To determine the content of total iron (TFe) and heme iron (HeFe) in major cuts of meat and principal viscera of bovine origin. 55Fe (30 mCi) was injected into two 4-month-old calves. Triplicate samples of the 12 basic American cuts of meat and major viscera were obtained from each specimen. Samples were acid digested and their iron content was read by atomic absorption spectrophotometry. Duplicate samples of the basic cuts of meat and major viscera were analyzed to determine the concentration of 55Fe using a double isotopic technique. The mean and standard deviation of TFe for all cuts was 1.4 ± 0.3 mg/100 g of meat. The mean TFe for organs was (per mg/100 g): 0.9 ± 0.1 brain, 3.0 ± 0.05 kidney, 3.2 ± 0.04 heart, 5.7 ± 0.2 lung, 6.0 ± 0.1 liver, and 31.2 ± 0.4 spleen. HeFe was 64% of TFe in meat and 72.8% in spleen, 53.8% in lung, 35.7% in brain, 35.0% in kidney, 27.3% in heart, and only 13.6% in liver. Blood contained 85.5% of the radioisotope and only 1.4% was found in muscle and 1.6% was found in viscera. Results suggest that bovine cuts of meat have a low variation in TFe and that HeFe comprises more than 60% of TFe.
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Millward D, Jackson A (2004) Protein/energy ration of current diets in developed and developing countries compared with a safe protein/energy ratio: implications for recommended protein and amino acid intakes. Public Health Nutr 7:387–405
DeMaeyer E, Adiels-Tegman M (1985) The prevalence of anaemia in the world. World Health Stat Q 38:302–316
Müller O, Krawinkel M (2005) Malnutrition and health in developing countries. Can Med J Ass 173:279–286
Moy RJ (2006) Prevalence, consequences and prevention of childhood nutritional iron deficiency: a child public health perspective. Clin Lab Haematol 28:291–298
Stoltzfus RJ (2003) Iron deficiency: global prevalence and consequences. Food Nutr Bull 24(4 Suppl):S99–S103
Darnton-Hill I, Webb P, Harvey P, Hunt J, Dalmiya N, Chopra M (2005) Micronutrient deficiencies and gender: social and economic costs. Am J Clin Nutr 81(5):1198–1205
Martínez-Torres C, Layrisse M (1971) Iron absorption from veal muscle. Am J Clin Nutr 24:531–540
Hallberg L, Björn-Rasmussen E (1972) Determination of iron absorption from whole diet. A new two-pool model using two radioiron isotopes given as haem and non-haem iron. Scand J Haematol 9:193–197
Cook J, Layrisse M, Martinez-Torres C, Walker R, Monsen E, Finch CA (1972) Food iron absorption measured by an extrinsic tag. J Clin Invest 51:805–815
Hallberg L (1981) Bioavailability of dietary iron in man. Annu Rev Nutr 1:123–147
Monsen E, Hallberg L, Layrisse M (1978) Estimation of available dietary iron. Am J Clin Nutr 31:134–141
Weintraub L, Weintein M, Huser H, Rafal S (1968) Absorption of hemoglobin iron: role of a heme-splitting substance in the intestinal mucosa. J Clin Invest 47:531–539
Raffin S, Woo C, Roost K, Price D, Schmid R (1974) Intestinal absorption of hemoglobin heme iron cleavage by mucosal heme oxygenase. J Clin Invest 54:1344–1352
Wheby M, Spyker D (1981) Hemoglobin iron absorption kinetics in iron deficient dog. Am J Clin Nutr 34:1686–1693
CONICYT (1994) Manual de normas de bioseguridad. In: CONICYT (Ed) Santiago, Chile. 140–190
Association of Official Analytical Chemists (1984) Official Methods of Analysis of the A.O.A.C. 14th edn. Arlington
Johnson R, Chen T, Muller W, Costello J, Romans K, Jones W (1988) Characterization of the muscles within the beef forequarter. J Food Sci 53:1247–1250
Jones S, Calkins K, Podany J, Sherrill R, Roeber A, Guru C, Chen V, Singh D, Selendic N, Kapetanovic and Gwartney K (2000) Bovine Myology. Available via DIALOG. http://animalscience.unl.edu/document.cgi?docID=144. Accessed 8 Dec 2008
NCBA (2000) Muscle profiling. National Cattlemen’s Beef Association, Denver
Rebouche C, Wilcox C, Widness J (2004) Microanalysis of non-heme iron in animal tissues. J Bioch Bioph Met 58:239–251
Eakins J, Brown D (1966) An improved method for the simultaneous determination of 55iron in blood by liquid scintillation counting. Int J Appl Radiat Isot 17:391–397
Jain, J (1986) Schalm’s veterinary hematology. In: Lea and Febiger (ed). 4th edn. Philadelphia
Institute of Medicine Food and Nutrition Board (2001) Dietary reference intakes for vitamin A, vitamin K, arsenic, boron, chromium, copper, iodine, iron, manganese, molybdenum, nickel, silicon, vanadium and zinc. National Academy Press, Washington, DC
Pennington J, Schoen S, Salmon G, Young B, Jonson R, Marts W (1995) Composition of core foods of the U.S. foods supply, 1982–1991. II. Calcium, magnesium, iron and zinc. J Food Compost Anal 8:129–169
Pennington J, Young BE, Wilson DB (1989) Nutritional elements in U.S. diets: results from the Total Diet Study, 1982 to 1986. J Am Diet Assoc 89:659–664
Olivares M, Pizarro F, De Pablo S, Araya M, Uauy R (2004) Iron, zinc and copper: contents in common Chilean foods and daily intakes in Santiago, Chile. Nutr 20(2):205–212
FAO/LATINFOODS (2002). Available via DIALOG.
