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Pre-Clinical Study for the Antidiabetic Potential of Selenium Nanoparticles


This research was delineated to explore the efficacy of selenium nanoparticles delivered in liposomes (L-Se) in the mitigation of type-2 diabetes mellitus. Adult female Wistar rats were assigned into four groups: group I, the normal control group in which the rats received normal saline solution orally; group II, the diabetic control group in which the rats were injected intraperitoneally with a single dose of streptozotocin (STZ) for induction of diabetes; group III, the metformin (Met)-treated group in which the diabetic rats were treated orally with Met; and group IV, the L-Se-treated group in which the diabetic rats were treated orally with L-Se. All treatments were delivered for 21 days. Blood and pancreas tissue samples were obtained for biochemical analysis, immunohistochemical examinations, and histopathological investigation. The L-Se-treated group showed significant drop in serum glucose and pancreatic malondialdehyde (MDA), nitric oxide (NO), tumor necrosis factor-α (TNF-α), and prostaglandin F2α (PGF2α) levels associated with significant rise in serum insulin and pancreatic glutathione, superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), and glutathione reductase (GR) values, in addition to significant improvement in the immunohistochemical indices (insulin and glucagon). Aforementioned results are appreciated by the histopathological findings of pancreatic tissue. In conclusion, our data have brought about compelling evidence favoring the antidiabetic potency of elemental selenium nanoparticles delivered in liposomes through preservation of pancreatic β cell integrity with consequent increment of insulin secretion and in turn glucose depletion, repression of oxidative stress, potentiation of the antioxidant defense system, and inhibition of pancreatic inflammation.

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Advanced glycosylation end product


AMP-activated protein kinase


One-way analysis of variance


Activating transcription factor-3






cAMP responsive element binding protein


Diabetes mellitus


L-α-dipalmitoyl phosphatidyl choline


5,5′ dithiobis (2-nitrobenzoic acid)


Enzyme-linked immunosorbent assay


Glutathione peroxidase


Glutathione reductase


Oxidized glutathione




Interleukin-1 beta






Inducible nitric oxide synthase


Nuclear factor of kappa light polypeptide gene enhancer in B cell inhibitor alpha


Insulin promoter factor 1


c-Jun N-terminal kinase 1/2




Selenium nanoparticles delivered in liposomes


Mitogen-activated protein kinase





Na2SeO3 :

Sodium selenite


Nitroblue tetrazolium


Nuclear factor kappa B


Nitric oxide


Peroxidase anti-peroxidase


Prostaglandin E2


Prostaglandin F2α


Reactive oxygen species




Superoxide dismutase


Statistical package for the social sciences program




Type 2 diabetes mellitus


Transmission electron microscope


Tumor necrosis factor-α


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Correspondence to Hanaa H. Ahmed.

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All animals received care in compliance with the Egyptian rules for animal experiments which were approved by the Ethical Committee of Medical Research of the National Research Centre, Giza, Egypt.

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Ahmed, H.H., Abd El-Maksoud, M.D., Abdel Moneim, A.E. et al. Pre-Clinical Study for the Antidiabetic Potential of Selenium Nanoparticles. Biol Trace Elem Res 177, 267–280 (2017).

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