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Effects of Fluoride on Surface Structure of Primary Culture Leydig Cells in Mouse


Fluoride (F) is known to induce reproduction toxicity, and the elucidation of its underlying mechanisms is an ongoing research. These findings aim to provide deeper insights into roles of soduim fluoride (NaF) in testis damage, which could contribute to a better understanding of fluoride-induced male reproductive toxicity. The Leydig cells were administrated by 0, 5, 10, and 20 mg/L NaF for 24 h, respectively. Scanning electron microscope was used to identify the change of surface structure in the Leydig cells. The results showed that fluoride exposure of high-dose induced bulged balloon in the membrane of the Leydig cell. We speculated that high doses of NaF inhibited cell proliferation and induced cell apoptosis in Leydig cells. This is the first time that we have reported that fluoride impaired cytomembrane and cytoskeleton of the Leydig cells in vitro treated with different doses of fluoride for 24 h by using a scanning electron microscope. Damage to the cytoskeleton and cytomembrane may be one of the reasons of male reproductive toxicity induced by fluoride.

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We thank Ram Kumar Manthari for the improvement in English syntax and word usage.

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Correspondence to Jianhai Zhang.

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All regulations of the Institutional Animal Care and Use Committee of Shanxi Agricultural University were strictly followed during the experiment to protect the welfare of the animals.

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This research was supported by the National Natural Science Foundation of China (Grant No.31172380).

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The authors declare that they have no conflicts of interest.

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Li, J., Shi, Y., Fan, H. et al. Effects of Fluoride on Surface Structure of Primary Culture Leydig Cells in Mouse. Biol Trace Elem Res 183, 123–127 (2018).

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  • Fluoride
  • Leydig cells
  • Scanning electron microscope
  • Cytomembrane