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Effects of Selenium Supplementation on Expression of Endothelin-1 and Its Receptors in Pulmonary Microvascular Endothelial Cells from Chick Embryos

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Abstract

The objective of this study was to evaluate the effects of supplemental selenium (Se) on expression of endothelin-1 (ET-1) and its receptors in cultured chick embryos pulmonary microvascular endothelial cells (PMVECs). To accomplish this, PMVECs were treated in Se-deficient or Se-supplement (12, 24, 50, 100 ng/ml) culture medium for 48 h. Low Se medium was achieved by reducing serum concentrations and the essential growth factors were added. After the incubation, the effects of supplemental Se on ET-1 and its receptors gene expression were assessed by quantitative real-time PCR (qRT-PCR). Compared with the control group, our results showed that among the different concentrations of Se supplement, the levels of ET-1 gene expression treated with both the moderate Se doses (24, 50 ng/ml, P < 0.01, P < 0.01, respectively) and the high doses (100 ng/ml, P < 0.05) were noticeably decreased, the low-dose group (12 ng/ml), which showed no changes. Meanwhile, Se supplement (24, 50, 100 ng/ml) was found to be effective in reducing the expression levels of ETA (P < 0.01, P < 0.05, P < 0.05, respectively) in cultured PMVECs grown in low Se medium. However, there were no significant changes (P > 0.05) in ETB mRNA levels during the cell proliferation. These observations indicated that Se may play both direct and indirect role in the regulation of ET-1 and its receptors gene expression and their production in avian PMVECs. Se supplement decreases in ET-1 and ETA production in Se-deficient PMVECs may partly explain the mechanism of the protective effects of the Se on the cardiovascular system.

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References

  1. Epstein HF (1995) Endothelins. New Engl J Med 333:356–363

    Article  Google Scholar 

  2. Lerman A, Brooks SE, Hallett JW, Heublein DM, Sanberg SM, Burnett JC (1991) Circulating and tissue endothelin immunoreactivity in advanced atherosclerosis. New Engl J Med 325:997–1001

    Article  PubMed  CAS  Google Scholar 

  3. Miyauchi T, Yanagisawa M, Tomizawa T, Sugishita Y, Suzuki N, Fujino M, Ajisaka R, Goto K, Masaki T (1989) Increased plasma concentration of endothelin-1 and big endothelin-1 in acute myocardial infarction. Lancet 2:53–54

    Article  PubMed  CAS  Google Scholar 

  4. Yanagisawa M, Kurihara H, Kimura S, Tomobe Y, Kobayashi M, Mitsui Y, Yazaki Y, Goto K, Masaki T (1988) A novel potent vasoconstrictor peptide produced by vascular endothelial cells. Nature 332:411–415

    Article  PubMed  CAS  Google Scholar 

  5. Hirata NY, Takagi Y, Fukuda Y, Marumo F (1989) Endothelin is a potent mitogen for rat vascular smooth muscle cell. Atherosclerosis 78:225–228

    Article  PubMed  CAS  Google Scholar 

  6. Takuwa N, Takuwa Y, Yanagisawa M, Yamashita K, Masaki T (1989) A novel vasoactive peptide endothelin stimulates mitogenesis through inositol lipid turnover in Swiss 3 T3 fibroblasts. J Biol Chem 264:7856–7861

    PubMed  CAS  Google Scholar 

  7. Zeballos GA, An SJ, Wu JM (1991) Endothelin-1 secretion by human fibroblasts in culture: effect of cell density and IFN-beta. Biochem Int 25:845–852

    PubMed  CAS  Google Scholar 

  8. Gomez AP, Moreno MJ, Iglesias A, Coral PX, Hernandez A (2007) Endothelin 1, its endothelin type A receptor, connective tissue growth factor, platelet-derived growth factor, and adrenomedullin expression in lungs of pulmonary hypertensive and nonhypertensive chickens. Poult Sci 86:909–916

    PubMed  CAS  Google Scholar 

  9. Stanford SJ, Walters MJ, Mitchell JA (2004) Carbon monoxide inhibits endothelin-1 release by human pulmonaryartery smooth muscle cells. Eur J Pharmacol 486:349–352

    Article  PubMed  CAS  Google Scholar 

  10. Cacoub P, Dorent R, Nataf P, Carayon A, Riquet M, Noe E, Piette JC, Godeau P, Gandjbakhch I (1997) Endothelin-1 in the lungs of patients with pulmonary hypertension. Cardiovasc Res 33:196–200

