Skip to main content
Springer Nature Link
Log in

Effects of Excess Dietary Fluoride on Serum Biochemical Indices, Egg Quality, and Concentrations of Fluoride in Soft Organs, Eggs, and Serum of Laying Hens

  • Published:
Biological Trace Element Research Aims and scope Submit manuscript

Abstract

This study was conducted to investigate the effects of excess dietary fluoride (F) on serum biochemical indices, egg quality, and concentrations of F in soft tissues, eggs, and serum of laying hens. Commercial laying hens (n = 576, 51 weeks of age) were randomly allotted to 6 treatments with 6 replicates of 16 birds. The basal diets contained fluorine inclusions at a level of 16 mg/kg, and graded sodium fluoride was added to the basal diet to achieve fluorine inclusions, respectively, at a level of 200, 400, 600, 800, and 1000 mg/kg in the experimental diets. Dietary F levels at 600, 800, and 1000 mg/kg decreased (P < 0.05) albumin height and yolk color, while eggshell strength and eggshell thickness significantly decreased at 800 and 1000 mg/kg, respectively, compared with the control group. Fluoride concentrations in eggshell, albumin, yolk, liver, kidney, ovary, and oviduct responded to dietary F levels positively, and F concentrations in eggshell were the highest. Fluorine concentrations in albumin and yolk increased with the feeding time at the same dietary F levels (P < 0.05). Dietary F level at 400 mg/kg increased serum calcium level and activity of glutamic oxalacetic transaminase (P < 0.05). In conclusion, dietary F levels at 600 mg/kg decreased albumin height and yolk color, while eggshell strength and eggshell thickness significantly decreased at 800 and 1000 mg/kg, respectively. F concentrations in soft tissues, albumin, yolk, and eggshell of layers had a positive correlation with dietary F levels. By disturbing Ca and phosphorus metabolism, dietary F levels affected the formation of eggshell, reducing eggshell strength and eggshell thickness.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Subscribe and save

Springer+ Basic
$34.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or eBook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

Similar content being viewed by others

Explore related subjects

Discover the latest articles and news from researchers in related subjects, suggested using machine learning.

References

  1. WHO (1973) Trace elements in human nutrition: a report of a WHO expert group. Technical series no 532. World Health Organization, Geneva

  2. Kirck KL (1991) Biochemistry of the elemental halogens and inorganic halides. Plenum, New York, pp 19–68

    Book  Google Scholar 

  3. Everett E (2011) Fluoride’s effects on the formation of teeth and bones, and the influence of genetics. J Dent Res 90:552–560

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  4. Perumal E, Paul V, Govindarajan V, Panneerselvam L (2013) A brief review on experimental fluorosis. Toxicol Lett 223:236–251

    Article  CAS  PubMed  Google Scholar 

  5. Sun Z, Niu R, Wang B, Jiao Z, Wang J, Zhang J, Wang S, Wang J (2011) Fluoride-induced apoptosis and gene expression profiling in mice sperm in vivo. Arch Toxicol 85:1441–1452

    Article  CAS  PubMed  Google Scholar 

  6. Ghosh D, Das Sarkar S, Maiti R, Jana D, Das UB (2002) Testicular toxicity in sodium fluoride treated rats: association with oxidative stress. Reprod Toxicol 16:385–390

    Article  CAS  PubMed  Google Scholar 

  7. Mukhopadhyay D, Chattopadhyay A (2014) Induction of oxidative stress and related transcriptional effects of sodium fluoride in female zebrafish liver. Bull Environ Contam Toxicol 93:64–70

    Article  CAS  PubMed  Google Scholar 

  8. Podder S, Chattopadhyay A et al (2010) Histopathology and cell cycle alteration in the spleen of mice from low and high doses of sodium fluoride. Fluoride 43(4):237–245

    CAS  Google Scholar 

  9. Sharma A, Chinoy NJ (1998) Role of free radicals in fluoride-induced toxicity in liver and kidney of mice and its reversal. Fluoride 31:S26

