A Comparative Study on the Effects of Excess Iodine and Herbs with Excess Iodine on Thyroid Oxidative Stress in Iodine-Deficient Rats
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This study aimed to compare the effect of excess iodine and herbs with excess iodine on treating iodine deficiency-induced goiter from the perspective of oxidative stress and to measure selenium values in Chinese herbs. One hundred twenty 4-week-old Wistar rats were selected and randomly divided into four groups after inducing iodine-deficiency goiter: normal control group (NC), model control group (MC), iodine excess group (IE), and herbs with iodine excess group (HIE). The activities of oxidative enzymes and levels of oxidative products were measured using biochemical tests. The expression of 4-hydroxynonenal (4-HNE) in the thyroid was detected by immunohistochemistry and the expression of peroxiredoxin 5 (PRDX5) by the Western blot and immunohistochemistry. Selenium values in iodine-excessive herbs were measured by hydride generation-atomic fluorescence spectrometry. The herbs with iodine excess were tested to contain rich selenium. The activities of superoxide dismutase (SOD) and PRDX5 increased markedly, and the values of malondialdehyde (MDA) and 4-HNE decreased significantly in the HIE group. In conclusion, compared with excess iodine, herbs with excess iodine damaged thyroid follicular cells less, which may be related to the increase of antioxidant capacity and rich selenium values in iodine-excessive herbs.
KeywordsOxidative stress Goiter Iodine Herbs with excess iodine
This work received grants from the Specialized Research Fund for the Doctoral Program of Higher Education of China (grant no. 20092133110004), Liaoning Province Education Department (grant no. 2009A492), and Talents Project of Liaoning Province Education Department (grant no. LR201026). And thanks for the support from Prof. Teng Weiping and Prof. Shan Zhongyan, Endocrinology and Metabolism Institute, China Medical University.
- 1.Gao T-S, Teng W-P (2004) Effect of mild and moderate iodine excess on thyroid function and morphology in iodine deficiency rats. Chin J Endocrinol Metab 20:353–356Google Scholar
- 2.Gao T-S, Cui P, Li H-M et al (2008) A study on the effect of Seaweed Jade Kettle Decoction on thyroid function and morphology in iodine deficiency induced goiter rats. Chin J Basic Med Tradit Chin Med 14:113–116Google Scholar
- 3.Zhang N, Tong YJ, Shan Z-Y, Teng W-P (2006) Effect of Chronic mild and moderate iodine excess on thyroid anti-oxidative ability of iodine deficiency and non-iodine deficiency Wistar rats. Natl Med J Chin 86:1274–1278Google Scholar
- 7.Cui P, Gao T-S (2007) To measure the content of iodine in herbal medicine and compound prescription used in softening hard mass and disintegrating masses. Chin Arch Tradit Chin Med 25:1396–1398Google Scholar
- 9.Gao T-S, Hu F-N, Teng W-P (2003) Effect of mild and moderate excessive iodine on thyroid function and morphology in non-iodine deficiency rats. Chin J Int Med 42:705–708Google Scholar
- 15.Yun-xia J, Rong L, Guo-qing Z et al (2010) The research status and application of organic iodine. Guangdong Trace Elem Sci 17:12–15Google Scholar