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Biological Trace Element Research

, Volume 154, Issue 2, pp 196–201 | Cite as

Humoral and Cellular Immunity in Chromium Picolinate-Supplemented Lambs

  • B. S. L. DallagoEmail author
  • C. M. McManus
  • D. F. Caldeira
  • A. Campeche
  • R. T. Burtet
  • T. P. Paim
  • E. F. Gomes
  • R. P. Branquinho
  • S. V. Braz
  • H. Louvandini
Article

Abstract

The effects of oral supplementation of chromium picolinate (CrPic) on humoral and cellular immunity in sheep were investigated. Twenty-four male lambs divided into four treatments and received different dosages of CrPic: placebo (0), 0.250, 0.375, and 0.500 mg of chromium/animal/day during 84 days. The base ration was Panicum maximum cv Massai hay and concentrate. Blood samples were collected fortnightly for total and differential leukocyte counts. On days 28 and 56, the lambs were challenged with chicken ovalbumin I.M. Serum samples were collected on days 46 and 74 and subjected to an indirect enzyme-linked immunosorbent assay to measure IgG anti-ovalbumin. The cell-mediated immune response was determined by a delay-type hypersensitivity test using phytohemagglutinin. CrPic did not significantly affect humoral immunity in lambs but there was a negative effect on cellular immunity (P < 0.05) as Cr supplementation increased. Therefore, the level of Cr supplementation for lambs must be better studied to address its effect on stressed animals or the possible toxic effects of Cr on the animal itself or its immune system.

Keywords

Hair sheep Delay-type hypersensibility Micromineral supplementation Cell-mediated immune response Superfluous cost 

Notes

Acknowledgments

The authors would like to thank CNPq, INCT-Pecuária (CNPq/FAPEMIG), and CAPES/PROCAD-“Novas Fronteiras 2007” for the scholarships and to FINATEC and FAPDF for financial support. Special thanks to the teachers Márcio Martins Pimentel and Andrea Queiroz Maranhão for their technical support of this study.

