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Effect of Different Levels of Copper and Molybdenum Supplements on Serum Lipid Profiles and Antioxidant Status in Cashmere Goats

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Abstract

An experiment was conducted to investigate on the effects of different levels of copper (Cu: 0, 19, and 38 mg/kg) and molybdenum (Mo: 0 and 5 mg/kg) supplements and the interaction of these two factors on serum lipid profiles and antioxidant status in cashmere goats during the cashmere fiber growing period. Thirty-six Liaoning cashmere goats (approximately 1.5 years of age; 27.53 ± 1.38 kg of body weight) were assigned to one of six treatments in a completely randomized design involving a 2 × 3 factorial arrangement. Goats were housed in individual pens and fed with Chinese wild rye- and alfalfa hay-based diet containing 4.72 mg Cu/kg, 0.16 mg Mo/kg, and 0.21 % S for 84 days. Blood samples were collected on day 84. The triglyceride concentration did not differ among treatments (P > 0.05). Supplemental Cu, regardless of Mo level, decreased (P < 0.05) the concentrations of serum total cholesterol and low density lipoprotein cholesterol, and increased (P < 0.05) the concentration of serum high density lipoprotein cholesterol, but there were no differences (P > 0.05) in these values between Cu-supplemented groups. Supplemental Cu increased (P < 0.05) the activities of serum ceruloplasmin (Cp), Cu–zinc superoxide dismutase (SOD), and glutathione peroxidase (GSH-Px), and decreased (P < 0.05) the malondialdehyde content. The serum GSH-Px activity was also increased (P < 0.05) by Mo supplementation. There was a tendency of the interaction effects of Cu and Mo on the activities of Cp (P = 0.094), SOD (P = 0.057), and GSH-Px (P = 0.062), and goats fed with 19 mg Cu/kg in the absence of Mo tended to show the highest serum SOD activity, while goats fed with 38 mg Cu/kg with 5 mg Mo/kg tended to show the highest values of serum Cp and GSH-Px. Addition of Cu, Mo, or their interaction had no influence (P > 0.05) on the activities of serum glutamate oxaloacetate transaminase, glutamate pyruvate transaminase, and lactate dehydrogenase, and the concentrations of serum glucose and total protein. In conclusion, addition of 19 mg Cu/kg in the absence of Mo (the total dietary Cu level of 23.72 mg/kg) was recommended for altering the fat metabolism and obtaining the optimal antioxidant activity of cashmere goats, while 38 mg Cu/kg should be supplemented when 5 mg Mo/kg was added in the basal diet (the total dietary level of 42.72 mg Cu/kg, 5.16 mg Mo/kg, and 0.21 % S) during the cashmere growing period.

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References

  1. Davis KG, Mertz W (1987) Copper. In: Mertz W (ed) Trace elements in human and animal nutrition. Academic Press, NY

    Google Scholar 

  2. Jenkins KJ, Kramer JKG (1989) Influence of dietary copper on lipid composition in calf tissues. J Dairy Sci 72:2582–2591

    Article  PubMed  CAS  Google Scholar 

  3. Solaiman SG, Shoemaker CE, Jones WR, Kerth CR (2006) The effects of high levels of supplemental copper on the serum lipid profiles, carcass traits, and carcass composition in goat kids. J Anim Sci 84:171–177

    PubMed  CAS  Google Scholar 

  4. Cummins KA, Solaiman SG, Bergen WG (2008) The effect of dietary copper supplementation on fatty acid profile and oxidative stability of adipose depots in Boer x Spanish goats. J Anim Sci 86:390–396

    Article  PubMed  CAS  Google Scholar 

  5. Engle TE, Spears JW, Wright CL, Armstrong TA (1999) Dietary copper affects lipid and cholesterol metabolism in finishing steers. J Anim Sci 77(Suppl 1):253–254

    Google Scholar 

  6. Engle TE, Spears JW, Armstrong TA, Wrigh CL, Odle J (2000) Effects of dietary copper source and concentration on carcass characteristics and lipid on cholesterol metabolism in growing and finishing steers. J Anim Sci 78:1053–1059

    PubMed  CAS  Google Scholar 

  7. Engle TE, Spears JW, Xi L, Edens FW (2000) Dietary copper affects on lipid metabolism and circulating catecholamine concentrations in finishing steers. J Anim Sci 78:2737–2744

    PubMed  CAS  Google Scholar 

  8. Engle TE, Spears JW (2000) Dietary copper affects on lipid metabolism, performance and ruminal fermentation in finishing steers. J Anim Sci 78:2452–2458

