Abstract
The purpose of this study was to evaluate the effects of vanadium absorbed by Coprinus comatus (VACC) treatment on bone in streptozotocin (STZ)-induced diabetic rats. Forty-five Wistar female rats used were divided into three groups: (1) normal rats (control), (2) diabetic rats, and (3) diabetic rats treated with VACC. Normal and diabetic rats were given physiological saline, and VACC-treated rats were administered VACC intragastrically at doses of 0.18 mg vanadium/kg body weight once daily. Treatments were performed over a 12-week period. At sacrifice, one tibia and one femur were removed, subjected to micro computed tomography (micro-CT) for determination of trabecular bone structure, and then processed for histomorphometry to assess bone turnover. Another femoral was used for mechanical testing. In addition, bone samples were collected to evaluate the content of mineral substances in bones. Treatment with VACC increased trabecular bone volume fraction in diabetic rats. Vanadium-treated animals had significant increases in ultimate load, trabecular thickness, and osteoblast surface. However, vanadium treatment did not seem to affect bone stiffness, bone energy absorption, trabecular separation, and osteoclast number. P levels in the femurs of diabetic rats treated with VACC were significantly higher than those of diabetic animals. Ca levels in diabetic and diabetic rats treated with vanadium showed no obvious changes. In conclusion, our results provide an important proof of concept that VACC may represent a powerful approach to treating or reversing diabetic osteopathy in humans.
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References
Tripathi BK, Srivastava AK (2006) Diabetes mellitus: complications and therapeutics. Med Sci Monit 12(7):RA130–RA147
Goodman WG, Hori MT (1984) Diminished bone formation in experimental diabetes. Relationship to osteoid maturation and mineralization. Diabetes 33:825–831
Strotmeyer ES, Cauley JA (2007) Diabetes mellitus, bone mineral density, and fracture risk. Curr Opin Endocrinol Diabetes Obes 14(6):429–435
Macey LR, Kana SM, Jingushi S, Terek RM, Borretos J, Bolander ME (1989) Defects of early fracture-healing in experimental diabetes. J Bone Jt Surg Am 71:722–733
Katayama Y, Akatsu T, Yamamoto M, Kugai N, Nagata N (1996) Role of nonenzymatic glycosylation of type I collagen in diabetic osteopenia. J Bone Miner Res 11:931–937
Levin ME, Boisseau VC, Avioli LV (1976) Effects of diabetes mellitus on bone mass in juvenile and adult onset diabetics. N Engl J Med 294:241–245
McNair P, Madsbad C, Christiansen C, Faber OK, Transbol I, Binder C (1978) Osteopenia of insulin treated diabetes mellitus: its relation to age of onset, sex and duration of disease. Diabetologia 15:87–93
Rosenbloom AL, Lezotte DC, Weber T, Gudat J, Heller DR, Weber ML, Klein S, Kennedy BB (1977) Diminution of bone mass in childhood diabetes. Diabetes 26:1052–1055
Santiago JV, McAlister WH, Ratzan SK, Bussman Y, Haymond MW, Shackelford G, Weldon VV (1977) Decreased cortical thickness and osteopenia in children with diabetes mellitus. Clin Endocrinol Metab 43:845–848
Balint E, Szabo P, Marshall CF, Sprague SM (2001) Glucose-induced inhibition of in vitro bone mineralization. Bone 28:21–28
Verhaeghe J, Suiker AM, Einhorn TA, Geusens P, Visser WJ, Van Herck E, Van Bree R, Magitsky S, Bouillon R (1994) Brittle bones in spontaneously diabetic female rats cannot be predicted by bone mineral measurements: studies in diabetic and ovariectomized rats. J Bone Miner Res 9:1657–1667
Paul RG, Bailey AJ (1996) Glycation of collagen: the basis of its central role in the late complications of ageing and diabetes. Int J Biochem Cell Biol 28:1297–1310
Srivastava AK (2000) Anti-diabetic and toxic effects of vanadium compounds. Mol Cell Biochem 206:177–182
Tsiani E, Fantus IG (1997) Vanadium compounds. Biological actions and potential as pharmaocological agents. Trends Endocrinol Metabol 8:51–58
Shechter Y (1990) Insulin-mimetic effects of vanadate. Possible implications for future treatment of diabetes. Diabetes 39:1–5
Domingo JL (2002) Vanadium and tungsten derivatives as antidiabetic agents: a review of their toxic effects. Biol Trace Elem Res 88:97–112
McNeill JH, Yuen VG, Hoveyda HR (1992) Bis(maltolato)oxovanadium(IV) is a potent insulin mimic. J Med Chem35(8): 1489–1491
Sakurai H, Fujii K, Watanabe H (1995) Orally active and long-term acting insulin-mimetic vanadyl complex: bis (picolinato) oxovanadium (IV). Biochem Biophys Res Commun 214:1095–1101
Swanston-Flatt SK, Day C, Bailey CJ, Flatt PR (1989) Evaluation of traditional plant treatments for diabetes: studies in streptozotocin diabetic mice. Acra Diaberologiu Larinu 26:51–55
Kiho T, Tsujimura Y, Sakushima M, Usui S, Ukai S (1994) Polysaccharides in fungi: XXXIII. Hypoglycemic activity of an acidic polysaccharide (AC) from Tremella fuciformis. Yakugaku Zasshi 114:308–315, in Japanese
