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Influence of Selenium on Mast Cell Mediator Release

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Abstract

Selenium supplementation still enhanced the immune response even in individuals who, according to current standards, would be considered as not being overtly selenium deficient. Mast cells are granulated cells that play a pivotal role in allergic reactions. In this study, we investigated the modulatory effect of sodium selenite on mediator release and degranulation of murine mast cell line (MC/9). Cells were pre-treated with selenium selenite (1, 2, 3 μg/ml) for 24 h and controls left untreated. Then, cells were sensitized overnight with anti-dinitrophenyl (DNP) IgE and challenged with DNP/HSA for degranulation induction. The histamine and prostaglandin D2 (PGD2) were measured by ELISA, and β-hexosaminidase was measured by spectrophotometery method. Selenium-treated cells revealed significant decrease in concentration of PGD2 (P = 0.019) and β-hexosaminidase (P = 0.009). In addition, a slight reduction of histamine release by the selenium-treated cells was observed, based on our intracellular and extracellular assessments. The most inhibitory effect of selenium supplementation on mediator release of MC/9 cells was obtained in the presence of 3 μg/ml of sodium selenite. The results of the present study demonstrate beneficial effects of supplemental selenium in attenuating clinical manifestations of allergy and asthma.

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Acknowledgments

This research has been supported by a grant from the Tehran University of Medical Sciences (grant code 88-04-40-10238).

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Authors and Affiliations

  1. Department of Animal Biology, Faculty of Natural Science, University of Tabriz, 29 bahman Blvd, Tabriz, Iran

    Reza Safaralizadeh

  2. Immunology Asthma and Allergy Research Institute, Tehran University of Medical Sciences, Tehran, Iran

    Maryam Nourizadeh, Ahad Zare, Gholam Ali Kardar & Zahra Pourpak

  3. Departmen of Immunology and Allergy, Children Medical Center, Tehran University of Medical Sciences, Tehran, Iran

    Zahra Pourpak

Authors
  1. Reza Safaralizadeh
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  2. Maryam Nourizadeh
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  3. Ahad Zare
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  4. Gholam Ali Kardar
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  5. Zahra Pourpak
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Correspondence to Zahra Pourpak.

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Safaralizadeh, R., Nourizadeh, M., Zare, A. et al. Influence of Selenium on Mast Cell Mediator Release. Biol Trace Elem Res 154, 299–303 (2013). https://doi.org/10.1007/s12011-013-9712-x

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  • DOI: https://doi.org/10.1007/s12011-013-9712-x

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