A Comparative Study on the Effects of Excess Iodine and Herbs with Excess Iodine on Thyroid Oxidative Stress in Iodine-Deficient Rats

Abstract

This study aimed to compare the effect of excess iodine and herbs with excess iodine on treating iodine deficiency-induced goiter from the perspective of oxidative stress and to measure selenium values in Chinese herbs. One hundred twenty 4-week-old Wistar rats were selected and randomly divided into four groups after inducing iodine-deficiency goiter: normal control group (NC), model control group (MC), iodine excess group (IE), and herbs with iodine excess group (HIE). The activities of oxidative enzymes and levels of oxidative products were measured using biochemical tests. The expression of 4-hydroxynonenal (4-HNE) in the thyroid was detected by immunohistochemistry and the expression of peroxiredoxin 5 (PRDX5) by the Western blot and immunohistochemistry. Selenium values in iodine-excessive herbs were measured by hydride generation-atomic fluorescence spectrometry. The herbs with iodine excess were tested to contain rich selenium. The activities of superoxide dismutase (SOD) and PRDX5 increased markedly, and the values of malondialdehyde (MDA) and 4-HNE decreased significantly in the HIE group. In conclusion, compared with excess iodine, herbs with excess iodine damaged thyroid follicular cells less, which may be related to the increase of antioxidant capacity and rich selenium values in iodine-excessive herbs.

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Acknowledgments

This work received grants from the Specialized Research Fund for the Doctoral Program of Higher Education of China (grant no. 20092133110004), Liaoning Province Education Department (grant no. 2009A492), and Talents Project of Liaoning Province Education Department (grant no. LR201026). And thanks for the support from Prof. Teng Weiping and Prof. Shan Zhongyan, Endocrinology and Metabolism Institute, China Medical University.

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Correspondence to Tianshu Gao.

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Gao, T., Shi, R., Qi, T. et al. A Comparative Study on the Effects of Excess Iodine and Herbs with Excess Iodine on Thyroid Oxidative Stress in Iodine-Deficient Rats. Biol Trace Elem Res 157, 130–137 (2014). https://doi.org/10.1007/s12011-013-9873-7

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Keywords

  • Oxidative stress
  • Goiter
  • Iodine
  • Herbs with excess iodine