    Google Scholar 

  10. Ranjan R, Ranjan A (2015) Fluoride toxicity in animals. Springer International Publishing, New York City

    Book  Google Scholar 

  11. Griffith-Jones W (1977) Fluorosis in dairy cattle. Vet Rec 100:84–89

    Article  CAS  PubMed  Google Scholar 

  12. Hillman D, Bolenbaugh DL, Convey EM (1979) Hypothyroidism and anaemia related to fluoride in dairy cattle. J Dairy Sci 62:416–423

    Article  CAS  PubMed  Google Scholar 

  13. Singh JL, Swarup D (1995) Clinical observations and diagnosis of fluorosis in dairy cows and buffaloes: case report. Agri Practice 16:25–30

    Google Scholar 

  14. Del Razo LM, Corona JC, Garcia-Vargas G, Albores A, Cebrian ME (1993) Fluoride levels in well-water from a chronic arsenicism area of northern Mexico. Environ Pollut 80:91–94

    Article  CAS  PubMed  Google Scholar 

  15. Bouaziz H, Croute F, Boudawara T, Soleilhavoup JP, Zeghal N (2007) Oxidative stress induced by fluoride in adult mice and their suckling pups. Exp Toxicol Pathol 58(5):339–349

    Article  CAS  PubMed  Google Scholar 

  16. Monsour PA, Kruger BJ (1985) Effect of fluoride on soft tissues in vertebrates. Fluoride 18:53–61

    CAS  Google Scholar 

  17. Yuan C, Song HH, Zhang XY, Jiang YJ, Zhang AT, Azzam MM, Zou XT (2014) Effect of expanded cottonseed meal on laying performance, egg quality, concentrations of free gossypol in tissue, serum and egg of laying hens. Anim Sci J 85(5):549–554

    Article  CAS  PubMed  Google Scholar 

  18. Pattee OH, Wiemeyer SN, Swineford DM (1988) Effects of dietary fluoride on reproduction in eastern screech-owls. Arch Environ Contam Toxicol 17(2):213–218

    Article  CAS  Google Scholar 

  19. Guenter W, Hahn PH (1979) Fluorine toxicity and laying hen performance. Poult Sci 65:769–778

    Article  Google Scholar 

  20. Górecki H, Chojnacka K, Dobrzański Z, Kołacz R, Górecka H, Trziszka T (2006) The effect of phosphogypsum as the mineral feed additive on fluorine content in eggs and tissues of laying hens. Anim Feed Sci Technol 128(1):84–95

    Article  Google Scholar 

  21. Carriere D, Bird DM, Stamm JW (1987) Influence of a diet of fluoride-fed cockerels on reproductive performance of captive American kestrels. Environ Pollut 46:151–159

    Article  CAS  PubMed  Google Scholar 

  22. Whitford GM (1994) Intake and metabolism of fluoride. Adv Dent Res 8:5–14

    Article  CAS  PubMed  Google Scholar 

  23. Korr IM (1939) The osmotic function of the chicken kidney. J Cell Comp Physiol 13(2):175–193

    Article  CAS  Google Scholar 

  24. Shupe JL (1980) Clinicopathologic features of fluoride toxicosis in cattle. J Anim Sci 51:746–758

    Article  CAS  PubMed  Google Scholar 

  25. Kebreab E, France J, Kwakkel RP, Leeson S, Darmani H, Dijkstra J (2009) Development and evaluation of a dynamic model of calcium and phosphorus flows in layers. Poult Sci 88:680–689

    CAS  PubMed  Google Scholar 

  26. Hunton P (2005) Research on eggshell structure and quality: an historical overview. Revista Brasileira de Ciência Avícola 7(2):67–71

    Article  Google Scholar 

  27. Abdel-jabbar N, Al-asheh S (2009) Factorial design for the analysis of packed-bed sorption of copper using eggshell as a biosorbent. J Environ Protec Sci 3133–139

