NE Strengthens the Immunosuppression Induced by AlCl3 Through β2-AR/cAMP Pathway in Cultured Rat Peritoneal Macrophages

Abstract

To investigate the effect of noradrenaline (NE) on the immunosuppression induced by aluminum trichloride (AlCl3), the peritoneal macrophages were cultured with RPMI-1640 medium containing 0.97 mM AlCl3 (1/10 IC50). NE was added to the medium at the final concentrations of 0 (control group, N-C), 0.1 (low-dose group, N-L), 1 (mid-dose group, N-M), and 10 (high-dose group, N-H) nM, respectively. No addition of both AlCl3 and NE serviced as blank group (D-C). Chemotaxis, adhesion, phagocytosis, tumor necrosis factor α (TNF-α) secretion, cyclic adenosine monophosphate (cAMP) content, β2 adrenergic receptors (β2-AR) density, and messenger RNA (mRNA) expression of macrophages were detected. The results showed that AlCl3 reduced the chemotaxis, adhesion, phagocytosis, and TNF-α secretion and increased the cAMP content, β2-AR density, and mRNA expression of peritoneal macrophages. Meanwhile, the chemotaxis, adhesion, phagocytosis, TNF-α secretion, β2-AR density, and mRNA expression were reduced while the cAMP content was increased in NE-treated groups than those in N-C group. The results indicated that NE strengthens the immunosuppression induced by AlCl3 in cultured rat peritoneal macrophages through the β2-AR/cAMP pathway.

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Acknowledgements

The study was supported by a grant from the National Science Foundation Project (31172375, 31372496) and a grant from the National Science Foundation Project (31302147).

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Correspondence to Yanfei Li or Yanzhu Zhu.

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Li, M., Yang, X., Zhuang, C. et al. NE Strengthens the Immunosuppression Induced by AlCl3 Through β2-AR/cAMP Pathway in Cultured Rat Peritoneal Macrophages. Biol Trace Elem Res 164, 234–241 (2015). https://doi.org/10.1007/s12011-014-0217-z

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Keywords

  • NE
  • Immunosuppression
  • AlCl3
  • Peritoneal macrophages
  • β2-AR/cAMP pathway