Biological Trace Element Research

, Volume 147, Issue 1–3, pp 142–148 | Cite as

Copper in Organic Proteinate or Inorganic Sulfate Form is Equally Bioavailable for Broiler Chicks Fed a Conventional Corn–Soybean Meal Diet

  • Songbai Liu
  • Lin Lu
  • Sufen Li
  • Jingjing Xie
  • Liyang Zhang
  • Runlian Wang
  • Xugang LuoEmail author


An experiment was conducted to investigate the bioavailability of organic copper (Cu) proteinate relative to inorganic Cu sulfate for broiler chicks fed a conventional corn–soybean meal basal diet. A total of 320 day-old Arbor Acres commercial male chicks were assigned to one of five treatments in a completely randomized design involving a 2 × 2 factorial arrangement with two levels of added Cu (125 or 250 mg Cu/kg) and two Cu sources (Cu proteinate and Cu sulfate) plus a control with no added Cu for an experimental phase of 42 days. Plasma and liver tissue samples were collected at both 21 and 42 days of age, and bile samples were also obtained at 42 days of age for Cu analyses. The Cu concentrations in liver and bile increased linearly (P < 0.001) on both days 21 and 42 as dietary Cu levels increased. No significant (P > 0.17) linear regression relationships were observed between plasma Cu concentrations on days 21 and 42 or log10 liver Cu concentration on day 21 and daily analyzed Cu intake. Therefore, based on the slope ratios from multiple linear regressions of log10 liver and bile Cu concentrations with daily analyzed Cu intake on day 42, when Cu sulfate was set as 100%, the estimated relative bioavailability values of Cu proteinate were 78.8% for log10 liver Cu concentration and 79.3% for log10 bile Cu concentration, respectively. There was no significant (P > 0.08) difference in bioavailability between Cu proteinate and Cu sulfate for broilers chicks in this experiment.


Cu proteinate Bioavailability Broiler chicks 



This work was supported by Alltech Inc. (3031 Catnip Hill Pike, Nicholasville, Kentucky 40356, USA), China Agriculture Research System (project no. CARS-42; Beijing, People’s Republic of China) and the Special Fund for Agro-scientific Research in the Public Interest (project no. 200903006; Beijing, People’s Republic of China).


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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Songbai Liu
    • 1
  • Lin Lu
    • 1
  • Sufen Li
    • 1
    • 2
  • Jingjing Xie
    • 1
  • Liyang Zhang
    • 1
  • Runlian Wang
    • 3
  • Xugang Luo
    • 1
    Email author
  1. 1.Mineral Nutrition Research Division, Institute of Animal ScienceChinese Academy of Agricultural SciencesBeijingPeoples’ Republic of China
  2. 2.Department of Animal ScienceHebei Normal University of Science and TechnologyQinhuangdaoPeoples’ Republic of China
  3. 3.Department of Animal ScienceGuangdong Ocean UniversityZhanjiangPeoples’ Republic of China

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