  28. Pasternak K, Truchlinski J (1999) Fluorine and its influence on animal bodies. Med Weter 55(10):643–645

    Google Scholar 

  29. Ensminger AH (1995) The concise encyclopedia “food and nutrition”. CRC, California

    Book  Google Scholar 

  30. Robin JP, Cherel Y, Girard H, Geloen A, Le Maho Y (1987) Uric acid and urea in relation to protein catabolism in long-term fasting geese. J Comp Physiol B 157:491–499

    Article  CAS  PubMed  Google Scholar 

  31. Arya HO, Wittwer F, Villa A, Ducom C (1990) Bovine fluorosis following volcanic activity in the southern Andes. Vet Rec 126:641–642

    Google Scholar 

  32. Tsunoda H, Makaya S, Sakurai S, Itai K, Yazaki K, Tatsumi M (1985) Studies on the effect of environmental fluoride on goats. In: Susheela AK (ed) Fluoride toxicity. International Society for Fluoride Research, New Delhi

    Google Scholar 

  33. Yuan C, Song HH, Jiang YJ, Azzam MMM, Zhu S, Zou XT (2013) Effects of lead contamination in feed on laying performance, lead retention of organs and eggs, protein metabolism, and hormone levels of laying hens. The Journal of Applied Poultry Research 22(4):878–884

    Article  CAS  Google Scholar 

  34. Ramaswamy M, Thangavel P, Panneer Selvam N (1999) Glutamic oxaloacetic transaminase (GOT) and glutamic pyruvic transaminase (GPT) enzyme activities in different tissues of Sarotherodon mossambicus (Peters) exposed to a carbamate pesticide, carbaryl. Pestic Sci 55:1217–1221

    Article  CAS  Google Scholar 

  35. Gaim K, Gebru G, Abba S (2015) The effect of arsenic on liver tissue of experimental animals (fishes and mice)—a review article. International Journal of Scientific and Research Publications 5(5):1–9

    Google Scholar 

Download references

Acknowledgements

This research was funded by the earmarked fund for Modern Argo-Industry Technology Research System of China (No. CARS-41-K17) and the National Key Technology Research and Development Program (No. 2014BAD13B04).

Author information

Authors and Affiliations

  1. Feed Science Institute, College of Animal Science, Zhejiang University, Hangzhou, 310058, China

    Liping Miao, Lanlan Li, Mingxing Qi, Minyao Zhou, Nannan Zhang & Xiaoting Zou

  2. Key Laboratory for Molecular Animal Nutrition of Ministry of Education, Feed Science Institute, College of Animal Science, Zhejiang University (Zijingang Campus), Hangzhou, 310058, China

    Xiaoting Zou

Authors
  1. Liping Miao
    View author publications

    Search author on:PubMed Google Scholar

  2. Lanlan Li
    View author publications

    Search author on:PubMed Google Scholar

  3. Mingxing Qi
    View author publications

    Search author on:PubMed Google Scholar

  4. Minyao Zhou
    View author publications

    Search author on:PubMed Google Scholar

  5. Nannan Zhang
    View author publications

    Search author on:PubMed Google Scholar

  6. Xiaoting Zou
    View author publications

    Search author on:PubMed Google Scholar

Corresponding author

Correspondence to Xiaoting Zou.

Ethics declarations

All animal procedures followed the guidelines of the National Institutes of Health Animal Care and Use Guidelines, Department of Environment, Forests, and Climate Change.

Conflict of Interest

The authors declare that they have no conflict of interest.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Miao, L., Li, L., Qi, M. et al. Effects of Excess Dietary Fluoride on Serum Biochemical Indices, Egg Quality, and Concentrations of Fluoride in Soft Organs, Eggs, and Serum of Laying Hens. Biol Trace Elem Res 180, 146–152 (2017). https://doi.org/10.1007/s12011-017-0973-7

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12011-017-0973-7

Keywords