Skip to main content
SpringerLink
Log in

Selenium Deficiency Influences the Expression of Selenoproteins and Inflammatory Cytokines in Chicken Aorta Vessels

  • Published:
Biological Trace Element Research Aims and scope Submit manuscript

Abstract

Selenium deficiency is known to cause cardiovascular diseases. However, the role of Se deficiency in causing oxidative damage and inflammation injury to the aorta vessels of chickens is not well known. In the present study, 180 1-day-old chickens were randomly divided into two groups, a low-Se group (L group) and a control-Se group (C group). The messenger RNA (mRNA) levels of 25 selenoproteins, the mRNA and protein expression levels of inflammatory cytokines (including NF-κB, TNF-α, COX-2, and PTGES), and the antioxidant levels in chicken aorta vessels were examined. The results showed that the mRNA levels of 25 selenoproteins and the activity of Gpx were decreased, while the mRNA and protein expression levels of inflammatory cytokines and the MDA content were increased by Se deficiency in chicken aorta vessels. The data from the present study indicated that Se deficiency decreases the expression of selenoproteins, reduces antioxidant function, and increases the expression of inflammatory factors in chicken aorta vessels.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  1. Tanguy S, Rakotovao A, Jouan MG, et al. (2011) Dietary selenium intake influences Cx43 dephosphorylation, TNF-alpha expression and cardiac remodeling after reperfused infarction. Mol Nutr Food Res 55:522–529

    Article  CAS  PubMed  Google Scholar 

  2. Flores-Mateo G, Navas-Acien A, Pastor-Barriuso R, et al. (2006) Selenium and coronary heart disease: a meta-analysis. Am J Clin Nutr 84:762–773

    CAS  PubMed  PubMed Central  Google Scholar 

  3. Reeves MA, Hoffmann PR (2009) The human selenoproteome: recent insights into functions and regulation. Cell Mol Life Sci 66:2457–2478

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  4. Maseko T, Howell K, Dunshea FR, et al. (2014) Selenium-enriched Agaricus bisporus increases expression and activity of glutathione peroxidase-1 and expression of glutathione peroxidase-2 in rat colon. Food Chem 146:327–333

    Article  CAS  PubMed  Google Scholar 

  5. Liu CP, Fu J, Lin SL, et al. (2014) Effects of dietary selenium deficiency on mRNA levels of twenty-one selenoprotein genes in the liver of layer chicken. Biol Trace Elem Res 159:192–198

    Article  CAS  PubMed  Google Scholar 

  6. Yao HD, Wu Q, Zhang ZW, et al. (2013) Gene expression of endoplasmic reticulum resident selenoproteins correlates with apoptosis in various muscles of Se-deficient chicks. J Nutr 143:613–619

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  7. Yao HD, Wu Q, Zhang ZW, et al. (2013) Selenoprotein W serves as an antioxidant in chicken myoblasts. Biochim Biophys Acta 1830:3112–3120

    Article  CAS  PubMed  Google Scholar 

  8. Tang R, Liu H, Wang T, et al. (2005) Mechanisms of selenium inhibition of cell apoptosis induced by oxysterols in rat vascular smooth muscle cells. Arch Biochem Biophys 441:16–24

    Article  CAS  PubMed  Google Scholar 

  9. Forgione MA, Cap A, Liao R, et al. (2002) Heterozygous cellular glutathione peroxidase deficiency in the mouse: abnormalities in vascular and cardiac function and structure. Circulation 106:1154–1158

    Article  CAS  PubMed  Google Scholar 

  10. Libby P, Ridker PM, Maseri A (2002) Inflammation and atherosclerosis. Circulation 105:1135–1143

    Article  CAS  PubMed  Google Scholar 

  11. Hoffmann PR, Jourdan-Le SC, Hoffmann FW, et al. (2007) A role for dietary selenium and selenoproteins in allergic airway inflammation. J Immunol 179:3258–3267

    Article  CAS  PubMed  Google Scholar 

  12. Hall JA, Bobe G, Vorachek WR, et al. (2014) Effect of supranutritional organic selenium supplementation on postpartum blood micronutrients, antioxidants, metabolites, and inflammation biomarkers in selenium-replete dairy cows. Biol Trace Elem Res 161:272–287

