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Formulation of Nutrient Medium for In Vitro Somatic Embryo Induction in Plantago ovata Forsk

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Abstract

A nutrient medium has been formulated by altering the macro- and micro-elemental concentration in the culture medium for in vitro somatic embryo induction of economically important medicinal plant Plantago ovata Forsk .A comparison was made between induced embryos with normal embryos (produced in Murashige and Skoog (MS) medium) to observe frequency of embryo induction and also to determine regeneration efficiency. In the present investigation, three different media have been formulated. Among them, FM3 (formulated media, treatment 3) was the most suitable for increasing the frequency of somatic embryo production and regeneration of P. ovata Forsk. Better result was obtained using formulated medium than with MS medium.

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Acknowledgment

The authors express sincere gratitude to UGC-DAE CRS Kolkata chapter for the facility of ED-XRF.

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  1. Department of Biophysics, Molecular Biology and Bioinformatics, University of Calcutta, 92, APC Road, Kolkata, 700009, India

    Priyanka Saha, Subhendu Bandyopadhyay & Sarmistha Sen Raychaudhuri

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  1. Priyanka Saha
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  2. Subhendu Bandyopadhyay
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Correspondence to Sarmistha Sen Raychaudhuri.

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Saha, P., Bandyopadhyay, S. & Raychaudhuri, S.S. Formulation of Nutrient Medium for In Vitro Somatic Embryo Induction in Plantago ovata Forsk. Biol Trace Elem Res 140, 225–243 (2011). https://doi.org/10.1007/s12011-010-8684-3

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