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Effect of Copper on the Expression of TGF-β in Incubated Chondrocytes of Newborn Pigs

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Abstract

This experiment was conducted to measure the effect of copper supplementation on TGF-β gene expression in chondrocytes of newborn pigs. Chondrocytes were cultured in media containing 15% fetal calf serum supplemented with 0, 15.6, 31.2, and 62.5 μmol/L copper in 90-mm culture plate. Total RNA was isolated from chondrocytes, and TGF-β cDNA was synthesized, amplified, and sequenced. The expression level of TGF-β was examined by reverse transcription polymerase chain reaction. The results showed that the sequence of the cloned TGF-β gene was 99.4% identical to that in GenBank. The expression of TGF-β increased in culture media added with final concentration of 15.6, 31.2, and 62.5 μmol/L copper. In this study, the optimal copper concentration and optimal culture time for the highest level of TGF-β expression were 31.2 μmol/L and 48 h, respectively.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (Grant nos. 31072178, 30600441, 30972212, and 30871897), by the Doctoral Program Foundation of Institutions of Higher Education of China (Grant no. 20070183156), and by the Science Frontier and Cross-disciplinary Innovation Project of Jilin University (Grant no. 200903022).

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Authors and Affiliations

  1. College of Animal Science and Veterinary Medicine, Jilin University, 5333 Xi’an Road, Changchun, 130062, China

    Xiaoyan Zhu, Jianguo Wang, Guanghong Xie, Haihua Feng, Xiaobing Li, Lei Liu, Xiaoxu Wang, Dongna Li, Zhaoxi Liu, Zhe Wang & Guowen Liu

  2. Institute of Animal Science and Technology, Tarim University of Agricultural Reclamation, Alar, Xinjiang, 843300, China

    Jian Qian

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  1. Xiaoyan Zhu
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  2. Jianguo Wang
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  5. Xiaobing Li
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  6. Lei Liu
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  10. Jian Qian
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  11. Zhe Wang
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Corresponding authors

Correspondence to Zhe Wang or Guowen Liu.

Additional information

Xiaoyan Zhu, Jianguo Wang, Haihua Feng, and Guanghong Xie contributed equally to this study.

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Zhu, X., Wang, J., Xie, G. et al. Effect of Copper on the Expression of TGF-β in Incubated Chondrocytes of Newborn Pigs. Biol Trace Elem Res 143, 1461–1469 (2011). https://doi.org/10.1007/s12011-011-8966-4

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  • DOI: https://doi.org/10.1007/s12011-011-8966-4

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