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Phenylethynyl-Butyltellurium Inhibits the Sulfhydryl Enzyme Na+, K+-ATPase: An Effect Dependent on the Tellurium Atom

Biological Trace Element Research volume 155, pages 261–266 (2013)Cite this article

Abstract

Organotellurium compounds are known for their toxicological effects. These effects may be associated with the chemical structure of these compounds and the oxidation state of the tellurium atom. In this context, 2-phenylethynyl-butyltellurium (PEBT) inhibits the activity of the sulfhydryl enzyme, δ-aminolevulinate dehydratase. The present study investigated on the importance of the tellurium atom in the PEBT ability to oxidize mono- and dithiols of low molecular weight and sulfhydryl enzymes in vitro. PEBT, at high micromolar concentrations, oxidized dithiothreitol (DTT) and inhibited cerebral Na+, K+-ATPase activity, but did not alter the lactate dehydrogenase activity. The inhibition of cerebral Na+, K+-ATPase activity was completely restored by DTT. By contrast, 2-phenylethynyl-butyl, a molecule without the tellurium atom, neither oxidized DTT nor altered the Na+, K+-ATPase activity. In conclusion, the tellurium atom of PEBT is crucial for the catalytic oxidation of sulfhydryl groups from thiols of low molecular weight and from Na+, K+-ATPase.

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Acknowledgments

The financial support by UFSM, CAPES, FAPERGS/CNPq (PRONEX) (research grant no. 10/0005-1) and FAPERGS (no. 10/0711-6) is gratefully acknowledged. C.W.N is a recipient of CNPq fellowship.

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Affiliations

  1. Laboratório de Síntese, Reatividade e Avaliação Farmacológica e Toxicológica de Organocalcogênios, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Santa Maria, CEP, 97105-900, RS, Brazil

    Caroline B. Quines, Suzan G. Rosa, José S. S. Neto, Gilson Zeni & Cristina W. Nogueira

  2. Departamento de Química, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Santa Maria, 97105-900, RS, Brazil

    Cristina W. Nogueira

Authors
  1. Caroline B. Quines
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  2. Suzan G. Rosa
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  3. José S. S. Neto
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  4. Gilson Zeni
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  5. Cristina W. Nogueira
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Correspondence to Cristina W. Nogueira.

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Quines, C.B., Rosa, S.G., Neto, J.S.S. et al. Phenylethynyl-Butyltellurium Inhibits the Sulfhydryl Enzyme Na+, K+-ATPase: An Effect Dependent on the Tellurium Atom. Biol Trace Elem Res 155, 261–266 (2013). https://doi.org/10.1007/s12011-013-9781-x

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  • DOI: https://doi.org/10.1007/s12011-013-9781-x

Keywords

  • Tellurium
  • Sulfhydryl enzymes
  • Thiol
  • Toxicity
  • Na+, K+-ATPase