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NE Strengthens the Immunosuppression Induced by AlCl3 Through β2-AR/cAMP Pathway in Cultured Rat Peritoneal Macrophages

Biological Trace Element Research volume 164, pages 234–241 (2015)Cite this article

Abstract

To investigate the effect of noradrenaline (NE) on the immunosuppression induced by aluminum trichloride (AlCl3), the peritoneal macrophages were cultured with RPMI-1640 medium containing 0.97 mM AlCl3 (1/10 IC50). NE was added to the medium at the final concentrations of 0 (control group, N-C), 0.1 (low-dose group, N-L), 1 (mid-dose group, N-M), and 10 (high-dose group, N-H) nM, respectively. No addition of both AlCl3 and NE serviced as blank group (D-C). Chemotaxis, adhesion, phagocytosis, tumor necrosis factor α (TNF-α) secretion, cyclic adenosine monophosphate (cAMP) content, β2 adrenergic receptors (β2-AR) density, and messenger RNA (mRNA) expression of macrophages were detected. The results showed that AlCl3 reduced the chemotaxis, adhesion, phagocytosis, and TNF-α secretion and increased the cAMP content, β2-AR density, and mRNA expression of peritoneal macrophages. Meanwhile, the chemotaxis, adhesion, phagocytosis, TNF-α secretion, β2-AR density, and mRNA expression were reduced while the cAMP content was increased in NE-treated groups than those in N-C group. The results indicated that NE strengthens the immunosuppression induced by AlCl3 in cultured rat peritoneal macrophages through the β2-AR/cAMP pathway.

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Acknowledgements

The study was supported by a grant from the National Science Foundation Project (31172375, 31372496) and a grant from the National Science Foundation Project (31302147).

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Authors and Affiliations

  1. College of Veterinary Medicine, Northeast Agricultural University, 150030, Harbin, China

    Miao Li, Xu Yang, Cuicui Zhuang, Zheng Cao, Limin Ren, Chunyu Xiu, Yanfei Li & Yanzhu Zhu

  2. Institute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, 130112, Changchun, China

    Yanzhu Zhu

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  1. Miao Li
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  2. Xu Yang
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  3. Cuicui Zhuang
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Li, M., Yang, X., Zhuang, C. et al. NE Strengthens the Immunosuppression Induced by AlCl3 Through β2-AR/cAMP Pathway in Cultured Rat Peritoneal Macrophages. Biol Trace Elem Res 164, 234–241 (2015). https://doi.org/10.1007/s12011-014-0217-z

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Keywords

  • NE
  • Immunosuppression
  • AlCl3
  • Peritoneal macrophages
  • β2-AR/cAMP pathway