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Dietary Excess Vanadium Induces Lesions and Changes of Cell Cycle of Spleen in Broilers

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Abstract

The purpose of this 42-day study was to investigate the effects of dietary excess vanadium on spleen growth and lesions by determining morphological changes and cell cycle of spleen. Four hundred twenty 1-day-old avian broilers were divided into six groups and fed on a corn–soybean basal diet as control diet or the same diet amended to contain 5, 15, 30, 45, 60 ppm of vanadium supplied as ammonium metavanadate. When compared with that of control group, the relative weight of spleen was significantly raised in 5- and 15-ppm groups, but depressed in 45- and 60-ppm groups. The gross lesions of spleen showed obvious atrophy with decreased volume and pale color in 45- and 60-ppm groups. Histopathologically, lymphocytes in splenic corpuscle and periarterial lymphatic sheath were variously decreased in number in 30-, 45-, and 60-ppm groups. The percentage of static phase (G0/G1) was significantly decreased, and the percentage of synthesis period (S) phase and the proliferating index (PI) were significantly increased in 5- and 15-ppm groups. The percentage of G0/G1 phase was significantly increased, and the percentage of mitotic phase (G2 + M), S phase, and PI significantly decreased in 45- and 60-ppm groups. These results suggested that dietary excess vanadium (45 and 60 ppm) could inhibit growth of spleen and induce lesions in spleen in chicken.

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Acknowledgment

The study was supported by the program for Changjiang scholars and innovative research team in university (IRT 0848) and the key program of Education Department and Scientific Department of Sichuan Province (09ZZ017).

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Authors and Affiliations

  1. Key Laboratory of Animal Diseases and Environmental Hazards of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Ya’an, Sichuan, 625014, China

    Wei Cui, Hengmin Cui, Xi Peng, Jing Fang, Zhicai Zuo, Xiaodong Liu & Bangyuan Wu

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  1. Wei Cui
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  2. Hengmin Cui
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  3. Xi Peng
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  4. Jing Fang
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  5. Zhicai Zuo
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  6. Xiaodong Liu
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  7. Bangyuan Wu
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Correspondence to Hengmin Cui.

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Cui, W., Cui, H., Peng, X. et al. Dietary Excess Vanadium Induces Lesions and Changes of Cell Cycle of Spleen in Broilers. Biol Trace Elem Res 143, 949–956 (2011). https://doi.org/10.1007/s12011-010-8938-0

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  • DOI: https://doi.org/10.1007/s12011-010-8938-0

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