    Article  CAS  PubMed  Google Scholar 

  13. Zhao J, Xing H, Liu C et al (2015) Effect of selenium deficiency on nitric oxide and heat shock proteins in chicken erythrocytes. Biol Trace Elem Res

  14. Wu Q, Yao HD, Tan SR, et al. (2014) Possible correlation of selenoprotein W with inflammation factors in chicken skeletal muscles. Biol Trace Elem Res 161:167–172

    Article  CAS  PubMed  Google Scholar 

  15. Vunta H, Belda BJ, Arner RJ, et al. (2008) Selenium attenuates pro-inflammatory gene expression in macrophages. Mol Nutr Food Res 52:1316–1323

    Article  CAS  PubMed  Google Scholar 

  16. Flohe L, Gunzler WA (1984) Assays of glutathione peroxidase. Methods Enzymol 105:114–121

    Article  CAS  PubMed  Google Scholar 

  17. Esterbauer H, Cheeseman KH (1990) Determination of aldehydic lipid peroxidation products: malonaldehyde and 4-hydroxynonenal. Methods Enzymol 186:407–421

    Article  CAS  PubMed  Google Scholar 

  18. Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254

    Article  CAS  PubMed  Google Scholar 

  19. Peirson SN, Butler JN, Foster RG (2003) Experimental validation of novel and conventional approaches to quantitative real-time PCR data analysis. Nucleic Acids Res 31:e73

    Article  PubMed  PubMed Central  Google Scholar 

  20. Pfaffl MW (2001) A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res 29:e45

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  21. Zhang JL, Li JL, Huang XD, et al. (2012) Dietary selenium regulation of transcript abundance of selenoprotein N and selenoprotein W in chicken muscle tissues. Biometals 25:297–307

    Article  PubMed  Google Scholar 

  22. Shi L, Yue W, Zhang C, et al. (2010) Effects of maternal and dietary selenium (Se-enriched yeast) on oxidative status in testis and apoptosis of germ cells during spermatogenesis of their offspring in goats. Anim Reprod Sci 119:212–218

    Article  CAS  PubMed  Google Scholar 

  23. Liu Y, Zhao H, Zhang Q, et al. (2012) Prolonged dietary selenium deficiency or excess does not globally affect selenoprotein gene expression and/or protein production in various tissues of pigs. J Nutr 142:1410–1416

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  24. Witte KK, Clark AL, Cleland JG (2001) Chronic heart failure and micronutrients. J Am Coll Cardiol 37:1765–1774

    Article  CAS  PubMed  Google Scholar 

  25. Thomas JP, Geiger PG, Girotti AW (1993) Lethal damage to endothelial cells by oxidized low density lipoprotein: role of selenoperoxidases in cytoprotection against lipid hydroperoxide- and iron-mediated reactions. J Lipid Res 34:479–490

    CAS  PubMed  Google Scholar 

  26. Huang K, Liu H, Chen Z, et al. (2002) Role of selenium in cytoprotection against cholesterol oxide-induced vascular damage in rats. Atherosclerosis 162:137–144

    Article  CAS  PubMed  Google Scholar 

  27. Curran JE, Jowett JB, Elliott KS, et al. (2005) Genetic variation in selenoprotein S influences inflammatory response. Nat Genet 37:1234–1241

    Article  CAS  PubMed  Google Scholar 

  28. Loflin J, Lopez N, Whanger PD, et al. (2006) Selenoprotein W during development and oxidative stress. J Inorg Biochem 100:1679–1684

    Article  CAS  PubMed  Google Scholar 

  29. Jianhua H, Ohtsuka A, Hayashi K (2000) Selenium influences growth via thyroid hormone status in broiler chickens. Br J Nutr 84:727–732

    CAS  PubMed  Google Scholar 

  30. Liang Y, Lin SL, Wang CW, et al. (2014) Effect of selenium on selenoprotein expression in the adipose tissue of chickens. Biol Trace Elem Res 160:41–48

    Article  CAS  PubMed  Google Scholar 

  31. Lin SL, Wang CW, Tan SR, et al. (2014) Selenium deficiency inhibits the conversion of thyroidal thyroxine (T4) to triiodothyronine (T3) in chicken thyroids. Biol Trace Elem Res 161:263–271

