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The Role of Calcium in Ameliorating the Oxidative Stress of Fluoride in Rats

An Erratum to this article was published on 02 September 2015


The present study was carried out to investigate the effects of fluoride toxicity on some biochemical, hormonal, and histological parameters of female rats and the protective role of calcium against such effects. Adult female albino rats were divided into five groups; control group received distilled water for 60 days, calcium group received calcium carbonate with dose of 50 mg/kg three times per week for 60 days, fluoride group received sodium fluoride with dose of 20 mg/kg three times per week for 60 days, calcium + fluoride group received calcium carbonate (50 mg/kg) then after 2 h received sodium fluoride (20 mg/kg) three times per week for 60 days, and fluoride + calcium group received sodium fluoride (20 mg/kg) three times per week for 30 days then received calcium carbonate (50 mg/kg) three times per week for another 30 days. The results showed that the levels of thiobarbituric acid reactive substances, urea, creatinine, alkaline phosphatase, triiodothyronine, thyroxine, parathormone, phosphorous, magnesium, alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, and gamma glutamyl transferase were significantly increased in rats treated with fluoride while serum estradiol, calcium, and organ glutathione were significantly decreased. The histological examination of the femur bone revealed that fluoride treatment induced thinning of bone trabeculae with wilding of marrow space, demineralization, and loss of trabeculae interconnections. Also, the histological examination of hepatic and renal tissues of fluoride-treated rats showed some damages in these tissues while administration of calcium carbonate for 30 or 60 days during fluoride treatment minimized such damages. It could be concluded that administration of calcium to female rats can ameliorate the hazardous effects of fluoride observed in the biochemical, hormonal, and histological parameters.

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The author expresses her thanks to Dr. T.N. El-Balkiny, Pathology Department, National Cancer Institute, Cairo University, Egypt, for his help in the histological examinations of bone. Also, the author expresses her thanks to Prof. Dr. S.S. Abdelgayed, Professor of Veterinary Pathology, Faculty of Veterinary Medicine, Cairo University, Egypt, for her help in performing the histological examinations and interpretations of liver and kidney sections. The author expresses her thanks to Prof. Dr. N. I. Hazzaa, Professor of Radiobiology, Middle Eastern Regional Radioisotope Centre for the Arab Countries, Dokki, Giza, Egypt, for her help in revising this manuscript.

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Mohamed, N.E. The Role of Calcium in Ameliorating the Oxidative Stress of Fluoride in Rats. Biol Trace Elem Res 170, 128–144 (2016).

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  • Fluoride toxicity
  • Calcium
  • Oxidative stress
  • Thyroid hormones
  • Parathormone hormone
  • Kidney function
  • Liver function
  • Femur bone