    PubMed  CAS  Google Scholar 

  9. Sinnett-Smith PA, Woolliams JA (1987) Adipose tissue metabolism and cell size: variation between subcutaneous sites and the effect of copper supplementation. Anim Prod 45:75–80

    Article  CAS  Google Scholar 

  10. Cheng JB, Fan CY, Zhu XP, Zhang W, Yan XG, Wang RL, Jia ZH (2008) Effects of dietary copper source and level on performance, carcass characteristics and lipid metabolism in lambs. Asian Australas J Anim Sci 21(5):685–691

    CAS  Google Scholar 

  11. Datta C, Mondal MK, Biswas P (2007) Influence of dietary inorganic and organic form of copper salt on performance, plasma lipids and nutrient utilization of Black Bengal (Capra hircus) goat kids. Anim Feed Sci Tech 135:191–209

    Article  CAS  Google Scholar 

  12. Mondala MK, Biswas P, Roya B, Mazumdarb D (2007) Effect of copper sources and levels on serum lipid profiles in Black Bengal (Capra hircus) kids. Small Rumin Res 67:28–35

    Article  Google Scholar 

  13. Lee J, Knowles SO, Judson GJ (2002) Trace element and vitamin nutrition of grazing sheep. In: Sheep nutrition. CABI Publishing, Wallingford, UK

  14. Saleha MA, Al-Salahyb MB, Sanousic SA (2008) Corpuscular oxidative stress in desert sheep naturally deficient in copper. Small Rumin Res 80:33–38

    Article  Google Scholar 

  15. Blood DC, Radostits OM, Arundel JH, Gay CC (1992) Veterinary medicine. A textbook of the diseases of cattle, sheep, pigs, goats and horses, 7th edn. Bailliere Tindall, London

    Google Scholar 

  16. Gooneratne SR, Buchley WT, Christensen DA (1989) Review of copper deficiency and metabolism in ruminants. Can J Anim Sci 69:819–845

    Article  Google Scholar 

  17. Humphries WR, Phillippo M, Yong BW, Bremner I (1983) The influence of dietary iron and molybdenum on copper metabolism in calves. Br J Nutr 49:77–86

    Article  PubMed  CAS  Google Scholar 

  18. Suttle NF (1991) The interactions between copper, molybdenum and sulfur in ruminant nutrition. Annu Rev Nutr 11:121–140

    Article  PubMed  CAS  Google Scholar 

  19. Hayes C (1995) Some nutritional factors affecting the performance of early born lambs. Thesis for M Agr Sc, University College Dublin, National University of Ireland, Dublin

  20. Pott EB, Henry PR, Zanetti MA, Rao PV, Hinderberger EJ Jr, Ammerman CB (1999) Effects of high dietary molybdenum concentration and duration of feeding time on molybdenum and copper metabolism in sheep. Anim Feed Sci Tech 79:93–105

    Article  CAS  Google Scholar 

  21. Pott EB, Henry PR, Rao PV, Hinderberger EG Jr, Ammerman CB (1999) Estimated relative bioavailability of supplemental inorganic molybdenum sources and their effect on tissue molybdenum and copper concentrations in lambs. Anim Feed Sci Tech 79:107–117

    Article  CAS  Google Scholar 

  22. Crosby TF, Quinn PJ, Callan JJ, O'Hara M (2004) Effects of floor type and dietary molybdenum content on the liver copper concentration at slaughter and performance of intensively finished lambs. Livestock Sci 90:181–190

    Article  Google Scholar 

  23. Galbraith H, Chigwada W, Scaife JR, Humphries WR (1997) The effect of dietary molybdenum supplementation on tissue copper concentrations, mohair fiber and carcass characteristics of growing Angora goats. Anim Feed Sci Tech 67:83–90

    Article  CAS  Google Scholar 

  24. Zhang W, Wang RL, Kleemann DO, Lu DX, Zhu XP, Zhang CX, Jia ZH (2008) Effects of dietary copper on nutrient digestibility, growth performance and plasma copper status in cashmere goats. Small Rumin Res 74:188–193

    Article  Google Scholar 

  25. Zhang W, Wang RL, Zhu XP, Kleemann DO, Yue CW, Jia ZH (2007) Effects of dietary copper on ruminal fermentation, nutrient digestibility and fiber characteristics in cashmere goats. Asian Australas J Anim Sci 20:1843–1848

    CAS  Google Scholar 

  26. Zhang W, Wang RL, Kleemann DO, Guo MY, Xu JH, Zhang CX, Jia ZH (2009) Effects of dietary copper on growth performance, nutrient digestibility and fiber characteristics in cashmere goats during the cashmere slow-growing period. Small Rumin Res 85:58–62

