Biological Trace Element Research

, Volume 154, Issue 2, pp 304–311 | Cite as

Preparation of Two Organoselenium Compounds and Their Induction of Apoptosis to SMMC-7221 Cells

  • Pei Guo
  • Qing Wang
  • Jing Liu
  • Lina Liu
  • Peiguang Zhao
  • Yu CaoEmail author
  • Yuping Liu
  • Chao QiEmail author
  • Yanli Liu


Two organoselenium compounds: xylitol selenious ester (xylitol-Se) and sucrose selenious ester (sucrose-Se) were synthesized, and their molecular structures were characterized in this study. In MTT assay, xylitol-Se and sucrose-Se showed cytostatic effects on human hepatocellular carcinoma cells SMMC-7221 in a dose-dependent manner, whereas they had no negative influences on the proliferation of human normal hepatic cells HL-7702 in the concentration range from 0.15 to 1.2 ppm Se. Morphological observation, agarose gel electrophoresis, and caspase-3 assay indicated that xylitol-Se and sucrose-Se induced mitochondrial apoptosis to SMMC-7221 cells, which is supported by the depletion of mitochondrial membrane potential and suppression of caspase-3 activity, indicating their ability of inducing apoptosis to cancer cells and great potentials as anticancer drugs.

Key words

Xylitol selenious ester Sucrose selenious ester Anticancer Mitochondrial apoptosis SMMC-72 cells 



This work was supported by the Scientific Research Foundation for the Returned Overseas Chinese Scholars (State Education Ministry, 2010-1174), self-determined research funds of CCNU from the colleges' basic research and operation of MOE (CCNU11A02013), and the project of Hubei Key Laboratory of Genetic Regulation and Integrative Biology (201002).

Integrity of research and reporting

Ethical standards All human and animal studies have been approved by the appropriate ethics committee and have therefore been performed in accordance with the ethical standards laid down in the 1964 Declaration of Helsinki and its later amendments. All persons gave their informed consent prior to their inclusion in the study.

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

12011_2013_9715_MOESM1_ESM.docx (481 kb)
ESM 1 (DOCX 480 kb)


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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Pei Guo
    • 1
  • Qing Wang
    • 1
  • Jing Liu
    • 2
  • Lina Liu
    • 1
  • Peiguang Zhao
    • 1
  • Yu Cao
    • 1
    • 2
    Email author
  • Yuping Liu
    • 1
  • Chao Qi
    • 2
    Email author
  • Yanli Liu
    • 2
  1. 1.Key Laboratory of Pesticide and Chemical Biology (Ministry of Education), College of ChemistryCentral China Normal UniversityWuhanPeople’s Republic of China
  2. 2.Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life ScienceCentral China Normal UniversityWuhanPeople’s Republic of China

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