    Article  PubMed  CAS  Google Scholar 

  11. Giaid A (1998) Nitric oxide and endothelin-1 in pulmonary hypertension. Chest 114:208S–212S

    Article  PubMed  CAS  Google Scholar 

  12. Chen YF, Oparil S (2000) Endothelin and pulmonary hypertension. J Cardiovasc Pharm 35:S49–S53

    Article  CAS  Google Scholar 

  13. Mazurek JA, Salamon J, Zolty R (2012) Endothelin-1 levels in pulmonary hypertension: a comparison between pulmonary arterial hypertension and diastolic heart failure-induced pulmonary hypertension. J Am Coll Cardiol 59:E1617

    Article  Google Scholar 

  14. Shao D, Park JE, Wort SJ (2011) The role of endothelin-1 in the pathogenesis of pulmonary arterial hypertension. Pharmacol Res 63:504–511

    Article  PubMed  CAS  Google Scholar 

  15. Takahashi H, Soma S, Muramatsu M, Oka M, Fukuchi Y (2001) Upregulation of ET-1 and its receptors and remodeling in small pulmonary veins under hypoxic conditions. Am J Physiol Lung Cell Mol Physiol 280:L1104–L1114

    PubMed  CAS  Google Scholar 

  16. Thaete LG, Jilling T, Synowiec S, Khan S, Neerhof MG (2007) Expression of endothelin 1 and its receptors in the hypoxic pregnant rat. Biol Reprod 77:526–532

    Article  PubMed  CAS  Google Scholar 

  17. Yang Y, Gao MY, Wu ZL, Guo Y (2010) Genistein attenuates low temperature induced pulmonary hypertension in broiler chicks by modulating endothelial function. Eur J Pharmacol 649:242–248

    Article  PubMed  CAS  Google Scholar 

  18. Zhou DH, Wu J, Yang SJ, Cheng DC, Guo DZ (2008) Intravenous endothelin-1 triggers pulmonary hypertension syndrome (ascites) in broilers. Vet Med-Czech 53:381–391

    CAS  Google Scholar 

  19. Aftab U, Khan AA (2005) Strategies to alleviate the incidence of ascites in broilers: a review. Braz J Poultry Sci 7:199–204

    Google Scholar 

  20. Wang Y, Fu L (2011) Forms of selenium affect its transport, uptake and glutathione peroxidase activity in the Caco-2 cell model. Biol Trace Elem Res. doi:10.1007/s12011-012-9395-8

  21. Brigelius-Flohe R, Banning A, Schnurr K (2003) Selenium-dependent enzymes in endothelial cell function. Antioxid Redox Sign 5:205–215

    Article  CAS  Google Scholar 

  22. Li JX, Yang ZQ, Wang XZ, Zhang SZ, Feng X, Qi ZM, Meng JR, Sun WH (2003) Pathogenesis implicating with oxidative stress and preventions of ascites syndrome in broiler. Chin J Vet Sci 23:372–374

    CAS  Google Scholar 

  23. Hara S, Shoji Y, Sakurai A, Yuasa K, Himeno S, Imura N (2001) Effects of selenium deficiency on expression of selenoproteins in bovine arterial endothelial cells. Biol Pharm Bull 24:754–759

    Article  PubMed  CAS  Google Scholar 

  24. Jahnova E, Horvathova M, Gazdik F, Weissova S (2002) Effects of selenium supplementation on expression of adhesion molecules in corticoid-dependent asthmatics. Bratisl Lek Listy 103:12–16

    PubMed  CAS  Google Scholar 

  25. Gomez AP, Moreno MJ, Baldrich RM, Hernandez A (2008) Endothelin-1 messenger [corrected] ribonucleic acid expression in pulmonary hypertensive and nonhypertensive chickens. Poult Sci 87:1395–1401

    Article  PubMed  CAS  Google Scholar 

  26. Sordillo LM, SooHoo H, Aherne KM, Reddy CC, Hogan JS (1998) A method to reduce glutathione peroxidase levels in primary endothelial cell cultures. Meth Cell Sci 19:243–253

    Article  Google Scholar 

  27. Li YC, Jiang W, Zhou M, Xue Y, Li YC (2010) Improvement of method for primary cultivation and identification of rat pulmonary microvascular endothelial cells. J Shanghai Jiaotong Univ (Med Sci) 30:1440–1443

