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Biological Trace Element Research

, Volume 168, Issue 2, pp 508–515 | Cite as

Spectroscopic Evaluation of DNA–Borate Interactions

  • Ayse Ozdemir
  • Omer Faruk Sarioglu
  • Turgay TekinayEmail author
Article
  • 166 Downloads

Abstract

We describe the binding characteristics of two natural borates (colemanite and ulexite) to calf thymus DNA by UV–vis absorbance spectroscopy, circular dichroism (CD) spectroscopy, Fourier transform infrared (FT-IR) spectroscopy, and a competitive DNA binding assay. Our results suggest that colemanite and ulexite interact with calf thymus DNA under a non-intercalative mode of binding and do not alter the secondary structure of the DNA helix. The FT-IR spectroscopy results indicate that the two borates might interact with DNA through sugar-phosphate backbone binding.

Keywords

Calf thymus DNA DNA binding Spectroscopy Boron Deoxyribose sugar 

Abbreviations

CD

Circular dichroism

Col

Colemanite

CT-DNA

Calf thymus DNA

FT-IR

Fourier transform infrared

Ule

Ulexite

UV–vis

Ultraviolet–visible

Notes

Acknowledgments

The authors are thankful to the Turkish National Boron Research Institute (Ulusal Bor Araştırma Enstitüsü (BOREN), Ankara, Turkey) for financial support through Research Grant No. 2012.ç0356 and to E. Kalyoncu, A. D. Özkan, and R. T. Gursacli for their help and support in conducting the experiments described. A. Ozdemir is supported by TUBITAK BIDEB (2211) PhD fellowship. O.F. Sarioglu acknowledges TUBITAK BIDEB (2211-C) for National PhD Scholarship.

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Ayse Ozdemir
    • 1
    • 2
  • Omer Faruk Sarioglu
    • 1
    • 2
  • Turgay Tekinay
    • 3
    • 4
    Email author
  1. 1.Institute of Materials Science & NanotechnologyBilkent UniversityAnkaraTurkey
  2. 2.UNAM-National Nanotechnology Research CenterBilkent UniversityAnkaraTurkey
  3. 3.Department of Medical Biology and Genetics, Faculty of MedicineGazi UniversityAnkaraTurkey
  4. 4.Life Sciences Application and Research CenterGazi UniversityAnkaraTurkey

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