Preparation of Two Organoselenium Compounds and Their Induction of Apoptosis to SMMC-7221 Cells
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Two organoselenium compounds: xylitol selenious ester (xylitol-Se) and sucrose selenious ester (sucrose-Se) were synthesized, and their molecular structures were characterized in this study. In MTT assay, xylitol-Se and sucrose-Se showed cytostatic effects on human hepatocellular carcinoma cells SMMC-7221 in a dose-dependent manner, whereas they had no negative influences on the proliferation of human normal hepatic cells HL-7702 in the concentration range from 0.15 to 1.2 ppm Se. Morphological observation, agarose gel electrophoresis, and caspase-3 assay indicated that xylitol-Se and sucrose-Se induced mitochondrial apoptosis to SMMC-7221 cells, which is supported by the depletion of mitochondrial membrane potential and suppression of caspase-3 activity, indicating their ability of inducing apoptosis to cancer cells and great potentials as anticancer drugs.
Key wordsXylitol selenious ester Sucrose selenious ester Anticancer Mitochondrial apoptosis SMMC-72 cells
This work was supported by the Scientific Research Foundation for the Returned Overseas Chinese Scholars (State Education Ministry, 2010-1174), self-determined research funds of CCNU from the colleges' basic research and operation of MOE (CCNU11A02013), and the project of Hubei Key Laboratory of Genetic Regulation and Integrative Biology (201002).
Integrity of research and reporting
Ethical standards All human and animal studies have been approved by the appropriate ethics committee and have therefore been performed in accordance with the ethical standards laid down in the 1964 Declaration of Helsinki and its later amendments. All persons gave their informed consent prior to their inclusion in the study.
Conflict of interest
The authors declare that they have no conflict of interest.
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