparticles alter cell cycle progression through CCND1 and EGR1 gene expression in human mesenchymal stem cells. Biotechnol Appl Biochem 63:320–327CrossRefPubMedGoogle Scholar
  24. 24.
    Kalimeris K, Briassoulis P, Ntzouvani A, Nomikos T, Papaparaskeva K, Politi A, Batistaki C, Kostopanagiotou G (2016) N-acetylcysteine ameliorates liver injury in a rat model of intestinal ischemia reperfusion. J Surg Res 206:263–272CrossRefPubMedGoogle Scholar
  25. 25.
    Nido SA, Shituleni SA, Mengistu BM, Liu Y, Khan AZ, Gan F, Kumbhar S, Huang K (2016) Effects of selenium-enriched probiotics on lipid metabolism, antioxidative status, histopathological lesions, and related gene expression in mice fed a high-fat diet. Biol Trace Elem Res 171:399–409CrossRefPubMedGoogle Scholar
  26. 26.
    El-serougy E, Ahmed HH, Kamal MM, Niazy MH (2015) The neuropeptide adrenomedullin, could it be linked to renal involvement and disease activity in systemic lupus erythematosus? Egypt Rheumatol 37:19–24CrossRefGoogle Scholar
  27. 27.
    Sivakumar S, Sivasubramanian J, Khatiwada CP, Manivannan J, Raja B (2013) Determination of aluminium induced metabolic changes in mice liver: a Fourier transform infrared spectroscopy study, Spectrochim. Acta Part A Mol Biomol Spectrosc 110:241–248CrossRefGoogle Scholar
  28. 28.
    Liu Y, Wang Y, Walsh TR, Yi L, Zhang R, Spencer J, Doi Y, Tian G, Dong B, Huang X (2016) Emergence of plasmid-mediated colistin resistance mechanism MCR-1 in animals and human beings in China: a microbiological and molecular biological study. Lancet Infect Dis 16:161–168CrossRefPubMedGoogle Scholar
  29. 29.
    Block CA, Manning HL (2002) Prevention of acute renal failure in the critically ill. Am J Respir Crit Care Med 165:320–324CrossRefPubMedGoogle Scholar
  30. 30.
    Mailloux RJ, Lemire J, Appanna VD (2011) Hepatic response to aluminum toxicity: dyslipidemia and liver diseases. Exp Cell Res 317:2231–2238CrossRefPubMedGoogle Scholar
  31. 31.
    Menéndez-Carreño M, Ansorena D, Milagro FI, Campión J, Martínez JA, Astiasarán I (2008) Inhibition of serum cholesterol oxidation by dietary vitamin C and selenium intake in high fat fed rats. Lipids 43:383–390CrossRefPubMedGoogle Scholar
  32. 32.
    Machado JT, Iborra RT, Fusco FB, Castilho G, Pinto RS, Machado-Lima A, Nakandakare ER, Seguro AC, Shimizu MH, Catanozi S (2014) N-acetylcysteine prevents endoplasmic reticulum stress elicited in macrophages by serum albumin drawn from chronic kidney disease rats and selectively affects lipid transporters, ABCA-1 and ABCG-1. Atherosclerosis 237:343–352CrossRefPubMedGoogle Scholar
  33. 33.
    Korou LM, Agrogiannis G, Koros C, Kitraki E, Vlachos IS, Tzanetakou I, Karatzas T, Pergialiotis V, Dimitroulis D, Perrea DN (2014) Impact of N-acetylcysteine and sesame oil on lipid metabolism and hypothalamic-pituitary-adrenal axis homeostasis in middle-aged hypercholesterolemic mice. Sci Rep 4:6806–6813CrossRefPubMedPubMedCentralGoogle Scholar
  34. 34.
    Nehru B, Anand P (2005) Oxidative damage following chronic aluminium exposure in adult and pup rat brains. J Trace Elem Med Biol 19:203–208CrossRefPubMedGoogle Scholar
  35. 35.
    Exley C (2004) The pro-oxidant activity of aluminum. Free Radic Biol Med 36:380–387CrossRefPubMedGoogle Scholar
  36. 36.
    Kerksick C, Willoughby D (2005) The antioxidant role of glutathione and N-acetyl-cysteine supplements and exercise-induced oxidative stress. J Int Soc Sports Nutr 2:38–44CrossRefPubMedPubMedCentralGoogle Scholar
  37. 37.
    Abubakar MG, Taylor A, Ferns GAA (2003) Aluminium administration is associated with enhanced hepatic oxidant stress that may be offset by dietary vitamin E in the rat. Int J Exp Pathol 84:49–54CrossRefPubMedPubMedCentralGoogle Scholar
  38. 38.
    Aslanturk A, Uzunhisarcikli M, Kalender S, Demir F (2014) Sodium selenite and vitamin E in preventing mercuric chloride induced renal toxicity in rats. Food Chem Toxicol 70:185–190CrossRefPubMedGoogle Scholar
  39. 39.
    Fernández V, Vargas R, Castillo V, Cádiz N, Bastías D, Román S, Tapia G, Videla LA (2013) Reestablishment of ischemia-reperfusion liver injury by N-acetylcysteine administration prior to a preconditioning iron protocol. Sci World J 2013:1–9CrossRefGoogle Scholar
  40. 40.
    Baatrup E, Ole TU, Nielsen HL, Wilsky K (1989) Mercury-selenium interactions in relation to histochemical staining of mercury in the rat liver. Histochem J 21:89–98CrossRefPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2017

Authors and Affiliations

  1. 1.Egyptian Mineral Resources AuthorityPetroleum MinistryCairoEgypt
  2. 2.Department of BiochemistryNational Institute of NutritionCairoEgypt
  3. 3.Faculty of Science, Department of ZoologyUniversity of Ain ShamsCairoEgypt

Personalised recommendations