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Analysis of the Interactions Between Thioredoxin and 20 Selenoproteins in Chicken

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Abstract

Thioredoxin (Trx) is a small molecular protein with complicated functions in a number of processes, including inflammation, apoptosis, embryogenesis, cardiovascular disease, and redox regulation. Some selenoproteins, such as glutathione peroxidase (Gpx), iodothyronine deiodinase (Dio), and thioredoxin reductase (TR), are involved in redox regulation. However, whether there are interactions between Trx and selenoproteins is still not known. In the present paper, we used a Modeller, Hex 8.0.0, and the KFC2 Server to predict the interactions between Trx and selenoproteins. We used the Modeller to predict the target protein in objective format and assess the accuracy of the results. Molecular interaction studies with Trx and selenoproteins were performed using the molecular docking tools in Hex 8.0.0. Next, we used the KFC2 Server to further test the protein binding sites. In addition to the selenoprotein physiological functions, we also explored potential relationships between Trx and selenoproteins beyond all the results we got. The results demonstrate that Trx has the potential to interact with 19 selenoproteins, including iodothyronine deiodinase 1 (Dio1), iodothyronine deiodinase 3 (Dio3), glutathione peroxidase 1 (Gpx1), glutathione peroxidase 2 (Gpx2), glutathione peroxidase 3 (Gpx3), glutathione peroxidase 4 (Gpx4), selenoprotein H (SelH), selenoprotein I (SelI), selenoprotein M (SelM), selenoprotein N (SelN), selenoprotein T (SelT), selenoprotein U (SelU), selenoprotein W (SelW), selenoprotein 15 (Sep15), methionine sulfoxide reductase B (Sepx1), selenophosphate synthetase 1 (SPS1), TR1, TR2, and TR3, among which TR1, TR2, TR3, SPS1, Sep15, SelN, SelM, SelI, Gpx2, Gpx3, Gpx4, and Dio3 exhibited intense correlations with Trx. However, additional experiments are needed to verify them.

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Abbreviations

Dio1:

Iodothyronine deiodinase 1

Dio2:

Iodothyronine deiodinase 2

Dio3:

Iodothyronine deiodinase 3

Gpx1:

Glutathione peroxidase 1

Gpx2:

Glutathione peroxidase 2

Gpx3:

Glutathione peroxidase 3

Gpx4:

Glutathione peroxidase 4

SelH:

Selenoprotein H

SelI:

Selenoprotein I

SelM:

Selenoprotein M

SelN:

Selenoprotein N

SelO:

Selenoprotein O

Sep15:

Selenoprotein 15

SelU:

Selenoprotein U

SelW:

Selenoprotein W

Sepx1/MrsB:

Methionine sulfoxide reductase B

SPS1:

Selenophosphate Synthetase 1

SPS2:

Selenophosphate Synthetase2

TR1:

Thioredoxin reductase 1

TR2:

Thioredoxin reductase 2

TR3:

Thioredoxin reductase 3

Trx:

Thioredoxin

Sec:

Selenocysteine

GSH:

Glutathione

ER:

Endoplasmic Reticulum

Rdx:

Radixin

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Acknowledgements

This study was supported by the National Natural Science Foundation of China (31402267), the Heilongjiang Province Science Foundation for Youths (QC2014C015), the Heilongjiang Postdoctoral Fund under Grant LBH-Z13028, the Foundation for Young Talents in Higher Education of Heilongjiang, China under Grant UNPYSCT-2015009, the “Young Talents” Project of Northeast Agricultural University under Grant 14QC20, and the Degree and graduate education teaching reform project in Heilongjiang province (JGXM_HLJ_201676).

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  1. College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, People’s Republic of China

    Qi Liu, Jie Yang, Jingzeng Cai, Yilin Luan, Hamid Sattar, Man Liu, Shiwen Xu & Ziwei Zhang

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  1. Qi Liu
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Correspondence to Ziwei Zhang.

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Liu, Q., Yang, J., Cai, J. et al. Analysis of the Interactions Between Thioredoxin and 20 Selenoproteins in Chicken. Biol Trace Elem Res 179, 304–317 (2017). https://doi.org/10.1007/s12011-017-0961-y

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