Biological Trace Element Research

, Volume 155, Issue 1, pp 114–118 | Cite as

Correlation Between Endodontic Broken Instrument and Nickel Level in Urine

  • Mohammad Ali SaghiriEmail author
  • Nader Sheibani
  • Franklin Garcia-Godoy
  • Armen Asatourian
  • Peiman Mehriar
  • Mark Scarbecz


This study aims to evaluate the correlation between the presences of separated endodontic instrument inside the dental canal and the nickel (Ni) level in the urine samples of subjected patients. Same-gendered and near-aged participants were selected and were instructed to collect their urine in sterile nickel-free plastic containers. The procedures were carried out in the office, and samples were stored in low-temperature cooler for 1 day and then they were transferred to the laboratory for electrothermal atomic absorption spectrometry. The level of Ni was measured and the correlation coefficient was calculated. Data were analyzed using t tests, Pearson’s correlation coefficients, and linear regression analysis, at a level of significance P < 0.05. The statistical analysis has showed significant difference in Ni level between endodontic and control groups (P < 0.05). There was no correlation between Ni level in urine and the age or time period of broken instrument inside the canal; however, Ni level of urine and the age of participants in experimental group has demonstrated a positive correlation. The amount of Ni element can be increased in the urine of patients who have experienced broken endodontic instrument inside the dental canal. However, there is no positive correlation between the remaining pieces of instruments inside the canal and the elevation of nickel amount in urine during the tested time period. This issue suggested that the aging of remaining broken instrument inside the canal does not show any remarkable concern regarding the Ni elevation in the urine excreted by an individual.


Allergen Broken instrument Fatigue fracture NiTi rotary instrument Nickel toxicity 



We are indebted to the late Professor Kamal Asgar for the provision of laboratory facilities. Also, special thanks go to Professor Hajar Afsar Ladjvardi, Dr. Amir Nazari and Ms. Neda Bayati for all of their contributions to this research.

Conflict of Interest

The authors affirm that they have no financial affiliation or involvement with any commercial organization with direct financial interest in the subject or materials discussed in this manuscript and deny any conflicts of interest related to this study.


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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Mohammad Ali Saghiri
    • 1
    • 2
    Email author
  • Nader Sheibani
    • 1
  • Franklin Garcia-Godoy
    • 3
  • Armen Asatourian
    • 2
  • Peiman Mehriar
    • 2
  • Mark Scarbecz
    • 3
  1. 1.Department of Ophthalmology and Visual SciencesUniversity of Wisconsin, school of Medicine and Public HealthMadisonUSA
  2. 2.Kamal Asgar Research CenterEncinoUSA
  3. 3.Department of Bioscience ResearchUniversity of Tennessee Health Science CenterMemphisUSA

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