References

  1. 1.
    Moonsie-Shageer S, Mowat DN (1993) Effect of level of supplemental chromium on performance, serum constituents, and immune status of stressed feeder calves. J Anim Sci 71:232–238PubMedGoogle Scholar
  2. 2.
    Chang X, Mallard BA, Mowat DN (1996) Effects of chromium on health status, blood neutrophil phagocytosis and in vitro lymphocyte blastogenesis of dairy cows. Vet Immunol Immunopathol 52:37–52PubMedCrossRefGoogle Scholar
  3. 3.
    Spears JW (2000) Micronutrients and immune function in cattle. Proc Nutr Soc 59:587–594PubMedCrossRefGoogle Scholar
  4. 4.
    Arthington JD, Corah LR, Minton JE (1997) Supplemental dietary chromium does not influence ACTH, cortisol, or immune responses in young calves inoculated with bovine herpevirus-1. J Anim Sci 75:217–223PubMedGoogle Scholar
  5. 5.
    Gentry LR, Fernandez JM, Ward T, White TW, Southern LL, Bidner TD, Thompson DL, Horohov DW, Chapa AM, Sahlu T (1999) Dietary protein and chromium tripicolinate in suffolk wether lambs: effects on production characteristics, metabolic and hormonal responses, and immune status. J Anim Sci 77:1284–1294PubMedGoogle Scholar
  6. 6.
    Chang X, Mowat DN (1992) Supplemental chromium for stressed and growing feeder calves. J Anim Sci 70:559–565PubMedGoogle Scholar
  7. 7.
    Kegley EB, Spears JW, Brown TT Jr (1997) Effect of shipping and chromium supplementation on performance, immune response, and disease resistence of steers. J Anim Sci 75:1956–1964PubMedGoogle Scholar
  8. 8.
    Striffler JS, Polansky MM, Anderson RA (1993) Dietary chromium enhances insulin secretion in perfused rat pancreas. J Trace Elem Med Biol 6:75–81Google Scholar
  9. 9.
    Hodgson E, Cope WG, Leidy RB (2004) Classes of toxicants: use classes. In: Hodgson E (ed) A textbook of modern toxicology. Wiley, New Jersey, pp 49–74CrossRefGoogle Scholar
  10. 10.
    Chaturvedi UC, Shrivastava RK, Upreti RK, Seth PK (2002) Effects of chromium on the immune system. FEMS Immunol Med Microbiol 34:1–7PubMedCrossRefGoogle Scholar
  11. 11.
    Tizard IR (2008) Imunologia veterinária, 8th edn. Elsevier, Rio de JaneiroGoogle Scholar
  12. 12.
    Kegley EB, Spears JW (1995) Immune response, glucose metabolism, and performance of stressed feeder calves fed inorganic or organic chromium. J Anim Sci 73:2721–2726PubMedGoogle Scholar
  13. 13.
    Smits JE, Bortolotti GR, Tella JL (1999) Simplifying the phytohaemagglutinin skin-testing technique in studies of avian immunocompetence. Funct Ecol 13:567–572CrossRefGoogle Scholar
  14. 14.
    Minton JE, Reddy PG, Blecha F (1991) Removal of nocturnal secretion of melatonin fails to reduce antibody synthesis and interleukin-2 production of lambs. J Anim Sci 69:565–570PubMedGoogle Scholar
  15. 15.
    Dallago BSL, McManus C, Caldeira DF, Lopes AC, Paim TP, Franco E, Borges BO, Teles PHF, Correa PS, Louvandini H (2011) Performance and ruminal protozoa in lambs with chromium supplementation. Res Vet Sci 90:253–256PubMedCrossRefGoogle Scholar
  16. 16.
    Silva DJ, Queiroz AC (2006) Análise de alimentos: métodos químicos e biológicos, 3rd edn. UFV, ViçosaGoogle Scholar
  17. 17.
    César MC, Zanetti MA, Salles MSV, Brisola ML (2003) Desempenho e resposta metabólica de bezerros recebendo dietas suplementadas com cromo. Rev Bras Zootec 32:1532–1535CrossRefGoogle Scholar
  18. 18.
    Mostafa-Tehrani A, Ghorbani G, Zare-Shahneh A, Mirhadi SA (2006) Non-carcass components and wholesale cuts of Iranian fat-tailed lambs fed chromium nicotinate or chromium chloride. Small Rumin Res 63:12–19CrossRefGoogle Scholar
  19. 19.
    Pechova A, Pavlata L (2007) Chromium as an essential nutrient: a review. Vet Med 52:1–18Google Scholar
  20. 20.
    Levina A, Lay PA (2008) Chemical properties and toxicity of chromium (III) nutritional supplements. Chem Res Toxicol 21:563–571PubMedCrossRefGoogle Scholar
  21. 21.
    Burton JL, Mallard BA, Nonnecke BJ, Dubeski PL, Elsasser TH (1996) Effects of supplemental chromium on production of cytokines by mitogen-stimulated bovine peripheral blood mononuclear cells. J Dairy Sci 79:2237–2246PubMedCrossRefGoogle Scholar
  22. 22.
    Mallard BA, Borgs P, Ireland MJ, McBride BW, Brown BD, Irwin JA (1999) Immunomodulatory effects of chromium (III) in ruminants: a review of potential health benefits and effects on production and milk quality. J Trace Elem Exp Med 12:131–140CrossRefGoogle Scholar
  23. 23.
    Abbas AK, Lichtman AH (2005) Ativação de Linfócitos T. In: Abbas AK, Lichtman AH (eds) Imunologia celular e molecular. Elsevier, Rio de Janeiro, pp 169–195Google Scholar
  24. 24.
    Burton JL, Mallard BA, Mowat DN (1993) Effects of supplemental chromium on immune responses of periparturient and early lactation dairy cows. J Anim Sci 71:1532–1539PubMedGoogle Scholar
  25. 25.
    Kegley EB, Spears JW, Brown TT Jr (1996) Immune response and disease resistance of calves fed chromium nicotinic acid complex or chromium chloride. J Dairy Sci 79:1278–1283PubMedCrossRefGoogle Scholar
  26. 26.
    Lien TF, Yang KH, Link KJ (2005) Effects of chromium propionate supplementation on growth performance, serum traits and immune response in weaned pigs. Asian Aust J Anim Sci 18:403–408Google Scholar

Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • B. S. L. Dallago
    • 1
    • 4
    Email author
  • C. M. McManus
    • 1
  • D. F. Caldeira
    • 1
  • A. Campeche
    • 1
  • R. T. Burtet
    • 2
  • T. P. Paim
    • 3
  • E. F. Gomes
    • 3
  • R. P. Branquinho
    • 1
  • S. V. Braz
    • 1
  • H. Louvandini
    • 1
    • 3
  1. 1.Faculdade de Agronomia e Medicina VeterináriaUniversidade de BrasíliaBrasíliaBrazil
  2. 2.Laboratório de Biologia MolecularUniversidade de BrasíliaBrasíliaBrazil
  3. 3.Centro de Energia Nuclear na AgriculturaPiracicabaBrazil
  4. 4.Universidade de BrasíliaBrasíliaBrazil

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