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Effect of Copper on the Expression of IGF-1 from Chondrocytes in Newborn Piglets In Vitro

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Abstract

The experiment was performed to evaluate the influence of copper supplementation on insulin-like growth factor-1 (IGF-1) mRNA expression in chondrocytes of newborn pigs. Chondrocytes were isolated and cultured in media containing 15% fetal calf serum supplemented with 0, 15.6, 31.2, and 62.5 μmol/L copper in 90-mm culture plate. After 0, 12, 24, and 48 h, total RNA was isolated from chondrocytes. Then, IGF-1 mRNA expression was determined by semiquantitative RT-PCR. The results showed that the expression of IGF-1 mRNA, adjusted for β-actin expression, was increased in the culture media added to 15.6, 31.2, and 62.5 μmol/L copper, respectively. In the present experiment, the optimal copper concentration and optimal culture time for the expression of IGF-1 mRNA were 31.2 μmol/L and 48 h, respectively.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (grant nos. 31072178, 30600441, 30972212, and 30871897) and by the Science Fund for Distinguished Young Scholars of Jilin University (grant no. 201100009).

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Authors and Affiliations

  1. College of Animal Science and Veterinary Medicine, Jilin University, Changchun, 130062, China

    Jianguo Wang, Xiaoyan Zhu, Guanghong Xie, Yonghong Zhang, Zhaoxi Liu, Liang Zhang, Chenxu Zhao, Wentao Yang, Xiaoxia Shi, Zhe Wang & Guowen Liu

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  1. Jianguo Wang
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  2. Xiaoyan Zhu
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  3. Guanghong Xie
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  4. Yonghong Zhang
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  5. Zhaoxi Liu
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  6. Liang Zhang
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  7. Chenxu Zhao
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  8. Wentao Yang
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  9. Xiaoxia Shi
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  10. Zhe Wang
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  11. Guowen Liu
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Corresponding authors

Correspondence to Zhe Wang or Guowen Liu.

Additional information

Jianguo Wang, Xiaoyan Zhu, and Guanghong Xie contributed equally to this study.

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Wang, J., Zhu, X., Xie, G. et al. Effect of Copper on the Expression of IGF-1 from Chondrocytes in Newborn Piglets In Vitro. Biol Trace Elem Res 148, 178–181 (2012). https://doi.org/10.1007/s12011-012-9359-z

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  • DOI: https://doi.org/10.1007/s12011-012-9359-z

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