Kiho T, Sobue S, Ukai S (1994) Structural features and hypoglycemic activities of two polysaccharides from a hot-water extract of Agrocybe cylindracea. Carbohydr Res 251:81–87
Kalac P, Niznamska M, Bevilaqua D, Staskova I (1996) Concentrations of mercury, copper, cadmium and lead in fruiting bodies of edible mushrooms in the vicinity of a mercury smelter and a copper smelter. Sci Total Environ 177:251–258
Kalac P, Svoboda L (2000) A review of trace element concentrations in edible mushrooms. Food Chem 69:273–281
Han C, Yuan J, Wang Y (2006) Hypoglycemic activity of fermented mushroom of Coprinus comatus rich in vanadium. J Trace Elem Med Biol 20(3):191–196
Verhulp E, van Rietbergen B, Huiskes R (2004) A three-dimensional digital image correlation technique for strain measurements in microstructures. J Biomech 37:1313–1320
Goto A, Tsukamoto I (2003) Increase in tartrate-resistant acid phosphatase of bone at the early stage of ascorbic acid deficiency in the ascorbate-requiring Osteogenic Disorder Shionogi (ODS) rat. Calcif Tissue Int 73:180–185
Lu B, Ennis D, Lai R, Bogdanovic E (2001) Enhanced sensitivity of insulin-resistant adipocytes to vanadate is associated with oxidative stress and decreased reduction of vanadate (+5) to vanadyl (+4). J Biol Chem 276:35589–35598
Semiz S, Orvig C, McNeill JH (2002) Effects of diabetes, vanadium, and insulin on glycogen synthase activation in Wistar rats. Mol Cell Biochem 231:23–35
Goldfine AB, Simonson DC, Folli F (1995) In vivo and in vitro studies of vanadate in human and rodent diabetes mellitus. Mol Cell Biochem 153:217–231
Forst T, Pfutzner A, Kann P, Schehler B, Lobmann R, Schafer H, Andreas J, Bockisch A, Beyer J (1995) Peripheral osteopenia in adult patients with insulin-dependent diabetes mellitus. Diabetes Med 12:874–879
Buysschaert M, Cauwe F, Jamart J, Brichant C, De Coster P, Magnan A, Donckier J (1992) Proximal femur density in type 1 and 2 diabetic patients. Diabetes Metab 18:32–37
Hui SL, Epstein S, Johnston CC Jr (1985) A prospective study of bone mass in patients with type I diabetes. J Clin Endocrinol Metab 60:74–80
Miazgowski T, Czekalski S (1998) A 2-year follow-up study on bone mineral density and markers of bone turnover in patients with long-standing insulin-dependent diabetes mellitus. Osteoporos Int 8:399–403
Olmos JM, Perez-Castrillon JL, Garcia MT, Garrido JC, Amado JA, Gonzalez-Macias J (1994) Bone densitometry and biochemical bone remodeling markers in type 1 diabetes mellitus. Bone Miner 26:1–8
Poucheret P, Verma S, Grynpas MD, McNeill JH (1998) Vanadium and diabetes. Mol Cell Biochem 188:73–80
Facchini DM, Yuen VG, Battell ML, McNeill JH, Grynpas MD (2006) The effects of vanadium treatment on bone in diabetic and non-diabetic rats. Bone 38:368–377
Verhaeghe J, van Herck E, Visser WJ, Suiker AM, Thomasset M, Einhorn TA, Faierman E, Bouillon R (1990) Bone and mineral metabolism in BB rats with longterm diabetes. Decreased bone turnover and osteoporosis. Diabetes 39:477–482
Einhorn TA, Boskey AL, Gundberg CM, Vigorita VJ, Devlin VJ, Beyer MM (1988) The mineral and mechanical properties of bone in chronic experimental diabetes. J Orthop Res 6:317–323
Hou JC, Zernicke RF, Barnard RJ (1991) Experimental diabetes, insulin treatment, and femoral neck morphology and biomechanics in rats. Clin Orthop Relat Res 264:278–285
Pothuaud L, Rietbergen BV, Mosekilde L et al (2002) Combination of topological parameters and bone volume fraction better predicts the mechanical properties of trabecular bone. Bone 35:1091–1099
Ulrich D, Rietbergen BV, Laib A, Rüegsegger P (1999) The ability of three-dimensional structure indices to reflect mechanical aspects of trabecular bone. Bone 25:55–60
Bain S, Ramamurthy NS, Impeduglia T, Scolman S, Golub LM (1997) Tetracycline prevents cancellous bone loss and maintains near-normal rates of bone formation in streptozotocin diabetic rats. Bone 21:147–153
Thomas DM, Hards DK, Rogers SD, Ng KW, Best JD (1996) Insulin receptor expression in bone. J Bone Miner Res 11:1312–1320
Cornish J, Callon KE, Reid IR (1996) Insulin increases histomorphometric indices of bone formation in vivo. Calcif Tissue Int 59:492–495
Fukui K, Fujisawa Y, OhyaNishiguchi H, Kamada H, Sakurai H (1999) In vivo coordination structural changes of a potent insulin-mimetic agent, bis(picolinato)oxovanadium(IV), studied by electron spin–echo envelope modulation spectroscopy. J Inorg Biochem 77:215–224
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Pei, Y., Fu, Q. The Effects of Vanadium (V) Absorbed by Coprinus comatus on Bone in Streptozotocin-induced Diabetic Rats. Biol Trace Elem Res 142, 748–759 (2011). https://doi.org/10.1007/s12011-010-8825-8
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DOI: https://doi.org/10.1007/s12011-010-8825-8