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Excessive Selenium Supplementation Induced Oxidative Stress and Endoplasmic Reticulum Stress in Chicken Spleen

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Abstract

Excessive selenium (Se) intake is harmful for animals and humans. The aim of the present study was to examine the effect of long-term excessive Se supplementation on oxidative stress and endoplasmic reticulum (ER) stress-related injuries in chicken spleen. A total of 180 1-day-old chickens were randomly divided into four groups with different Se dietary contents (0.2 mg/kg Se, 5 mg/kg Se, 10 mg/kg Se, or 15 mg/kg Se) for 45 days. Then, the levels of antioxidative enzymes, GPx, SOD, and MDA as well as the expression levels of GRP78, ARF6, caspase 3, caspase 12, and Bcl 2 in the spleen were determined at days 15, 30, and 45, respectively. The results showed that excessive Se treatment decreased the activities of GPx and SOD (P < 0.05) but increased the levels of MDA (P < 0.05) in a dose- and time-dependent manner. In addition, the ER stress genes GRP78 and ATF6 were highly expressed (P < 0.05), and the apoptosis genes caspase 3 and caspase 12 were increased, but Bcl 2 was decreased by Se treatment (P < 0.05). Correlation analysis showed that there was a high correlation between these biomarkers, which indicated that ER stress and ER stress-related apoptosis were correlated with oxidative stress. These results showed the important role of oxidative stress and ER stress in Se-related immune injuries in chicken.

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Acknowledgments

The present work was financially supported by Southwest University of Science and Technology (15zx7121) and Mianyang Science and Technology Project (14 N043). The authors thank the Elsevier English Language Editing System to correct grammatical, spelling, and other common errors.

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    Authors and Affiliations

    1. School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang, Sichuan, 621010, China

      Yachao Wang, Li Jiang, Yuanfeng Li & Jian He

    2. Engineering Research Center of Biomass Materials, Ministry of Education, Southwest University of Science and Technology, Mianyang, Sichuan, 621010, China

      Yachao Wang & Xuegang Luo

    3. School of Material Science and Engineering, Southwest University of Science and Technology, Mianyang, Sichuan, 621010, China

      Xuegang Luo

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    1. Yachao Wang
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    Correspondence to Xuegang Luo or Jian He.

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    Yachao Wang and Li Jiang should be considered as co-first authors

    Yachao Wang and Li Jiang contributed equally to this work.

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    Wang, Y., Jiang, L., Li, Y. et al. Excessive Selenium Supplementation Induced Oxidative Stress and Endoplasmic Reticulum Stress in Chicken Spleen. Biol Trace Elem Res 172, 481–487 (2016). https://doi.org/10.1007/s12011-015-0596-9

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    • DOI: https://doi.org/10.1007/s12011-015-0596-9

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