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Biological Trace Element Research

, Volume 155, Issue 2, pp 247–252 | Cite as

Effects of High Dietary Fluorine on Erythrocytes and Erythrocyte Immune Adherence Function in Broiler Chickens

  • Yubing Deng
  • Hengmin CuiEmail author
  • Xi Peng
  • Jing Fang
  • Zhicai Zuo
  • Junliang Deng
  • Qin Luo
Article

Abstract

Fluoride can exert toxic effects on soft tissues, giving rise to a broad array of symptoms and pathological changes. The aim of this study was to investigate on erythrocytes and erythrocyte immune adherence function in broiler chickens fed with high fluorine (F) diets by measuring the total erythrocyte count (TEC), the contents of hemoglobin (Hb), packed cell volumn (PCV), erythrocyte osmotic fragility (EOF), erythrocyte C3b receptor rosette rate (E-C3bRR), and erythrocyte immune complex rosette rate (E-ICRR). A total of 280 1-day-old healthy avian broiler chickens were randomly allotted into four equal groups of 70 birds each and fed with a corn–soybean basal diet containing 22.6 mg F/kg (control group) or same basal diets supplemented with 400, 800, and 1,200 mg F/kg (high F groups I, II, and III) in the form of sodium fluoride for 42 days. Blood samples were collected for the abovementioned parameters analysis at 14, 28, and 42 days of age during the experiment. The experimental results indicated that TEC, Hb, and PCV were significantly lower (p < 0.05 or p < 0.01), and EOF was higher (p < 0.05 or p < 0.01) in the high F groups II and III than that in the control group from 14 to 42 days of age. The E-C3bRR was significantly decreased (p < 0.01) in the three high F groups, whereas the E-ICRR was markedly increased (p < 0.01) in the high F groups II and III from 14 to 42 days of age. It was concluded that dietary F in the range of 800 to 1, 200 mg/kg could significantly cause anemia and impair the integrity of erythrocyte membrane, the transport capacity of oxygen and carbon dioxide, and erythrocyte immune adherence function in broiler chickens.

Keywords

Broiler chicken EOF Erythrocyte immune adherence function Hb High dietary fluorine PCV TEC 

Notes

Acknowledgments

This research was supported by the program for Changjiang Scholars and the University Innovative Research Team (IRT 0848), and the Education Department of Sichuan Province (09ZZ017).

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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Yubing Deng
    • 1
  • Hengmin Cui
    • 1
    Email author
  • Xi Peng
    • 1
  • Jing Fang
    • 1
  • Zhicai Zuo
    • 1
  • Junliang Deng
    • 1
  • Qin Luo
    • 1
  1. 1.Key Laboratory of Animal Diseases and Environmental Hazards of Sichuan Province, College of Veterinary MedicineSichuan Agricultural UniversityYa’anChina

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