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Catalase and Lipid Peroxidation Values in Serum of Tunisian Patients with Pemphigus Vulgaris and Foliaceus

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Abstract

Pemphigus is an autoimmune disorder resulting from the interaction between autoantibodies and desmoglein. Oxidative stress seems to be responsible for the onset/aggravation of many human diseases. Actually, it is considered as one of the several factors for the etiopathogenesis of pemphigus. The present study aims to evaluate the oxidative state in the sera of pemphigus vulgaris and pemphigus foliaceus patients by assessing lipid peroxidation, proteins oxidation, and antioxidant enzyme activity. This study included 36 pemphigus vulgaris and 42 pemphigus foliaceus patients as well as a group of controls consisting of 78 healthy volunteers. Malondialdehyde levels (p < 0.001) and catalase activity (p < 0.001) are higher in both groups of patients than in the control group. The two groups of patients showed a nonsignificant decrease in the thiol groups compared with the healthy one. A nonsignificant difference was shown between pemphigus vulgaris and pemphigus foliaceus patients, except for the catalase which shows an increase in the pemphigus vulgaris group. We have also found significant correlations between serum oxidative stress marker levels and serum anti-desmoglein antibody levels in the two pemphigus groups. These findings underline the implication of oxidative stress in the physiopathology of pemphigus by the increase in the autoantibodies’ reactivity.

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Abbreviations

TBA:

Thiobarbituric acid reactivity

MDA:

Malondialdehyde

CAT:

Catalase

SH:

Thiol

ROS:

Reactive oxygen species

DNPH:

2,4-dinitrophenylhydrazine

TBARs:

Thiobarbituric acid-reactive species assay

OD:

Optical density

NO:

Nitric oxide

DTPA:

Tris–cacodylic acid–diethylenetriaminepenta-acetic acid

H2O2 :

Hydrogen peroxide

PF:

Pemphigus foliaceus

PV:

Pemphigus vulgaris

RBC:

Red blood cells

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Acknowledgments

We thank Ms. Nadia Ben Hamed, proficient in the English language for proofreading the article. This work is dedicated to the memory of Dr. Hentati Basma, the last director of the Higher Institute of Biotechnology of Sfax, as well as the Laboratory of Biotechnology and Pathology. We will never forget her and she is always in our heart. This work was supported by grants from Direction Générale de la Recherche Scientifique et Technique (Tunisie).

Conflict of interest

None declared

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Authors and Affiliations

  1. Unité de recherche d’״auto-immunité et immunogénétique״, hôpital Habib Bourguiba, Sfax, Tunisia

    Olfa Abida, Mourad Ben Ayed & Hatem Masmoudi

  2. Laboratoire d’analyse, valorisation et sécurité des aliments, groupe de recherche Biotechnologie et pathologies, Institut Supérieur de Biotechnologie de Sfax, Université de Sfax, Sfax, Tunisia

    Riadh Ben Mansour, Bochra Gargouri & Saloua Lassoued

  3. Service de Dermatologie, hôpital Hédi Chaker, Sfax, Tunisia

    Abderrahmen Masmoudi & Hamida Turki

  4. Immunology Department, Habib Bourguiba Hospital, PTT El Bustan, BP 10 A, 3099, Sfax, Tunisia

    Olfa Abida

Authors
  1. Olfa Abida
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  2. Riadh Ben Mansour
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  3. Bochra Gargouri
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  4. Mourad Ben Ayed
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  5. Abderrahmen Masmoudi
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  6. Hamida Turki
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  7. Hatem Masmoudi
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  8. Saloua Lassoued
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Correspondence to Olfa Abida.

Additional information

Olfa Abida and Riadh Ben Mansour equally contributed to this work.

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Abida, O., Ben Mansour, R., Gargouri, B. et al. Catalase and Lipid Peroxidation Values in Serum of Tunisian Patients with Pemphigus Vulgaris and Foliaceus. Biol Trace Elem Res 150, 74–80 (2012). https://doi.org/10.1007/s12011-012-9497-3

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  • DOI: https://doi.org/10.1007/s12011-012-9497-3

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