Low Molybdenum State Induced by Tungsten as a Model of Molybdenum Deficiency in Rats
- 216 Downloads
Organ molybdenum (Mo) concentration and the activity of hepatic sulfite oxidase and xanthine oxidase were compared in tungsten-administered rats as well as rats fed with a low Mo diet to evaluate the use of tungsten-administered rats as a model of Mo deficiency. Twenty-four male 6-week-old Wistar rats were divided into four groups according to diet (AIN93G diet (control diet) or the control diet minus ammonium molybdate (low Mo diet)) and drinking water (deionized water or deionized water containing 200 μg/mL tungsten in the form of sodium tungstate). Mo content in the control and low Mo diets were 196 and 42 ng/g, respectively. Intake of the low Mo diet significantly reduced the Mo content of several organs and serum. Decrease in hepatic sulfite oxidase activity was also induced by the low Mo diet. The administration of tungsten induced marked decreases in organ Mo content and the activity of hepatic sulfite oxidase and xanthine oxidase. These decreases induced by tungsten administration were more pronounced than those induced by just a low Mo diet. Serum uric acid was also reduced by tungsten administration irrespective of Mo intake. Although a comparatively high accumulation of tungsten (3 to 9 μg/g) was observed in the kidneys and liver, adverse effects of tungsten accumulation on liver and kidney function were not observed in serum biochemical tests. These results indicate that tungsten-administered animals may be used as a model of Mo deficiency.
KeywordsMolybdenum deficiency Tungsten Sulfite oxidase Xanthine oxidase Serum uric acid
- 3.Yoshihara K, Fukunaga K, Yoshida M (2007) Effect of dietary molybdenum level on tissue and serum molybdenum concentrations in rats. Trace Nutrients Res 24:120–123Google Scholar
- 5.Herken EN, Kocamaz E, Erel O, Celik H, Kucukatay V (2009) Effect of sulfite treatment on total antioxidant capacity, total oxidant status, lipid hydroperoxide, and total free sulfydryl groups contents in normal and sulfite oxidase-deficient rat plasma. Cell Biol Toxicol 25:355–362. doi: 10.1007/s10565-008-9089-3 CrossRefPubMedGoogle Scholar
- 17.Tyrrell J, Galloway TS, Abo-Zaid G, Melzer D, Depledge MH, Osborne NJ (2013) High urinary tungsten concentration is associated with stroke in the National Health and Nutrition Examination Survey 1999–2010. PLoS One 8:e77546. doi: 10.1371/journal.pone.0077546 CrossRefPubMedCentralPubMedGoogle Scholar