Biological Trace Element Research

, Volume 151, Issue 2, pp 171–180 | Cite as

Toxicological Status of Children with Autism vs. Neurotypical Children and the Association with Autism Severity

  • James B. Adams
  • Tapan Audhya
  • Sharon McDonough-Means
  • Robert A. Rubin
  • David Quig
  • Elizabeth Geis
  • Eva Gehn
  • Melissa Loresto
  • Jessica Mitchell
  • Sharon Atwood
  • Suzanne Barnhouse
  • Wondra Lee
Article

Abstract

This study investigates both the level of toxic metals in children with autism and the possible association of those toxic metals with autism severity. This study involved 55 children with autism ages 5–16 years compared to 44 controls with similar age and gender. The study included measurements of toxic metals in whole blood, red blood cells (RBC), and urine. The autism group had higher levels of lead in RBC (+41 %, p = 0.002) and higher urinary levels of lead (+74 %, p = 0.02), thallium (+77 %, p = 0.0001), tin (+115 %, p = 0.01), and tungsten (+44 %, p = 0.00005). However, the autism group had slightly lower levels of cadmium in whole blood (−19 %, p = 0.003). A stepwise, multiple linear regression analysis found a strong association of levels of toxic metals with variation in the degree of severity of autism for all the severity scales (adjusted R2 of 0.38–0.47, p < 0.0003). Cadmium (whole blood) and mercury (whole blood and RBC) were the most consistently significant variables. Overall, children with autism have higher average levels of several toxic metals, and levels of several toxic metals are strongly associated with variations in the severity of autism for all three of the autism severity scales investigated.

