Serum Zinc Concentration and C-Reactive Protein in Individuals with Human Immunodeficiency Virus Infection: the Positive Living with HIV (POLH) Study
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Low zinc levels and chronic inflammation are common in individuals infected with human immunodeficiency virus (HIV). Zinc deficiency may promote systemic inflammation, but research on the role of zinc in inflammation among HIV-positive individuals taking account of anti-retroviral therapy is lacking. We assessed the association between serum zinc and C-reactive protein (CRP) concentration in a cohort of HIV-positive individuals. A cross-sectional survey was conducted among 311 HIV-positive individuals (177 men and 134 women) aged 18–60 years residing in Kathmandu, Nepal. High-sensitive or regular serum CRP concentrations were measured by the latex agglutination nephelometry or turbidimetric method, and zinc concentrations were measured by the atomic absorption method. Relationships were assessed using multiple linear regression analysis. The geometric means of zinc in men and women were 73.83 and 71.93 ug/dL, respectively, and of CRP were 1.64 and 0.96 mg/L, respectively. Mean serum CRP concentration was significantly decreased with increasing serum zinc concentration across zinc tertiles (P for trend = 0.010), with mean serum CRP concentration in the highest tertile of serum zinc concentration was 44.2 % lower than that in the lowest tertile. The mean serum CRP concentrations in men and women in the highest tertile of serum zinc concentrations were 30 and 35.9 % lower, respectively, than that in the lowest tertile (P for trend = 0.263 and 0.162, respectively). We found a significant inverse relation between log zinc and log CRP concentrations (beta for 1 unit change in log zinc; β = −1.79, p = 0.0003). Serum zinc concentration may be inversely associated with serum CRP concentration in HIV-positive individuals.
KeywordsZinc Inflammation HIV infection C-reactive protein Nepal
The authors would like to thank all of the participants for their valuable information, cooperation, and participation. The authors would also like to gratefully acknowledge the support and co-ordination of five local NGOs working with HIV-positive populations in the Kathmandu Valley, Nepal—Youth Vision, Sneha Samaj, Srijansil Mahila Samuha, SPARSHA Nepal, and Shakti Milan Samaj for recruiting participants and collecting information. This study was partially supported by the Grant-in-Aid for Young Scientists (B) (22790581), Japan Society for the Promotion of Science, The Ministry of Education, Culture, Sports, Science and Technology, Japan; Waseda University Grants for Special Research Projects, General Grant/Ippan Josei, Japan (2012A-101); and by the Grant for Research on Global Health and Medicine (No. 21A-2) from the National Center for Global Health and Medicine, Japan. The funding agency had no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; and in the decision to submit the paper for publication.
Compliance with Ethical Standards
Ethics Approval and Consent to Participate
The Ethics Committees of the Nepal Health Research Council, Kathmandu, Nepal; National Center for Global Health and Medicine, Japan; and Waseda University, Tokyo, Japan, approved the study protocol. The Institutional Review Board of the University of Massachusetts, Amherst, USA, also approved the study procedures. The participants provided their written informed consent.
Conflict of Interest
The authors declare that they have no competing interests.
- 5.Wittkop L, Bitard J, Lazaro E, Neau D, Bonnet F et al (2013) Effect of cytomegalovirus-induced immune response, self antigen-induced immune response, and microbial translocation on chronic immune activation in successfully treated HIV type 1-infected patients: the ANRS CO3 Aquitaine Cohort. J Infect Dis 207:622–627CrossRefPubMedGoogle Scholar
- 18.Friis H, Sandstrom B (2002) Zinc and HIV infection. In: Friis H (ed) Micronutrients and HIV infection: CRC series in modern nutrition. CRC Press, Boca Raton, Florida, pp 159–181Google Scholar
- 22.Edeas MA, Peltier E, Claise C, Khalfoun Y, Lindenbaum A (1996) Immunocytochemical study of uptake of exogenous carrier-free copper-zinc superoxide dismutase by peripheral blood lymphocytes. Cell Mol Biol (Noisy-le-grand) 42:1137–1143Google Scholar
- 25.Irlam JH, Visser MM, Rollins NN, Siegfried N (2010) Micronutrient supplementation in children and adults with HIV infection. Cochrane Database Syst Rev 12:CD003650Google Scholar
- 53.NCASC (2012) Nepal Country Progress Report 2012: to contribute to Global AIDS Response Progress Report 2012. National Centre for AIDS and STD Control, KathmanduGoogle Scholar
- 54.NCASC (2007) National estimates of HIV infections, Nepal. National Centre for AIDS and STD Control, KathmanduGoogle Scholar
- 58.Poudel-Tandukar K, Bertone-Johnson ER, Palmer PH, Poudel KC (2014) C-reactive protein and depression in persons with human immunodeficiency virus infection: the Positive Living with HIV (POLH) Study. Brain, Behavior, and Immunity 42:89–95Google Scholar
- 63.Poudel KC, Poudel-Tandukar K, Nakahara S, Yasuoka J, Jimba M (2011) Knowing the consequences of unprotected sex with seroconcordant partner is associated with increased safer sex intentions among HIV-positive men in Kathmandu, Nepal. J Health Popul Nutr 29:191–199CrossRefPubMedPubMedCentralGoogle Scholar
- 64.Wfood2 (1996) World Food 2 Computer Software Package. Version 1.0. The Regents of the University of California, Berkeley, CAGoogle Scholar