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An Organoselenium Drug with Antioxidant Activity and Free Radical Scavenging Capacity In Vitro

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Abstract

Organoselenum compounds have been reported to have a wide range of pharmacological properties. Amine-based diselenide, (Z)-N-(4-methylbenzylidene)-1-(2-((2-(1-((E)-4-methyl benzylideneamino)ethyl)phenyl)diselanyl)phenyl)ethanamine ethyl)phenyl) diselanyl) phenyl) ethylimino) methyl)phenol (compound A), and diphenyl diselenide (PhSe)2 were screened for in vitro antioxidant activity. Compound A and (PhSe)2 were tested against sodium nitroprusside (SNP)- and Fe(II)-induced thiobarbituric acid-reactive species (TBARS) in rat brain homogenates. The radical scavenging activity was measured by 1,1-diphenyl-2-picrylhydrazyl assay. Both compounds A and (PhSe)2 decreased Fe(II)- and SNP-stimulated TBARS production in rat brain homogenates. Compound A exhibited the strongest antioxidant activity in the radical scavenging assay, although (PhSe)2, the simplest of the diaryl diselenide, presented no activity. In conclusion, the results of the present investigation indicated that compound A and (PhSe)2 had preventive effects against SNP- and Fe(II)-induced oxidative stress in rat brain homogenates. The amine group in the organic moiety dramatically changed the potency of amine-based diselenide.

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Acknowledgments

Mohammad Ibrahim is a beneficiary of the TWAS-CNPq doctoral fellowship program. The financial support of TWAS, CNPq, is acknowledged.

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Authors and Affiliations

  1. Programa de Pós-Graduação em Ciências Biológicas—Bioquímica Toxicológica, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Santa Maria, CEP 97105-900, Rio Grande do Sul, Brazil

    Mohammad Ibrahim, Waseem Hassan & Joao B. T. Rocha

  2. Department of Chemistry, Abdul Wali Khan University Mardan (AWKUM), Mardan, Khyber Pakhtunkhwa, Pakistan

    Mohammad Ibrahim

  3. Institute of Chemical Sciences, University of Peshawar, Peshawar, Khyber Pakhtunkhwa, Pakistan

    Waseem Hassan

  4. Departamento de Química, Universidade Federal de São Carlos, São Carlos, SP, Brazil

    Anna Maria Deobald

  5. Laboratório de Bioenergética e Estresse Oxidativo, Departamento de Bioquímica, Centro de Ciências Biológicas, Universidade Federal de Santa Catarina, Florianópolis, Santa Catarina, Brazil

    Antonio Luis Braga

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  1. Mohammad Ibrahim
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  2. Waseem Hassan
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  3. Anna Maria Deobald
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  4. Antonio Luis Braga
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  5. Joao B. T. Rocha
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Correspondence to Mohammad Ibrahim.

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Ibrahim, M., Hassan, W., Deobald, A.M. et al. An Organoselenium Drug with Antioxidant Activity and Free Radical Scavenging Capacity In Vitro. Biol Trace Elem Res 149, 399–404 (2012). https://doi.org/10.1007/s12011-012-9440-7

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  • DOI: https://doi.org/10.1007/s12011-012-9440-7

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