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Zinc Supplementation Attenuates High Glucose-Induced Epithelial-to-Mesenchymal Transition of Peritoneal Mesothelial Cells

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Abstract

Zinc (Zn) plays an important role in preventing many types of epithelial-to-mesenchymal transition (EMT)-driven fibrosis in vivo. But its function in the EMT of the peritoneal mesothelial cells (PMCs) remains unknown. Here, we studied the Zn effect on the high glucose (HG)-induced EMT in the rat PMCs (RPMCs) and the underlying molecular mechanisms. We found that Zn supplementation significantly inhibited TGF-β1 and ROS production, and attenuated the HG-induced EMT in the RPMCs, likely through inhibition of MAPK, NF-κB, and TGF-β/Smad pathways.

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Abbreviations

RPMCs:

Rat peritoneal mesothelial cells

HG:

High glucose

CAPD:

Continuous ambulatory peritoneal dialysis

EMT:

Epithelial-to-mesenchymal transition

MAPK:

Mitogen-activated protein kinase

JNK:

Jun N-terminal kinase

MTT:

3-[4,5-Dimethylthiazol-2-y]-2,5-diphenyltetrazolium bromide

DMEM:

Dulbecco’s modified Eagle medium

DMSO:

Dimethyl sulfoxide

SMA:

Smooth muscle cell actin

FITC:

Fluorescein isothiocyanate

TPEN:

N,N,N′,N′-Tetrakis(2-pyridylmethyl) ethylenediamine

RIPA:

Radioimmune precipitation assay

TGF:

Transforming growth factor

PBS:

Phosphate-buffered saline

DCF-DA:

2,7-Dichlorofluorescein diacetate

TBS:

Tris-buffered saline

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Authors and Affiliations

  1. Department of Nephrology, the First Affiliated Hospital, China Medical University, 155th Nanjing North Street, Shenyang, Liaoning, 110001, People’s Republic of China

    Xiuli Zhang, Jun Wang, Yi Fan, Lina Yang, Lining Wang & Jianfei Ma

  2. Department of Nephrology, Benxi Railway Hospital, Benxi, Liaoning, People’s Republic of China

    Xiuli Zhang

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  1. Xiuli Zhang
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  2. Jun Wang
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Correspondence to Jianfei Ma.

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Zhang, X., Wang, J., Fan, Y. et al. Zinc Supplementation Attenuates High Glucose-Induced Epithelial-to-Mesenchymal Transition of Peritoneal Mesothelial Cells. Biol Trace Elem Res 150, 229–235 (2012). https://doi.org/10.1007/s12011-012-9451-4

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  • DOI: https://doi.org/10.1007/s12011-012-9451-4

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