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Effect of Selenium on Connexin Expression, Angiogenesis, and Antioxidant Status in Diabetic Wound Healing

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Abstract

This study was done to analyze the effect of selenium on antioxidant status and expression of different connexins in diabetic wound healing. The levels of vascular endothelial growth factor, superoxide dismutase, lipid peroxide, and connexins were analyzed in wound tissues taken from diabetic and non-diabetic mice before and after sodium selenite administration. The mRNA transcript levels of Cx 26, 30.3, 31, 31.1, and 43 were significantly elevated in diabetic wounds as compared to the non-diabetic wounds. After selenium administration, the expression of connexins along with serum glucose decreases more significantly in diabetic wounds as compared to non-diabetic wounds. In diabetic wounds, the low levels of vascular endothelial growth factor and extracellular superoxide dismutase were restored to normal level following selenium administration. The lipid peroxidation decreased significantly in diabetic mice post-selenium administration. The histopathological analysis revealed that administration of selenium improves angiogenesis at the wound site. The results of this study demonstrate that selenium, acting as an essential component of the antioxidant system, normalizes the antioxidant status, and as an insulin mimetic compound, downregulates connexin expressions and induces angiogenesis. Together, these effects of selenium accelerate wound healing in diabetic conditions.

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Acknowledgement

We are thankful to Dr. Luiz Anastacio Alves, Department of Immunology, Oswaldo Cruz Institute, Brazil for his kind consent to use primers. The financial assistance extended by Banaras Hindu University is greatly acknowledged. S. Bajpai is highly thankful to C.S.I.R. for providing senior research fellowship (S.R.F.). Authors M. Mishra and H. Kumar are thankful to UGC for financial support as Dr. D.S. Kothari Postdoctoral fellowship {4-2/2006(BSR)/13-334/2010(BSR)} and S.R.F., respectively.

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Authors and Affiliations

  1. Department of Biochemistry, Faculty of Science, Banaras Hindu University, Varanasi, 221005, India

    Surabhi Bajpai, Manish Mishra, Haushila Prasad Pandey & Rakesh Kumar Singh

  2. Department of Medicine, Institute of Medical Sciences, Banaras Hindu University, Varanasi, 221005, India

    Manish Mishra, Hemant Kumar & Kamlakar Tripathi

  3. Centre of Experimental Medicine and Surgery, Institute of Medical Sciences, Banaras Hindu University, Varanasi, 221005, India

    Santosh Kumar Singh

Authors
  1. Surabhi Bajpai
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  2. Manish Mishra
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  3. Hemant Kumar
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  4. Kamlakar Tripathi
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  5. Santosh Kumar Singh
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  6. Haushila Prasad Pandey
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  7. Rakesh Kumar Singh
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Corresponding author

Correspondence to Rakesh Kumar Singh.

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Surabhi Bajpai and Manish Mishra contributed equally for this study.

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Bajpai, S., Mishra, M., Kumar, H. et al. Effect of Selenium on Connexin Expression, Angiogenesis, and Antioxidant Status in Diabetic Wound Healing. Biol Trace Elem Res 144, 327–338 (2011). https://doi.org/10.1007/s12011-011-9097-7

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  • DOI: https://doi.org/10.1007/s12011-011-9097-7

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