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Distribution of Graphene Oxide and TiO2-Graphene Oxide Composite in A549 Cells

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Abstract

Graphene and its derivatives are increasingly applied in nanoelectronics, biosensing, drug delivery, and biomedical applications. However, the information about its cytotoxicity remains limited. Herein, the distribution and cytotoxicity of graphene oxide (GO) and TiO2-graphene oxide composite (TiO2-GO composite) were evaluated in A549 cells. Cell viability and cell ultrastructure were measured. Our results indicated that GO could enter A549 cells and located in the cytoplasm and nucleus without causing any cell damage. TiO2 nanoparticles and GO would be separated after TiO2-GO composite entered A549 cells. TiO2-GO composite could induce cytotoxicity similar to TiO2 nanoparticles, which was probably attributed to oxidative stress. These results should be considered in the development of biological applications of GO and TiO2-GO composite.

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Acknowledgments

This work was supported by the State Key Development Program for Basic Research of China (Grant No. 2011CB932800), National Public Benefit Research Sector of China (Grant No. 201210284-02), National Natural Science Foundation of China (Grant No. 11105221), and Youth Foundation of Second Military Medical University (Grant No. 2010QN02).

Conflict of Interest Statement

The authors declare that there are no conflicts of interest.

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Authors and Affiliations

  1. Shanghai Institute of Applied Physics, Chinese Academy of Sciences, 201800, Shanghai, China

    Chan Jin, Fude Wang, Xiangzhi Zhang & Jianqiang Wang

  2. Institute of Biophysics, Second Military Medical University, 200433, Shanghai, China

    Ying Tang & Yongji Yang

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  1. Chan Jin
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  2. Fude Wang
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  3. Ying Tang
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  4. Xiangzhi Zhang
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  5. Jianqiang Wang
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Correspondence to Chan Jin.

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Jin, C., Wang, F., Tang, Y. et al. Distribution of Graphene Oxide and TiO2-Graphene Oxide Composite in A549 Cells. Biol Trace Elem Res 159, 393–398 (2014). https://doi.org/10.1007/s12011-014-0027-3

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  • DOI: https://doi.org/10.1007/s12011-014-0027-3

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