Abstract
The effects of administration of oxidized rapeseed oil and α-lipoic acid on activities of hepatic antioxidant enzymes and lipid peroxidation were studied in laboratory rats. There was an increase of the activities of superoxide dismutase, glutathione peroxidase, glutathione reductase, and glucose-6-phosphate dehydrogenase in rats fed a high fat diet to which 10% oxidized oil was added. Administration of α-lipoic acid resulted in a decrease of the activities of these enzymes. Addition of oxidized oil also resulted in increased production of oxygen radicals, evidenced by elevated malondialdehyde production. Such effect was counteracted by administration of α-lipoic acid.
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References
Thomas-Moya E, Gianotti M, Proenza AM et al (2007) Paraoxonase 1 response to a high-fat diet: gender differences in the factors involved. Mol Med 13(3–4):203–209
Christon RA (2003) Mechanism of action of dietary fatty acids in regulating the activation of vascular endothelial cells during atherogenesis. Nutr Rev 61:272–279
Thijssen MA, Mensink RP (2005) Fatty acids and atherosclerotic risk. Handb Exp Pharmacol 170:165–194
Storlien LH (1996) Dietary fats and insulin action. Diabetologia 39:621–631
Zalejska-Fiolka J (2000) Antioxidative properties of alpha-tocopherol, methionine and selenomethionine in olive oil. La Rivista Italiana Delie Sostanze Grasse LXXVII:543–547
Huang CY, Chen LH, Osio Y et al (1994) Effects of diet composition on liver antioxidant defence and detoxification enzymes in mice with murine AIDS. Nutr Res 14:1841–1851
Bok SH, Shin YW, Bae KW et al (2000) Effects of naringin and lovastatin on plasma and hepatic lipids in high-fat and high-cholesterol fed rats. Nutr Res 20:1007–1015
Bustamante J, Lodge JK, Marcocci L et al (1998) Alpha-lipoic acid in liver metabolism and disease. Free Radic Biol Med 24(6):1023–1039
Bilska A, Włodek L (2005) Lipoic acid—the drug of the future? Pharmacol Rep 57(5):570–577
Arivazhagan P, Ramanathan K, Panneerselvam C (2001) Effect of dl-alpha-lipoic acid on mitochondrial enzymes in aged rats. Chem Biol Interact 138(2):189–198
Polish Standardizing Committee—animal and vegetable oils and fats—determination of peroxide value (pp. 3–7). Warsaw, 1996: Norm. Alfa–Wero. Polish Standardizing Committee—animal and vegetable oils and fats—determination of iodine value (pp. 3–7). Warsaw, 1998: Norm. Alfa–Wero
Lowry OM, Rosenbrough NJ, Farr AL et al (1951) Protein measurement with Folin phenol reagent. J Biol Chem 193:265–275
Oyanagui Y (1984) Reevaluation of assay methods and establishment of kit for superoxide dismutase activity. Anal Biochem 142:290–296
Aebi H (1984) Catalase in vitro. Methods Enzymol 105:121–126
Paglia D, Valentine W (1967) Studies on the quantities and quantitative characterisation of erythrocyte glutathione peroxidase. J Lab Clin 70:158–169
Ohkawa H, Ohishi N, Yagi K (1973) Assay for peroxidases in animal tissues by thiobarbituric acid reaction. Anal Biochem 95:351–358
Richterich R (1971) Clinical chemistry. PZWL, Warsaw, pp 359–360
Warner K (1999) Impact of high-temperature food processing on fats and oils. Adv Exp Med Biol 459:67–77
Zalejska-Fiolka J, Kasperczyk S, Kasperczyk A et al (2004) The influence of oxidant vegetable oil and garlic extract upon the development of experimental atherosclerosis in rabbits. Bull Vet Inst Pulawy 48:453–459
Zalejska-Fiolka J (2001) The influence of garlic extract on oxidation of edible oils. Riv Ital Sostanze Grasse 6:343–346
Zalejska-Fiolka J (2002) The influence of garlic extract on the oxidation process of edible oils. Part II. Riv Ital Sostanze Grasse 10:365–368
Zalejska-Fiolka J, Kasperczyk A, Kasperczyk S et al (2007) Effect of garlic supplementation on erythrocytes antioxidant parameters, lipid peroxidation, and atherosclerotic plaque formation process in oxidized oil-fed rabbits. Biol Trace Elem Res 120(1–3):195–204
Roberts UA, Fisher CL, Redford SM et al (1991) Mechanism and atomic structure of superoxide dismutase. Free Radic Res Commun 12-13:269–278
Meister A (1988) Glutathione metabolism and its selective modification. J Biol Chem 263:17205–17208
Narasimhamurthy K, Raina PL (1999) Long-term feeding effect of thermally oxidised oils on antioxidant enzymes in rats. Indian J Exp Biol 37(10):1042–1045
Chen HW, Tsai CW, Yang JJ et al (2003) The combined effects of garlic oil and fish oil on the hepatic antioxidant and drug-metabolizing enzymes of rats. Br J Nutr 89(2):189–200
Sahin M, Sağdiç G, Elmas O et al (2006) Effect of chronic restraint stress and alpha-lipoic acid on lipid peroxidation and antioxidant enzyme activities in rat peripheral organs. Pharmacol Res 54(3):247–252
Purushothama S, Ramachandran HD et al (2003) Long-term feeding effects of heated and fried oils on hepatic antioxidant enzymes, absorption and excretion of fat in rats. Mol Cell Biochem 247(1–2):95–99
Park KG, Min AK, Koh EH et al (2008) Alpha-lipoic acid decreases hepatic lipogenesis through adenosine monophosphate-activated protein kinase (AMPK)-dependent and AMPK-independent pathways. Hepatology 48(5):1477–1486
Vanasco V, Cimolai MC, Evelson P et al (2008) The oxidative stress and the mitochondrial dysfunction caused by endotoxemia are prevented by alpha-lipoic acid. Free Radic Res 42(9):815–823
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Zalejska-Fiolka, J., Wielkoszyński, T., Kasperczyk, S. et al. Effects of Oxidized Cooking Oil and α-Lipoic Acid on Liver Antioxidants: Enzyme Activities and Lipid Peroxidation in Rats Fed a High Fat Diet. Biol Trace Elem Res 138, 272–281 (2010). https://doi.org/10.1007/s12011-010-8628-y
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DOI: https://doi.org/10.1007/s12011-010-8628-y