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Selenium Deficiency Influences Nitric Oxide and Selenoproteins in Pancreas of Chickens

Biological Trace Element Research volume 161, pages 341–349 (2014)Cite this article

Abstract

Selenium (Se) deficiency induces pancreatic atrophy in chickens, but the molecular mechanism remains unclear. In this study, we investigated the effect of dietary Se deficiency on the expressions of 25 selenoproteins and the content of nitric oxide (NO) and examined the relationship between selenoproteins and NO. Chickens (180; 1 day old) were randomly divided into two groups, low (L) group (fed with Se deficient (Se 0.033 mg/kg) diet) and control (C) group (fed with normal (Se 0.2 mg/kg) diet). Then, pancreas was collected at 15, 25, 35, 45, and 55 days, and the content of NO, the activity of inducible NO synthase (iNOS), and the messenger RNA (mRNA) levels of 25 selenoproteins and iNOS were measured. The results showed that 25 selenoproteins were decreased (P < 0.05) by Se deficiency. Among them, thioredoxin reductase 1 (TXNRD1), selenoprotein S (SELS), selenoprotein U (SELU), selenoprotein X1 (SEPX1), and selenoprotein synthetase 2 (SPS2) were highly and extensively expressed than other types of selenoproteins in pancreas of chickens (P < 0.05). Thioredoxin reductase 2 (TXNRD2), glutathione peroxidase 1 (GPX1), glutathione peroxidase 3 (GPX3), selenoprotein I (SELI), iodothyronine deiodinase 1 (DIO1), selenoprotein P1 (SEPP1), selenoprotein W1 (SEPW1), selenoprotein O (SELO), selenoprotein T (SELT), selenoprotein M (SELM), selenoprotein X1 (SEPX1), and SPS2 were excessively decreased (P < 0.05). Meanwhile, NO content, iNOS activity, and mRNA level were increased strikingly compared with C group (P < 0.05). The correlation analysis suggested that NO had a strong negative correlation with GPX1, glutathione peroxidase 2 (GPX2), GPX3, DIO1, selenoprotein K (SELK), SELI, SEPX1, and SPS2. These results suggested that Se deficiency induced pancreatic injury by influencing NO and selenoproteins in pancreas of chickens. Thus, it offers some information on the mechanism of pancreatic injury induced by Se deficiency.

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Acknowledgments

This study was supported by the National Natural Science Foundation of China (31272626), the Study Abroad Foundation of Heilongjiang Province (LC201031), the Doctoral Fund of the Ministry of Education of China (20122325110018), and the Heilongjiang Postdoctoral Fund (LBH-Z13028).

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The authors declare that there are no conflicts of interest.

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  1. College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, People’s Republic of China

    Xia Zhao, Haidong Yao, Ruifeng Fan, Ziwei Zhang & Shiwen Xu

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  1. Xia Zhao
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  2. Haidong Yao
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  3. Ruifeng Fan
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  4. Ziwei Zhang
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  5. Shiwen Xu
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Correspondence to Ziwei Zhang or Shiwen Xu.

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Zhao, X., Yao, H., Fan, R. et al. Selenium Deficiency Influences Nitric Oxide and Selenoproteins in Pancreas of Chickens. Biol Trace Elem Res 161, 341–349 (2014). https://doi.org/10.1007/s12011-014-0139-9

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  • DOI: https://doi.org/10.1007/s12011-014-0139-9

Keywords

  • Selenium (Se) deficiency
  • Selenoproteins
  • Chickens
  • Pancreas
  • Nitric oxide (NO)