http://www.inta.cl/latinfoodshttp://www.rlc.fao.org/bases/alimento/default.htm. Accessed 25 Jan 2008
Janari M, Cassens R (1991) Mitochondrial activity and beef muscle color stability. J Food Sci 56:1476–1479
Renerre M (1982) Effects of age and slaughter weight on the colour of beef (Friesian and Charolais breeds). Sci des Alim 2:17–30
Renerre M (1986) Influence de facteurs biologiques et technologiques sur la couleur de la viande bovine. Bull Tech 65:41–45
Renerre M, Valin C (1979) Influence de l´age sur les caracteristiques de la coleur des viandes bovines de race Limousine. Tech Agric 28:319–332
OMS/INCAP (2006). Available via DIALOG. http://www.tabladealimentos.net/tca/TablaAlimentos/consideracioes1.html. Accessed 25 Jan 2008
Hallberg L, Bjorn-Rasmussen E, Howard L, Rossander L (1979) Dietary heme iron absorption. A discussion of possible mechanisms for the absorption-promoting affect of meat and for the regulation of iron absorption. Scan J Gastroent 14:769–779
Hallberg L, Rossanderhulthen L, Brune M, Gleerup A (1993) Inhibition of heme-iron absorption in man by calcium. Br J Nutr 69:533–540
Lynch S, Dassenko S, Mork T, Beard J, Cook J (1985) Soy protein products and heme iron absorption in humans. Am J Clin Nutr 41:13–20
Lynch S (1997) Interaction of iron with other nutrients. Nutr Rev 55:102–110
Miret S, Simpson R, Mckie A (2003) Physiology and molecular biology of dietary iron absorption. Annu Rev Nutr 23:283–301
Sandstrom B (2001) Micronutrient interactions: effects on absorption and bioavailability. Br J Nutr 85(Suppl 2):S181–S185
Livingston D, Brown W (1981) The chemistry of myoglobin and its reactions. Food Technol 25:244–252
Martínez-Torres C, Leets I, Taylor P, Ramírez J, Del Valle M, Layrisse M (1986) Heme, ferritin and vegetables iron absorption in humans from denatured of heme iron during the cooking of beef. J Nutr 116:1720–1725
Theil E (2004) Iron, ferritin, and nutrition. Annu Rev Nutr 24:327–343
Pollycove M, Mortimer R (1961) The quantitative determination of iron kinetics and hemoglobin synthesis in human subjects. J Clin Invest 40:753–780
Underwood E (1977) Trace elements in human and animal nutrition, 4th edn. Academic, New York
CV, MO, MSM, and FP conceptualized and designed the study; CV and FP collected and analyzed the data; CV, DLdR, MO, MSM, and FP interpreted the data; CV, DLdR, and FP drafted and revised the manuscript. The author gratefully acknowledges the technical assistance of Maria Angelica Letelier. This work was supported by Fondo Nacional de Desarrollo Científico y Tecnologico (Fondecyt) No. 1061060. All authors approved the final version of the manuscript. All authors declare no general, financial, or institutional competing interests.
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Valenzuela, C., López de Romaña, D., Olivares, M. et al. Total Iron and Heme Iron Content and their Distribution in Beef Meat and Viscera. Biol Trace Elem Res 132, 103–111 (2009). https://doi.org/10.1007/s12011-009-8400-3
- Heme iron