Keywords

Autism Toxic metals Mercury Lead Thallium Tungsten 

References

  1. 1.
    James SJ, Cutler P, Melnyk S, Jernigan S, Janak L, Gaylor DW, Neubrander JA (2004) Metabolic biomarkers of increased oxidative stress and impaired methylation capacity in children with autism. Am J Clin Nutr 80(6):1611–1617PubMedGoogle Scholar
  2. 2.
    James SJ, Melnyk S, Jernigan S, Cleves MA, Halsted CH, Wong DH, Cutler P, Bock K, Boris M, Bradstreet JJ, Baker SM, Gaylor DW (2006) Metabolic endophenotype and related genotypes are associated with oxidative stress in children with autism. Am J Med Genet B Neuropsychiatr Genet 141:947–956Google Scholar
  3. 3.
    James SJ, Melnyk S, Fuchs G, Reid T, Jernigan S, Pavliv O, Hubanks A, Gaylor DW (2009) Efficacy of methylcobalamin and folinic acid treatment on glutathione redox status in children with autism. Am J Clin Nutr 89((1):425–430, Epub 2008 Dec 3Google Scholar
  4. 4.
    Adams JB, Audhya T, Mcdonough-Means S, Rubin RA, Quig D, Geis E, Gehn E, Loresto M, Mitchell J, Atwood S, Barnhouse S, Lee W (2011) Nutritional and metabolic status of children with autism vs. neurotypical children, and the association with autism severity. Nutr Metab (Lond) 8(1):34, Jun 8CrossRefGoogle Scholar
  5. 5.
    Konstantareas MM, Homatidis S (1987) Ear infections in autistic and normal children. J Autism Dev Disord 17:585–594PubMedCrossRefGoogle Scholar
  6. 6.
    Adams JB, Holloway CE, Margolis M, George F (2003) Heavy metal exposures, developmental milestones, and physical symptoms in children with autism. Conference Proceedings of the Fall 2003 Defeat Autism Now! Conference on Oct 3–5, 2003 in Portland, Oregon, p. 71–75.Google Scholar
  7. 7.
    Adams JB, Romdalvik J, Ramanujam VMS, Legator MS (2007) Mercury, lead, and zinc in baby teeth of children with autism vs. controls. J Toxicology Environ Health A 70:1046–1051CrossRefGoogle Scholar
  8. 8.
    Adams JB, Romdalvik J, Levine KE, Hu L-W (2008) Mercury in first-cut baby hair of children with autism vs. typically-developing children. Toxicol Environ Chem 90(4):739–753CrossRefGoogle Scholar
  9. 9.
    Rowland IR, Davies M, Evans J (1980) Tissue content of mercury in rats given methylmercury chloride orally: influence of intestinal flora. Arch Environ Health 35:155–160PubMedGoogle Scholar
  10. 10.
    Rowland IR, Robinson RD, Doherty RA (1984) Effect of diet on mercury metabolism and excretion in mice given methylmercury: role of gut flora. Arch Env Health 39:401–408CrossRefGoogle Scholar
  11. 11.
    Holmes AS, Blaxill MF, Haley BE (2003) Reduced levels of mercury in first baby haircuts of autistic children. Int J Toxicology 22(4):277–285CrossRefGoogle Scholar
  12. 12.
    Kern JK, Grannemann BD, Trivedi MH (2007) Adams J.B. sulfhydryl-reactive metals in autism. J Toxicol Environ Health A 70:1–7CrossRefGoogle Scholar
  13. 13.
    Ip P, Wong V, Ho M, Lee J, Wong W (2004) Mercury exposure in children with autistic spectrum disorder: case–control study. J Child Neurol 19:431–434PubMedGoogle Scholar
  14. 14.
    DeSoto MC, Hitlan RT (2007) Blood levels of mercury are related to diagnosis of autism: a reanalysis of an important data set. J Child Neurology 22:1308–1311CrossRefGoogle Scholar
  15. 15.
    Fido A, Al-Saad S (2005) Toxic trace elements in the hair of children with autism. Autism 9(3):290–298PubMedCrossRefGoogle Scholar
  16. 16.
    Abdullah MM, Ly AR, Goldberg WA, Clarke-Stewart KA, Dudgeon JV, Mull CG, Chan TJ, Kent EE, Mason AZ, Ericson JE (2012) Heavy Metal in Children’s Tooth Enamel: Related to Autism and Disruptive Behaviors? J Autism Dev Disord 42:929–936PubMedCrossRefGoogle Scholar
  17. 17.
    Ash MM, Nelson SJ (2003) Wheeler’s dental anatomy, physiology and occlusion, 8th edn. Elsevier, St LouisGoogle Scholar
  18. 18.
    Farmer JG, MacKenzie AB, Moody GH (2006) Human teeth as historical biomonitors of environmental and dietary lead: some lessons from isotopic studies of 19th and 20th century archival material. Environ Geochem Heal 28:421–430CrossRefGoogle Scholar
  19. 19.
    Geier DA, Audhya T, Kern JK, Geier MR (2010) Blood mercury levels in autism spectrum disorder: is there a threshold level? Acta Neurobiol Exp (Wars) 70(2):177–186Google Scholar
  20. 20.
    Hertz-Picciotto I, Green PG, Delwiche L, Hansen R, Walker C, Pessah IN (2010) Blood mercury concentrations in CHARGE Study children with and without autism. Environ Health Perspect 118(1):161–166PubMedGoogle Scholar
  21. 21.
    Yorbik O, Kurt I, Haşimi A, Oztürk O (2010) Chromium, cadmium, and lead levels in urine of children with autism and typically developing controls. Biol Trace Elem Res 135(1–3):10–15, Epub 2009 Aug 18PubMedCrossRefGoogle Scholar
  22. 22.
    Bradstreet J, Geier DA, Kartzinel JJ, Adams JB, Geier MR (2003) A case–control study of mercury burden in children with autistic spectrum disorders. J Am Phys Surg 8(3):76–79Google Scholar
  23. 23.
    Soden SE, Lowry JA, Garrison CB, Wasserman GS (2007) 24-Hour provoked urine excretion test for heavy metals in children with autism and typically developing controls, a pilot study. Clin Toxicol (Phila) 45(5):476–481, Jun–AugCrossRefGoogle Scholar
