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Effect of Lower Doses of Vanadate in Combination with Azadirachta indica Leaf Extract on Hepatic and Renal Antioxidant Enzymes in Streptozotocin-Induced Diabetic Rats

Biological Trace Element Research volume 156, pages 202–209 (2013)Cite this article

Abstract

The present study was undertaken to investigate short-term (21 days) effects of oral administration of Azadirachta indica leaf extract and vanadate, separately and in combination, on the activities of antioxidant enzymes in streptozotocin-induced diabetic rats. Vanadate is a remarkable antidiabetic agent and shows insulin mimetic effect. However, severe toxicity is associated with vanadate when used in high concentration while at lower concentration the hypoglycemic property of vanadate is reduced. So, we used a low dose of vanadate in combination with A. indica leaf extract and evaluated their effect on the antioxidant defense system. Streptozotocin-diabetic rats were treated separately with insulin, vanadate (0.6 mg/ml), A. indica, and with combined dose of vanadate (0.2 mg/ml) and A. indica. At the end of the experiment, rats were sacrificed and serum glucose levels and activities of superoxide dismutase, catalase, glutathione peroxidase, and glutathione reductase were determined in cytosolic fraction of liver and kidney. Diabetic rats showed hyperglycemic condition and alteration in antioxidant enzyme activities. Treatment with antidiabetic compounds resulted in the reduction of glucose levels and restoration of enzyme activities to normal. Results showed that combined treatment of vanadate and A. indica leaf extract was the most effective in normalizing altered antioxidant enzyme system.

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Acknowledgments

Jaya Upreti acknowledges the receipt of research fellowship from University Grant Commission (UGC) during this work.

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Affiliations

  1. Department of Biosciences, Jamia Millia Islamia, Jamia Nagar, New Delhi, 110025, India

    Jaya Upreti & Seemi Farhat Basir

  2. Department of Biochemistry, Jamia Hamdard, Hamdard Nagar, New Delhi, 110062, India

    Jaya Upreti & Shakir Ali

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  1. Jaya Upreti
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  2. Shakir Ali
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  3. Seemi Farhat Basir
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Correspondence to Seemi Farhat Basir.

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Upreti, J., Ali, S. & Basir, S.F. Effect of Lower Doses of Vanadate in Combination with Azadirachta indica Leaf Extract on Hepatic and Renal Antioxidant Enzymes in Streptozotocin-Induced Diabetic Rats. Biol Trace Elem Res 156, 202–209 (2013). https://doi.org/10.1007/s12011-013-9827-0

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Keywords

  • Sodium Orthovanadate
  • Azadirachta indica
  • Streptozotocin Diabetes
  • Oxidative Stress
  • Antioxidant