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The Organic Selenium Compound Selenomethionine Modulates Bleomycin-Induced DNA Damage and Repair in Human Leukocytes

Biological Trace Element Research volume 133, pages 12–19 (2010)Cite this article

Abstract

The objective of this work was to evaluate the effects of selenomethionine (SeMet) on the induction, repair, and persistence of DNA damage in human leukocytes challenged with bleomycin (BLM). Comet assay was used to determine DNA strand breaks and hOGG1 for the specific recognition of oxidative damage. Leukocytes were (A) stimulated with phytohemagglutinin, (B) damaged with BLM, and (C) incubated to allow DNA repair. Comet assay was performed after each phase. SeMet (50 μM) was supplemented either during phase A, B, or C, or AB, or ABC. Treatment with SeMet decreased BLM-induced stand breaks when added during phase AB. Results obtained after the repair period indicate that SeMet favors repair of DNA damage especially when applied during phase AB. The comparison between DNA damage before and after repair showed that BLM-induced damage was repaired better in the presence of SeMet. Our results showed antigenotoxic effect of SeMet on BLM-induced DNA and also on repair and persistence of this damage when applied before and simultaneously with BLM.

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Acknowledgements

This work was funded by a grant from the Xunta de Galicia (INCITE08PXIB106155PR). V. Valdiglesias was supported by a fellowship from the University of A Coruña.

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Authors and Affiliations

  1. Toxicology Unit, Department of Psychobiology, University of A Coruña, Edificio de Servicios Centrales de Investigación, Campus Elviña s/n, 15071, A Coruña, Spain

    Blanca Laffon, Vanessa Valdiglesias & Eduardo Pásaro

  2. Department of Cell and Molecular Biology, Faculty of Sciences, University of A Coruña, Campus A Zapateira s/n, 15071, A Coruña, Spain

    Vanessa Valdiglesias & Josefina Méndez

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  1. Blanca Laffon
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  2. Vanessa Valdiglesias
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  3. Eduardo Pásaro
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  4. Josefina Méndez
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Correspondence to Blanca Laffon.

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Laffon, B., Valdiglesias, V., Pásaro, E. et al. The Organic Selenium Compound Selenomethionine Modulates Bleomycin-Induced DNA Damage and Repair in Human Leukocytes. Biol Trace Elem Res 133, 12–19 (2010). https://doi.org/10.1007/s12011-009-8407-9

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  • DOI: https://doi.org/10.1007/s12011-009-8407-9

Keywords

  • Selenomethionine
  • Bleomycin
  • Comet assay
  • Oxidative damage
  • Antigenotoxicity