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Effect of Vanadium and Tea Polyphenols on Intestinal Morphology, Microflora and Short-Chain Fatty Acid Profile of Laying Hens

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Abstract

Vanadium (V) is a trace element which can induce dysfunction of gastro-intestine and egg quality deterioration of laying hens. This study was conducted to determine the effect of tea polyphenols (TP) on intestinal morphology, microflora, and short-chain fatty acid (SCFA) profile of laying hens fed vanadium containing diets. A total of 120 Lohman laying hens (67-week-old) were randomly divided into 4 groups with 6 replicates and 5 birds each for a 35-day feeding trial. The dietary treatments were as follows: (1) control (CON), fed a basal diet; (2) vanadium treatment (V10), CON +10 mg V/kg; (3) TP treatment 1 (TP1): V10 + 600 mg TP/kg; (4) TP treatment 2 (TP2): V10 + 1000 mg TP/kg. Fed 10 mg V/kg diets to laying hens did not affect the cecum flora diversity index (H), degree of homogeneity (EH), and richness (S), but hens fed TP2 diet decreased the H, EH, and S (P < 0.05). The cecum butyrate acid concentration was lower in V10 treatment and higher in TP2 treatment (P < 0.05). Addition of 10 mg/kg V resulted in an increased (P < 0.01) duodenal cell apoptosis rate, and 1000 mg/kg TP supplementation overcame (P < 0.01) this reduction effect induced by vanadium. The results indicated that supplementation of 10 mg/kg vanadium increased duodenal cell apoptosis and reduced cecum butyrate acid content. Addition of 1000 mg/kg TP increased the SCFA production to affect cecum flora ecology and protected the duodenal cell from excess apoptosis caused by vanadium.

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Acknowledgments

This project was fanatically supported by National Natural Science Foundation of China (31402031) and Sichuan Provincial Science and Technology Projects (13ZB0290, 2014BAD13B04, 2014NZ0043, 2014NZ0002, 2013NZ0054).

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Authors and Affiliations

  1. Institute of Animal Nutrition, Sichuan Agricultural University, Kechuang Feed Industry in Sichuan, Chengdu, Sichuan, 611130, China

    Z. H. Yuan, J. P. Wang, K. Y. Zhang, X. M. Ding, S. P. Bai, Q. F. Zeng, Y. Xuan & Z. W. Su

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  1. Z. H. Yuan
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Correspondence to J. P. Wang.

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Yuan, Z.H., Wang, J.P., Zhang, K.Y. et al. Effect of Vanadium and Tea Polyphenols on Intestinal Morphology, Microflora and Short-Chain Fatty Acid Profile of Laying Hens. Biol Trace Elem Res 174, 419–427 (2016). https://doi.org/10.1007/s12011-016-0721-4

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