  24. 24.
    Nataf R, Skorupka C, Amet L, Lam A, Springbett A (2006) Lathe R Porphyrinuria in childhood autistic disorder: implications for environmental toxicity. Toxicol Appl Pharmacol 214:99–108PubMedCrossRefGoogle Scholar
  25. 25.
    Geier DA, Geier MR (2006) A prospective assessment of porphyrins in autistic disorders: a potential marker for heavy metal exposure. Neurotox Res 10:57–64PubMedCrossRefGoogle Scholar
  26. 26.
    Geier DA, Geier MR (2007) A prospective study of mercury toxicity biomarkers in autistic spectrum disorders. J Toxicology and Environmental Health A70:1723–1730CrossRefGoogle Scholar
  27. 27.
    Geier DA, Kern JK, Garver CR, Adams JB, Audhya T, Nataf R, Geier MR (2009) Biomarkers of environmental toxicity and susceptibility in autism. J Neurol Sci 280:101–108, Epub - 2008 Sep 24PubMedCrossRefGoogle Scholar
  28. 28.
    Woods JS, Armel SE, Fulton DI, Allen J, Wessels K, Simmonds PL, Granpeesheh D, Mumper E, Bradstreet JJ, Echeverria D, Heyer NJ, Rooney JP (2010) Urinary porphyrin excretion in neurotypical and autistic children. Environ Health Perspect 118(10)):1450–1457, Epub 2010 Jun 24PubMedCrossRefGoogle Scholar
  29. 29.
    Windham GC, Zhang L, Gunier R, Croen LA, Grether JK (2006) Autism spectrum disorders in relation to distribution of hazardous air pollutants in the San Francisco bay area. Environ Health Perspect 114(9):1438–1444PubMedCrossRefGoogle Scholar
  30. 30.
    Palmer RF, Blanchard S, Wood R (2009) Proximity to point sources of environmental mercury release as a predictor of autism prevalence. Health Place 15(1):18–24, Epub 2008 Feb 12PubMedCrossRefGoogle Scholar
  31. 31.
    Palmer RF, Blanchard S, Stein Z, Mandell D, Miller C (2006) Environmental mercury release, special education rates, and autism disorder: an ecological study of Texas. Health Place 12(2):203–209PubMedCrossRefGoogle Scholar
  32. 32.
    Shandley K, Austin DW (2011) Ancestry of pink disease (infantile acrodynia) identified as a risk factor for autism spectrum disorders. J Toxicol Environ Health A 74(18):1185–1194PubMedCrossRefGoogle Scholar
  33. 33.
    Adams JB, Baral M, Geis E, Mitchell J, Ingram J, Hensley A, Zappia I, Newmark S, Gehn E, Rubin RA, Mitchell K, Bradstreet J, El-Dahr JM (2009) The severity of autism is associated with toxic metal body burden and red blood cell glutathione levels. J Toxicol 2009(532640):7. doi:10.1155/2009/532640 Google Scholar
  34. 34.
    Adams JB, Baral M, Geis E, Mitchell J, Ingram J, Hensley A, Zappia I, Newmark S, Gehn E, Rubin RA, Mitchell K, Bradstreet J, El-Dahr J (2009) Safety and efficacy of oral DMSA therapy for children with autism spectrum disorders: part A—medical results. BMC Clin Pharmacol 9:16PubMedCrossRefGoogle Scholar
  35. 35.
    Adams JB, Baral M, Geis E, Mitchell J, Ingram J, Hensley A, Zappia I, Newmark S, Gehn E, Rubin RA, Mitchell K, Bradstreet J, El-Dahr J (2009) Safety and efficacy of oral DMSA therapy for children with autism spectrum disorders: part B—behavioral results. BMC Clin Pharmacol 9:17PubMedCrossRefGoogle Scholar
  36. 36.
    Puchyr RF, Bass DA, Gajewski R, Calvin M, Marquardt W, Urek K, Druyan ME, Quig D (1998) Preparation of hair for measurement of elements by inductively coupled plasma-mass spectrometry (ICP-MS). Biol Trace Elem Res 62(3):167–182PubMedCrossRefGoogle Scholar
  37. 37.
    Cohen IL, Schmidt-Lackner S, Romanczyk R, Sudhalter V (2003) The PDD behavior inventory: a rating scale for assessing response to intervention in children with pervasive developmental disorder. J Autism Dev Disord 33(1):31–45PubMedCrossRefGoogle Scholar
  38. 38.
    Rimland B and Edelson S (2000) Autism treatment evaluation checklist: statistical analyses, Autism Research InstituteGoogle Scholar
  39. 39.
    de Magistris L, Familiari V, Pascotto A, Sapone A, Frolli A, Iardino P, Carteni M, De Rosa M, Francavilla R, Riegler G, Militerni R, Bravaccio C (2010) Alterations of the intestinal barrier in patients with autism spectrum disorders and in their first-degree relatives. J Pediatr Gastroenterol Nutr 51(4):418–424PubMedCrossRefGoogle Scholar
  40. 40.
    Rowland IR, Grasso P, Davies MJ (1975) The methylation of mercuric chloride by human intestinal bacteria. Experientia 31:1064–1065PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2012

Authors and Affiliations

  • James B. Adams
    • 1
  • Tapan Audhya
    • 2
  • Sharon McDonough-Means
    • 3
  • Robert A. Rubin
    • 4
  • David Quig
    • 5
  • Elizabeth Geis
    • 1
  • Eva Gehn
    • 1
  • Melissa Loresto
    • 1
  • Jessica Mitchell
    • 6
  • Sharon Atwood
    • 1
  • Suzanne Barnhouse
    • 1
  • Wondra Lee
    • 1
  1. 1.Arizona State UniversityTempeUSA
  2. 2.Health DiagnosticsSouth AmboyUSA
  3. 3.Integrative Developmental PediatricsTucsonUSA
  4. 4.Department of MathematicsWhittier CollegeWhittierUSA
  5. 5.Doctor’s DataSt. CharlesUSA
  6. 6.Southwest College of Naturopathic MedicineTempeUSA

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