    Article  Google Scholar 

  27. Wittenberg KM, Boila RJ (1988) Supplementary copper for growing cattle consuming diets high in molybdenum or molybdenum plus sulfur. Can J Anim Sci 68:1143–1154

    Article  CAS  Google Scholar 

  28. Gengelbach GP, Ward JD, Spears JW (1994) Effect of dietary copper, iron and molybdenum on growth and copper status of beef cows and calves. J Anim Sci 72:2722–2727

    PubMed  CAS  Google Scholar 

  29. Ward JD, Spears JW (1997) Long-term effects of consumption of low-copper diets with or without supplemental molybdenum on copper status, performance, and carcass characteristics of cattle. J Anim Sci 75:3057–3065

    PubMed  CAS  Google Scholar 

  30. NRC (1981) Nutrient requirements of goats. National Academy Press, Washington

    Google Scholar 

  31. AOAC (1990) Official methods of analysis, 15th edn. Association of Official Analytical Chemists, Washington

    Google Scholar 

  32. Van Soest PJ, Robertson JB, Lewis BA (1991) Methods of dietary fiber, neutral detergent fiber and non-starch polysaccharides in relation to animal nutrition. J Dairy Sci 74:3583–3597

    Article  PubMed  Google Scholar 

  33. SAS (1988) Statistical analysis system. SAS user's guide: statistics, SAS Inst Inc, Cary

  34. Kim S, Chao PY, Allen GD (1992) Inhibition of elevated hepatic glutathione abolishes copper deficiency cholesterolemia. FASEB J 6:2467–2471

    PubMed  CAS  Google Scholar 

  35. Lefevre M, Keen CL, Lonnerdal B, Hurley LS, Schneeman BO (1986) Copper deficiency induced hypocholesterolemia: effects on HDL subfractions and hepatic lipoprotein activity in the rat. J Nutr 116:1735–1746

    PubMed  CAS  Google Scholar 

  36. Uriu-Adams JY, Keen CL (2005) Copper, oxidative stress, and human health. Mol Aspects Med 26:268–298

    Article  PubMed  CAS  Google Scholar 

  37. Xin Z, Waterman DF, Hemken RW, Harmon RJ (1991) Effects of copper status on neutrophil function, superoxide dismutase, and copper distribution in steers. J Dairy Sci 74:3078–3085

    Article  PubMed  CAS  Google Scholar 

  38. Senthilkumar P, Nagalakshmi D, Ramana Reddy Y, Sudhakar K (2009) Effect of different level and source of copper supplementation on immune response and copper dependent enzyme activity in lambs. Trop Anim Health Prod 41:645–653

    Article  PubMed  CAS  Google Scholar 

  39. Solaiman SG, Maloney MA, Qureshi MA, Davis GD, Andrea G (2001) Effects of high copper supplements on performance, health, plasma copper and enzymes in goats. Small Rumin Res 41:127–139

    Article  PubMed  Google Scholar 

  40. Solaiman SG, Shoemaker CE, D'Andrea GH (2006) The effect of high dietary Cu on health, growth performance, and Cu status in young goats. Small Rumin Res 66:85–91

    Article  Google Scholar 

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Acknowledgments

The research was supported by the Key Program of the National Natural Science Foundation of China (project no. 30901032; Beijing, People's Republic of China). This was also supported in part by a grant from the National Key Technologies R & D Program (project no. 2009BADA5B04; Beijing, People's Republic of China).

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Authors and Affiliations

  1. State Key Laboratory for Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing, 100193, People’s Republic of China

    Wei Zhang, Yu Zhang, Zhi H. Jia & Run L. Wang

  2. Liaoning Cashmere Goat Breeding Center, Liaoning, 111001, People’s Republic of China

    Shi W. Zhang & Xian Z. Song

  3. Department of Animal Science, Guangdong Ocean University, Zhanjiang, 524088, People’s Republic of China

    Run L. Wang

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  1. Wei Zhang
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  2. Yu Zhang
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  3. Shi W. Zhang
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  5. Zhi H. Jia
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Correspondence to Run L. Wang.

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Zhang, W., Zhang, Y., Zhang, S.W. et al. Effect of Different Levels of Copper and Molybdenum Supplements on Serum Lipid Profiles and Antioxidant Status in Cashmere Goats. Biol Trace Elem Res 148, 309–315 (2012). https://doi.org/10.1007/s12011-012-9380-2

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