Biological Trace Element Research

, Volume 141, Issue 1–3, pp 91–95 | Cite as

Electron Microscopic (Energy Dispersive X-ray Analysis) Study of Human Male Reproductive Organs and Semen

  • K. P. Skandhan
  • B. Sumangala
  • S. Amith
  • K. P. S. Avni


Recently, technological advancement helped to improve our knowledge on trace elements in human male reproductive organs and its secretion, semen. In this study, employing energy dispersive x-ray analysis facilities on electron microscope, presence of different elements in human male reproductive organs-—testis, epididymis, caput, corpus and cauda, prostate gland, seminal vesicle, Cowper’s gland and vas deferens—seminal plasma and spermatozoa pellet was studied. Several elements were observed. Gold was one among them that was present in seminal plasma and spermatozoa. It was also present in epididymis caput. Authors consider epididymis caput as the source of gold in semen.


Electron microscope EDAX Human semen elements Gold 


  1. 1.
    Mann T, Mann L (1981) Male reproductive function and semen. Springer Verlag, BerlinGoogle Scholar
  2. 2.
    Shivaji S, Schelt K, Bhargava PM (1989) Protein of seminal plasma. Wiley Interscience, New YorkGoogle Scholar
  3. 3.
    Skandhan KP (1981) Gold in human semen. Andrologia 13:78–81PubMedCrossRefGoogle Scholar
  4. 4.
    Skandhan KP, Abraham KC (1984) Presence of several elements in seminal plasma of normal and of different pathological conditions. Andrologia 16:587–588PubMedCrossRefGoogle Scholar
  5. 5.
    Skandhan KP, Amith S, Avni KPS (2009) X-ray diffraction study on human male reproductive system and semen. Urologia 76:198–202PubMedGoogle Scholar
  6. 6.
    Skandhan KP (1981) Gold in normal and pathological human seminal plasma. Israel J Med Sci 17:769–770Google Scholar
  7. 7.
    Mann T (1964) The biochemistry of semen and of the male reproductive tract. Methuen and Company Ltd, LondonGoogle Scholar
  8. 8.
    Skandhan KP, Mazumdar BN (1985) LE Zinc dans le plasma seminal. Revue Franc Obstet Gynecol 80:894–898Google Scholar
  9. 9.
    Skandhan KP (1992) Review on copper in male reproduction and contraception. Rev Fr Obstet Gynecol 87:594–598Google Scholar
  10. 10.
    Blean GD, Bonsquet M, Jean VY et al (1977) Selenium in human semen. Fertil Steril 31:246Google Scholar
  11. 11.
    Skandhan KP, Makada M, Amith S (2006) Level of cadmium, chromium, manganese and lead in normal and pathological human seminal plasma. Urologia 72:461–464Google Scholar
  12. 12.
    Pleban PA, Mei DS (1983) Trace elements in human seminal plasma and spermatozoa. Clin Chim Acta 133:43–50PubMedCrossRefGoogle Scholar
  13. 13.
    Skandhan KP, Pandya CB (1986) Direct couple plasma emission spectroscopic study on human seminal plasma. Adv Contr Dely Syst 2:256–257Google Scholar
  14. 14.
    Skandhan KP, Sumangala B, Metha YB et al (2010) Level of gold in normal and pathological semen. Gynecol Obstet Biol Repord (in press)Google Scholar
  15. 15.
    Skandhan KP, Sumangala B, Metha YB et al (1992) Histological demonstration of gold in male genital system of rat. Urologia 59:75–76Google Scholar
  16. 16.
    Lindholmer C, Eliasson R (1974) In vitro release and uptake of zinc and magnesium by human spermatozoa. Int J Fertil 19:56–62PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • K. P. Skandhan
    • 1
    • 3
  • B. Sumangala
    • 2
    • 4
  • S. Amith
    • 5
  • K. P. S. Avni
    • 6
  1. 1.Department of PhysiologyGovernment Medical CollageSuratIndia
  2. 2.Department of PathologyGovernment Medical CollageSuratIndia
  3. 3.Department of PhysiologySree Narayana Institute of Medical SciencesErnakulamIndia
  4. 4.Department of PathologySree Narayana Institute of Medical SciencesErnakulamIndia
  5. 5.Mercy HospitalPittsburghUSA
  6. 6.Department of RadiologyMalabar institute of Medical SciencesCalicutIndia

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