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Study on Zn Relative Concentration and State in Sheep Duodenum by XAFS

Biological Trace Element Research volume 143, pages 240–250 (2011)Cite this article

Abstract

Synchrotron-based X-ray absorption fine structure (XAFS) spectroscopy is not widely used in animal science. The objective of this study was to employ the XAFS technique to determine changes in zinc absorption and concentrations in the sheep interstinal sac using different zinc sources. Forty-eight sheep were slaughtered and their duodena were dissected. The duodena were randomly assigned to six zinc sources (ZnO, ZnSO4, ZnMet, ZnLys, ZnSO4 + methionine, ZnSO4 + lysine). Ten centimeters of duodenal sac midpiece was incubated for 40 min in vitro using the everted intestinal sac technique in culture medium containing zinc from different sources. The amount of zinc present was normalized to 4 mg. XAFS was used to analyze the relative concentration and oxidation state of zinc, and atomic absorption spectrometry (AAS) was used to verify zinc concentration. The results showed that, for increasing zinc concentrations, organic zinc was a better source than inorganic zinc. In addition, the zinc concentration achieved using ZnMet was higher than that for ZnO and ZnSO4 (P < 0.05) as measured by atomic absorption spectroscopy. The results using XAFS were consistent with that of AAS. The states of organic zinc and inorganic zinc were identical after being incubated for 40 min. As observed in these experiments, organic zinc was more easily absorbed than inorganic zinc. Our data demonstrate that organic zinc was dissociated into ions and then absorbed as inorganic zinc. In our experiments, we are the first investigators to use XAFS spectroscopy to determine zinc absorption in the sheep duodenal wall. We observed reduced absorption of inorganic zinc in the presence of methionine or lysine. Taken together, we postulate that the optimal molar ratio of inorganic zinc and ligand requires further study.

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Acknowledgments

This study was supported by the Returned Overseas Fund of Heilongjiang, People’s Republic of China (project number LC08C35). This work was performed in Shanghai Synchrotron Radiation Facility (SSRF), BL14W1 XAFS experimental station. We thank the staff of the SSRF for assistance with this study.

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Affiliations

  1. Animal Nutrition Institute, Northeast Agricultural University, 150030, Harbin, Heilongjiang, China

    Meixia Sui, Haixia Liu, Yanfeng Ren & Dasen Liu

  2. College of Science, Northeast Agricultural University, 150030, Harbin, Heilongjiang, China

    Dasen Liu

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  1. Meixia Sui
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  2. Haixia Liu
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  3. Yanfeng Ren
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  4. Dasen Liu
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Correspondence to Dasen Liu.

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Sui, M., Liu, H., Ren, Y. et al. Study on Zn Relative Concentration and State in Sheep Duodenum by XAFS. Biol Trace Elem Res 143, 240–250 (2011). https://doi.org/10.1007/s12011-010-8843-6

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  • DOI: https://doi.org/10.1007/s12011-010-8843-6

Keywords

  • XAFS
  • Duodenum
  • Zinc
  • Relative concentration
  • State