Advertisement

Biological Trace Element Research

, Volume 154, Issue 2, pp 299–303 | Cite as

Influence of Selenium on Mast Cell Mediator Release

  • Reza Safaralizadeh
  • Maryam Nourizadeh
  • Ahad Zare
  • Gholam Ali Kardar
  • Zahra PourpakEmail author
Article

Abstract

Selenium supplementation still enhanced the immune response even in individuals who, according to current standards, would be considered as not being overtly selenium deficient. Mast cells are granulated cells that play a pivotal role in allergic reactions. In this study, we investigated the modulatory effect of sodium selenite on mediator release and degranulation of murine mast cell line (MC/9). Cells were pre-treated with selenium selenite (1, 2, 3 μg/ml) for 24 h and controls left untreated. Then, cells were sensitized overnight with anti-dinitrophenyl (DNP) IgE and challenged with DNP/HSA for degranulation induction. The histamine and prostaglandin D2 (PGD2) were measured by ELISA, and β-hexosaminidase was measured by spectrophotometery method. Selenium-treated cells revealed significant decrease in concentration of PGD2 (P = 0.019) and β-hexosaminidase (P = 0.009). In addition, a slight reduction of histamine release by the selenium-treated cells was observed, based on our intracellular and extracellular assessments. The most inhibitory effect of selenium supplementation on mediator release of MC/9 cells was obtained in the presence of 3 μg/ml of sodium selenite. The results of the present study demonstrate beneficial effects of supplemental selenium in attenuating clinical manifestations of allergy and asthma.

Keywords

Murine mast cell line Selenium Histamine Prostaglandin D2 β-Hexosaminidase 

Notes

Acknowledgments

This research has been supported by a grant from the Tehran University of Medical Sciences (grant code 88-04-40-10238).

References

  1. 1.
    Kim SH, Johnson VJ, Shin TY, Sharma RP (2004) Selenium attenuates lipopolysaccharide-induced oxidative stress responses through modulation of p38 MAPK and NF-kappaB signaling pathways. Exp Biol Med (Maywood) 229(2):203–213Google Scholar
  2. 2.
    Broome CS, McArdle F, Kyle JA, Andrews F, Lowe NM, Hart CA, Arthur JR, Jackson MJ (2004) An increase in selenium intake improves immune function and poliovirus handling in adults with marginal selenium status. Am J Clin Nutr 80(1):154–162PubMedGoogle Scholar
  3. 3.
    Thomson CD, Wickens K, Miller J, Ingham T, Lampshire P, Epton MJ, Town GI, Pattemore P, Crane J (2012) Selenium status and allergic disease in a cohort of New Zealand children. Clin Exp Allergy 42(4):560–567. doi: 10.1111/j.1365-2222.2012.03924.x PubMedCrossRefGoogle Scholar
  4. 4.
    Matsui T, Suzuki Y, Yamashita K, Yoshimaru T, Suzuki-Karasaki M, Hayakawa S, Yamaki M, Shimizu K (2000) Diphenyleneiodonium prevents reactive oxygen species generation, tyrosine phosphorylation, and histamine release in RBL-2H3 mast cells. Biochem Biophys Res Commun 276(2):742–748. doi: 10.1006/bbrc.2000.3545 PubMedCrossRefGoogle Scholar
  5. 5.
    Amin K (2012) The role of mast cells in allergic inflammation. Respir Med 106(1):9–14. doi: 10.1016/j.rmed.2011.09.007 PubMedCrossRefGoogle Scholar
  6. 6.
    Kadrabova J, Mad’aric A, Kovacikova Z, Podivinsky F, Ginter E, Gazdik F (1996) Selenium status is decreased in patients with intrinsic asthma. Biol Trace Elem Res 52(3):241–248. doi: 10.1007/BF02789165 PubMedCrossRefGoogle Scholar
  7. 7.
    Gueck T, Aschenbach JR, Fuhrmann H (2002) Influence of vitamin E on mast cell mediator release. Vet Dermatol 13(6):301–305PubMedCrossRefGoogle Scholar
  8. 8.
    Guo CH, Liu PJ, Hsia S, Chuang CJ, Chen PC (2011) Role of certain trace minerals in oxidative stress, inflammation, CD4/CD8 lymphocyte ratios and lung function in asthmatic patients. Ann Clin Biochem 48(Pt 4):344–351. doi: 10.1258/acb.2011.010266 PubMedCrossRefGoogle Scholar
  9. 9.
    Allan K, Devereux G (2011) Diet and asthma: nutrition implications from prevention to treatment. J Am Diet Assoc 111(2):258–268. doi: 10.1016/j.jada.2010.10.048 PubMedCrossRefGoogle Scholar
  10. 10.
    Huang Z, Rose AH, Hoffmann PR (2012) The role of selenium in inflammation and immunity: from molecular mechanisms to therapeutic opportunities. Antioxid Redox Signal 16(7):705–743. doi: 10.1089/ars.2011.4145 PubMedCrossRefGoogle Scholar
  11. 11.
    Vunta H, Belda BJ, Arner RJ, Channa Reddy C, Vanden Heuvel JP, Sandeep Prabhu K (2008) Selenium attenuates pro-inflammatory gene expression in macrophages. Mol Nutr Food Res 52(11):1316–1323. doi: 10.1002/mnfr.200700346 PubMedCrossRefGoogle Scholar
  12. 12.
    Brooks AC, Whelan CJ, Purcell WM (1999) Reactive oxygen species generation and histamine release by activated mast cells: modulation by nitric oxide synthase inhibition. Br J Pharmacol 128(3):585–590. doi: 10.1038/sj.bjp.0702838 PubMedCrossRefGoogle Scholar
  13. 13.
    Safaralizadeh R, Soheili ZS, Deezagi A, Pourpak Z, Samiei S, Moin M (2009) FcepsilonRI-alpha siRNA inhibits the antigen-induced activation of mast cells. Iran J Allergy Asthma Immunol 8(4):177–183PubMedGoogle Scholar
  14. 14.
    Hoffmann PR (2012) Asthma in children and nutritional selenium get another look. Clin Exp Allergy 42(4):488–489. doi: 10.1111/j.1365-2222.2011.03949.x PubMedCrossRefGoogle Scholar
  15. 15.
    Carneiro MF, Rhoden CR, Amantea SL, Barbosa F Jr (2011) Low concentrations of selenium and zinc in nails are associated with childhood asthma. Biol Trace Elem Res 144(1–3):244–252. doi: 10.1007/s12011-011-9080-3 PubMedCrossRefGoogle Scholar
  16. 16.
    Wintergerst ES, Maggini S, Hornig DH (2007) Contribution of selected vitamins and trace elements to immune function. Ann Nutr Metab 51(4):301–323. doi: 10.1159/000107673 PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Reza Safaralizadeh
    • 1
  • Maryam Nourizadeh
    • 2
  • Ahad Zare
    • 2
  • Gholam Ali Kardar
    • 2
  • Zahra Pourpak
    • 2
    • 3
    Email author
  1. 1.Department of Animal Biology, Faculty of Natural ScienceUniversity of TabrizTabrizIran
  2. 2.Immunology Asthma and Allergy Research InstituteTehran University of Medical SciencesTehranIran
  3. 3.Departmen of Immunology and Allergy, Children Medical CenterTehran University of Medical SciencesTehranIran

Personalised recommendations