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Pre-Clinical Study for the Antidiabetic Potential of Selenium Nanoparticles

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Abstract

This research was delineated to explore the efficacy of selenium nanoparticles delivered in liposomes (L-Se) in the mitigation of type-2 diabetes mellitus. Adult female Wistar rats were assigned into four groups: group I, the normal control group in which the rats received normal saline solution orally; group II, the diabetic control group in which the rats were injected intraperitoneally with a single dose of streptozotocin (STZ) for induction of diabetes; group III, the metformin (Met)-treated group in which the diabetic rats were treated orally with Met; and group IV, the L-Se-treated group in which the diabetic rats were treated orally with L-Se. All treatments were delivered for 21 days. Blood and pancreas tissue samples were obtained for biochemical analysis, immunohistochemical examinations, and histopathological investigation. The L-Se-treated group showed significant drop in serum glucose and pancreatic malondialdehyde (MDA), nitric oxide (NO), tumor necrosis factor-α (TNF-α), and prostaglandin F2α (PGF2α) levels associated with significant rise in serum insulin and pancreatic glutathione, superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), and glutathione reductase (GR) values, in addition to significant improvement in the immunohistochemical indices (insulin and glucagon). Aforementioned results are appreciated by the histopathological findings of pancreatic tissue. In conclusion, our data have brought about compelling evidence favoring the antidiabetic potency of elemental selenium nanoparticles delivered in liposomes through preservation of pancreatic β cell integrity with consequent increment of insulin secretion and in turn glucose depletion, repression of oxidative stress, potentiation of the antioxidant defense system, and inhibition of pancreatic inflammation.

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Abbreviations

AGE:

Advanced glycosylation end product

AMPK:

AMP-activated protein kinase

ANOVA:

One-way analysis of variance

ATF-3:

Activating transcription factor-3

CAT:

Catalase

COX-2:

Cyclooxygenase-2

CREB:

cAMP responsive element binding protein

DM:

Diabetes mellitus

DPPC:

L-α-dipalmitoyl phosphatidyl choline

DTNB:

5,5′ dithiobis (2-nitrobenzoic acid)

ELISA:

Enzyme-linked immunosorbent assay

GPx:

Glutathione peroxidase

GR:

Glutathione reductase

GSSG:

Oxidized glutathione

IFN-γ:

Interferon-gamma

IL-1β:

Interleukin-1 beta

IL-6:

Interleukin-6

IL-10:

Interleukin-10

iNOS:

Inducible nitric oxide synthase

IK-Bα:

Nuclear factor of kappa light polypeptide gene enhancer in B cell inhibitor alpha

Ipf1:

Insulin promoter factor 1

JNK1/2:

c-Jun N-terminal kinase 1/2

LPS:

Lipopolysaccharides

L-Se:

Selenium nanoparticles delivered in liposomes

MAPK:

Mitogen-activated protein kinase

MDA:

Malondialdehyde

Met:

Metformin

Na2SeO3 :

Sodium selenite

NBT:

Nitroblue tetrazolium

NF-κB:

Nuclear factor kappa B

NO:

Nitric oxide

PAP:

Peroxidase anti-peroxidase

PGE2:

Prostaglandin E2

PGF2α:

Prostaglandin F2α

ROS:

Reactive oxygen species

Se:

Selenium

SOD:

Superoxide dismutase

SPSS:

Statistical package for the social sciences program

STZ:

Streptozotocin

T2DM:

Type 2 diabetes mellitus

TEM:

Transmission electron microscope

TNF-α:

Tumor necrosis factor-α

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Authors and Affiliations

  1. Hormones Department, National Research Centre, 33 EL Bohouth st. (former EL Tahrir st.) Dokki, P.O. 12622, Giza, Egypt

    Hanaa H. Ahmed & Hadeer A. Aglan

  2. Biochemistry Department, National Research Centre, Giza, Egypt

    Mohamed Diaa Abd El-Maksoud

  3. Zoology and Entomology Department, Faculty of Science, Helwan University, Cairo, Egypt

    Ahmed E. Abdel Moneim

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  1. Hanaa H. Ahmed
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Correspondence to Hanaa H. Ahmed.

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All animals received care in compliance with the Egyptian rules for animal experiments which were approved by the Ethical Committee of Medical Research of the National Research Centre, Giza, Egypt.

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Ahmed, H.H., Abd El-Maksoud, M.D., Abdel Moneim, A.E. et al. Pre-Clinical Study for the Antidiabetic Potential of Selenium Nanoparticles. Biol Trace Elem Res 177, 267–280 (2017). https://doi.org/10.1007/s12011-016-0876-z

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