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Low Iodine in the Follicular Lumen Caused by Cytoplasm Mis-localization of Sodium Iodide Symporter may Induce Nodular Goiter

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Abstract

Iodine is a key ingredient in the synthesis of thyroid hormones and also a major factor in the regulation of thyroid function. A local reduction of iodine content in follicular lumen leads to overexpression of local thyroid-stimulating hormone receptor (TSHr), which in turn excessively stimulates the regional thyroid tissue, and result in the formation of nodular goiter. In this study, we investigated the relationship between iodine content and sodium iodide symporter (NIS) expression by using the clinical specimens from patients with nodular goiter and explored the pathogenesis triggered by iodine deficiency in nodular goiter. In total, 28 patients were clinically histopathologically confirmed to have nodular goiter and the corresponding adjacent normal thyroid specimens were harvested simultaneously. Western blot and immunohistochemistry were performed to assay NIS expression and localization in thyrocytes of both nodular goiter and adjacent normal thyroid tissues. NIS expression mediated by iodine in follicular lumen was confirmed by follicular model in vitro. Meanwhile, radioscan with iodine-131were conducted on both nodular goiter and adjacent normal thyroid. Our data showed that NIS expression in nodular goiter was significantly higher than that in adjacent normal tissues, which was associated with low iodine in the follicular lumen. Abnormal localization of NIS and lower amount of radioactive iodine-131 were also found in nodular goiter. Our data implied that low iodine in the follicular lumen caused by cytoplasm mis-localization of NIS may induce nodular goiter.

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Authors and Affiliations

  1. Department of Endocrinology, The Second Affiliated Hospital of Fujian Medical University, No. 34 North Zhongshan Road, Quanzhou, Fujian, 362000, People’s Republic of China

    Huibin Huang, Yaxiong Shi, Bo Liang, Huiyao Cai & Qingyan Cai

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  1. Huibin Huang
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  2. Yaxiong Shi
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  3. Bo Liang
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  4. Huiyao Cai
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  5. Qingyan Cai
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Correspondence to Huibin Huang.

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The authors declare that they have no conflict of interest.

Funding

This work was supported by the Natural Science Foundation of China (81370886), Natural Science Foundation of Fujian (2012J01332), Key Scientific Project of Fujian Province (2014Y0017), and Innovative Medical Research Project of Fujian Province (2012-CXB-24).

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Huang, H., Shi, Y., Liang, B. et al. Low Iodine in the Follicular Lumen Caused by Cytoplasm Mis-localization of Sodium Iodide Symporter may Induce Nodular Goiter. Biol Trace Elem Res 179, 165–171 (2017). https://doi.org/10.1007/s12011-017-0960-z

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  • DOI: https://doi.org/10.1007/s12011-017-0960-z

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