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Storage of Heparinised Canine Whole Blood for the Measurement of Glutathione Peroxidase Activity

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Abstract

Glutathione peroxidase activity is used as a biomarker of selenium status in dogs. Freshly collected blood samples are usually measured, due to the lack of knowledge on the effect of storing the samples. This study investigated if the analysis of glutathione peroxidase activity in whole blood collected from dogs was affected by storage of between 5 and 164 days. Results indicated that glutathione peroxidase activity was more variable in the freshly analysed samples compared to the stored samples. Although the mean differences between fresh and stored samples were not always equal to zero, this is thought to be caused by the variability of reagent preparation rather than by storage, as no consistent increase or decrease in glutathione peroxidase activity was found. Therefore, it can be concluded that heparinised dog blood samples can be successfully stored up to 164 days before analysis of glutathione peroxidase activity.

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Acknowledgments

This study is part of a PhD project funded by the WALTHAM Centre for Pet Nutrition. The funder, other than the named author, had no role in the study design, data collection and analysis, interpretation of data, decision to publish or preparation of the manuscript.

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Authors and Affiliations

  1. Laboratory of Animal Nutrition, Faculty of Veterinary Medicine, Ghent University, Heidestraat 19, Merelbeke, Belgium

    Mariëlle van Zelst, Myriam Hesta & Geert P. J. Janssens

  2. WALTHAM Centre for Pet Nutrition, Freeby Lane, Waltham-on-the-Wolds, Leicestershire, UK

    Kerry Gray

Authors
  1. Mariëlle van Zelst
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  2. Myriam Hesta
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  3. Kerry Gray
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  4. Geert P. J. Janssens
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Corresponding author

Correspondence to Geert P. J. Janssens.

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Conflict of Interest

This study is part of a PhD project by MvZ funded by the WALTHAM Centre for Pet Nutrition (grant number not applicable). KG was employed by the WALTHAM Centre for Pet Nutrition at the time of the study. The other authors declare that they have no conflict of interest.

Ethical Approval

All applicable international, national, and institutional guidelines for the care and use of animals were followed. All procedures performed in this study were in accordance with the ethical standards of the institution (WALTHAM Center for Pet Nutrition, under Home Office Project License) at which the study was conducted.

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van Zelst, M., Hesta, M., Gray, K. et al. Storage of Heparinised Canine Whole Blood for the Measurement of Glutathione Peroxidase Activity. Biol Trace Elem Res 172, 361–363 (2016). https://doi.org/10.1007/s12011-015-0603-1

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  • DOI: https://doi.org/10.1007/s12011-015-0603-1

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