    Article  CAS  PubMed  Google Scholar 

  32. Cogswell JP, Godlevski MM, Wisely GB, et al. (1994) NF-kappa B regulates IL-1 beta transcription through a consensus NF-kappa B binding site and a nonconsensus CRE-like site. J Immunol 153:712–723

    CAS  PubMed  Google Scholar 

  33. Shalini S, Bansal MP (2007) Alterations in selenium status influences reproductive potential of male mice by modulation of transcription factor NFkappaB. Biometals 20:49–59

    Article  CAS  PubMed  Google Scholar 

  34. Skoog T, Dichtl W, Boquist S, et al. (2002) Plasma tumour necrosis factor-alpha and early carotid atherosclerosis in healthy middle-aged men. Eur Heart J 23:376–383

    Article  CAS  PubMed  Google Scholar 

  35. Ozbal S, Erbil G, Kocdor H, et al. (2008) The effects of selenium against cerebral ischemia-reperfusion injury in rats. Neurosci Lett 438:265–269

    Article  PubMed  Google Scholar 

  36. Surh YJ, Chun KS, Cha HH, et al. (2001) Molecular mechanisms underlying chemopreventive activities of anti-inflammatory phytochemicals: down-regulation of COX-2 and iNOS through suppression of NF-kappa B activation. Mutat Res 480-481:243–268

    Article  CAS  PubMed  Google Scholar 

  37. Dakin SG, Dudhia J, Werling NJ, et al. (2012) Inflamm-aging and arachidonic acid metabolite differences with stage of tendon disease. PLoS one 7:e48978

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  38. Liu T, Laidlaw TM, Feng C, et al. (2012) Prostaglandin E2 deficiency uncovers a dominant role for thromboxane A2 in house dust mite-induced allergic pulmonary inflammation. Proc Natl Acad Sci U S A 109:12692–12697

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  39. Rimarachin JA, Jacobson JA, Szabo P, et al. (1994) Regulation of cyclooxygenase-2 expression in aortic smooth muscle cells. Arterioscler Thromb 14:1021–1031

    Article  CAS  PubMed  Google Scholar 

  40. Bishop-Bailey D, Pepper JR, Larkin SW, et al. (1998) Differential induction of cyclooxygenase-2 in human arterial and venous smooth muscle: role of endogenous prostanoids. Arterioscler Thromb Vasc Biol 18:1655–1661

    Article  CAS  PubMed  Google Scholar 

  41. Sheng PF, Jiang Y, Zhang ZW, et al. (2014) The effect of Se-deficient diet on gene expression of inflammatory cytokines in chicken brain. Biometals 27:33–43

    Article  CAS  PubMed  Google Scholar 

  42. Crack PJ, Taylor JM, Ali U, et al. (2006) Potential contribution of NF-kappaB in neuronal cell death in the glutathione peroxidase-1 knockout mouse in response to ischemia-reperfusion injury. Stroke 37:1533–1538

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

We thank Elsevier Language Editing Services in helping us correct the English language errors. This study was supported by the International (Regional) Cooperation and Exchange Projects of the National Natural Science Foundation of China (31320103920) and the National Natural Science Foundation of China (31272626).

Author information

Authors and Affiliations

  1. College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, People’s Republic of China

    Qiang Du, Haidong Yao, Linlin Yao, Ziwei Zhang & Shiwen Xu

  2. College of Food Science and Nutrition Engineering, China Agricultural University, Beijing, People’s Republic of China

    Xingen Lei

  3. Department of Animal Science, Cornell University, Ithaca, NY, USA

    Xingen Lei

Authors
  1. Qiang Du
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Haidong Yao
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Linlin Yao
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Ziwei Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Xingen Lei
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Shiwen Xu
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Xingen Lei or Shiwen Xu.

Additional information

All authors have read the manuscript and agreed to submit it in its current form for consideration for publication in the journal.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Du, Q., Yao, H., Yao, L. et al. Selenium Deficiency Influences the Expression of Selenoproteins and Inflammatory Cytokines in Chicken Aorta Vessels. Biol Trace Elem Res 173, 501–513 (2016). https://doi.org/10.1007/s12011-016-0676-5

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12011-016-0676-5

Keywords

Search

Navigation