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Effect of Physalis peruviana L. on Cadmium-Induced Testicular Toxicity in Rats

Biological Trace Element Research volume 159, pages 278–287 (2014)Cite this article

Abstract

Cadmium (Cd) stimulates the production of reactive oxygen species and causes tissue damage. We investigated here the protective effect of Physalis peruviana L. (family Solanaceae) against cadmium-induced testes toxicity in rats. Twenty-eight Wistar albino rats were used. They were divided into four groups (n = 7). Group 1 was used as control. Group 2 was intraperitoneally injected with 6.5 mg/kg body weight (bwt) of cadmium chloride for 5 days. Group 3 was orally treated with 200 mg/kg bwt of methanolic extract of physalis (MEPh). Group 4 was pretreated with MEPh before cadmium for 5 days. Changes in body and testes weights were determined. Oxidative stress markers, antioxidant enzymes, and testosterone level were measured. Histopathological changes of testes were examined, and the immunohistochemical staining for the proapoptotic (caspase-3) protein was performed. The injection of cadmium caused a significant decrease in body weight, while a significant increase in testes weight and testes weight index was observed. Pretreatment with MEPh was associated with significant reduction in the toxic effects of Cd as shown by reduced testicular levels of malondialdehyde, nitric oxide, and caspase-3 expression and increased glutathione content, and the activities of superoxide dismutase, catalase, glutathione reductase, glutathione peroxidase, and testosterone were also increased. Testicular histopathology showed that Cd produced an extensive germ cell apoptosis, and the pretreatment of MEPh in Cd-treated rats significantly reduced Cd-induced testicular damage. On the basis of the above results, it can be hypothesized that P. peruviana L. has a protective effect against cadmium-induced testicular oxidative stress and apoptosis in the rat.

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Abbreviations

Cas-3:

Caspase-3

CAT:

Catalase

Cd:

Cadmium

CdCl2 :

Cadmium chloride

GPx:

Glutathione peroxidase

GR:

Glutathione reductase

GSH:

Glutathione

MDA:

Malondialdehyde

MEPh:

Methanolic extract of physalis

NO:

Nitric oxide

ROS:

Reactive oxygen species

SOD:

Superoxide dismutase

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Authors and Affiliations

  1. Faculty of Biotechnology, October University for Modern Science and Arts (MSA), Giza, Egypt

    Mohamed S. Othman, Ahmed Nada & Hassan S. Zaki

  2. Department of Zoology and Entomology, Faculty of Science, Helwan University, Cairo, Egypt

    Ahmed E. Abdel Moneim

  3. Department of Biochemistry and Molecular Biology, Asturias Institute of Biotechnology, University of Oviedo, Oviedo, Spain

    Ahmed E. Abdel Moneim

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  1. Mohamed S. Othman
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  2. Ahmed Nada
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  3. Hassan S. Zaki
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  4. Ahmed E. Abdel Moneim
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Correspondence to Ahmed E. Abdel Moneim.

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Othman, M.S., Nada, A., Zaki, H.S. et al. Effect of Physalis peruviana L. on Cadmium-Induced Testicular Toxicity in Rats. Biol Trace Elem Res 159, 278–287 (2014). https://doi.org/10.1007/s12011-014-9955-1

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