    Google Scholar 

  28. Meng G, Zhao J, Lv XH, Ding RG (2004) Improvement of rat pulmonary microvascular endothelial cell primary culture methods. Bull Acad Mil Med Sci 33:567–569

    Google Scholar 

  29. Talavera-Adame D, Ng TT, Gupta A, Kurtovic S, Wu GD, Dafoe DC (2011) Characterization of microvascular endothelial cells isolated from the dermis of adult mouse tails. Microvasc Res 82:97–104

    Article  PubMed  CAS  Google Scholar 

  30. Li AY, Zhao LH, Liu YJ, Wang YQ, Zhou FJ (1997) Effects of selenium on the plasma endothelin in rabbits with atherosclerosis. Stud Trace Elem Health 14:5–7

    Google Scholar 

  31. Liu HM, Huang KX, Yang JS, Chen ZX, Xu HB (2002) Effects of cholesterol oxides on plasma prostacyclin and endothelin levels of rats in different selenium status. Acta Nutrimenta Sin 24:70–74

    CAS  Google Scholar 

  32. Ozkan S, Malayoğlu HB, Yalçin S, Karadas F, Koçtürk S, Cabuk M, Oktay G, Ozdemir S, Ozdemir E, Ergül M (2007) Dietary vitamin E (alpha-tocopherol acetate) and selenium supplementation from different sources: performance, ascites-related variables and antioxidant status in broilers reared at low and optimum temperatures. Br Poult Sci 48:580–593

    Article  PubMed  CAS  Google Scholar 

  33. Hassanpour H, Momtaz H, Shahgholian L, Bagheri R, Sarfarzaz S, Heydaripoor B (2010) Gene expression of endothelin-1 and its receptors in the heart of broiler chickens with T3-induced pulmonary hypertension. Res Vet Sci. doi:10.1016/j.rvsc.2010.09.019

  34. Bauer M, Wilkens H, Langer F, Schneider SO, Lausberg H, Schäfers HJ (2002) Selective upregulation of endothelin B receptor gene expression in severe pulmonary hypertension. Circulation 105:1034–1036

    Article  PubMed  CAS  Google Scholar 

  35. Hamal KR, Wideman RF, Anthony NB, Erf GF (2010) Differential expression of vasoactive mediators in microparticle-challenged lungs of chickens that differ in susceptibility to pulmonary arterial hypertension. Am J Physiol Regul Integr Comp Physiol 298:R235–R242

    Article  PubMed  CAS  Google Scholar 

  36. Cao YZ, Reddy CC, Sordillo LM (2000) Altered eicosanoid biosynthesis in selenium-deficient endothelial cells. Free Radic Biol Med 28:381–389

    Article  PubMed  CAS  Google Scholar 

  37. Sirmali M, Uz E, Sirmali R, Kilbaş A, Yilmaz HR, Altuntaş I, Naziroğlu M, Delibaş N, Vural H (2007) Protective effects of erdosteine and vitamins C and E combination on schemia–reperfusion-induced lung oxidative stress and plasma copper and zinc levels in a rat hind limb model. Biol Trace Elem Res 118:43–52

    Article  PubMed  CAS  Google Scholar 

  38. Nazıroglu M (2009) Role of selenium on calcium signaling and oxidative stress-induced molecular pathways in epilepsy. Neurochem Res 34:2181–2191

    Article  PubMed  Google Scholar 

Download references

Acknowledgment

This study was supported by the National Natural Science Foundation of China (no. 30972222) and the Tibet Autonomous Region Science Fund.

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Authors and Affiliations

  1. College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, People’s Republic of China

    Jianfeng Gao, Ding Zhang, Kerong Zhang, Mengyuan Liu, Zhaoqing Han & Jiakui Li

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  1. Jianfeng Gao
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  2. Ding Zhang
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  3. Kerong Zhang
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  4. Mengyuan Liu
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  5. Zhaoqing Han
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  6. Jiakui Li
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Correspondence to Jiakui Li.

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Jianfeng Gao and Ding Zhang contributed equally to this work.

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Gao, J., Zhang, D., Zhang, K. et al. Effects of Selenium Supplementation on Expression of Endothelin-1 and Its Receptors in Pulmonary Microvascular Endothelial Cells from Chick Embryos. Biol Trace Elem Res 150, 173–177 (2012). https://doi.org/10.1007/s12011-012-9521-7

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  • DOI: https://doi.org/10.1007/s12011-012-9521-7

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