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Biological Trace Element Research

, Volume 130, Issue 3, pp 229–240 | Cite as

Relevance of Non-ceruloplasmin Copper to Oxidative Stress in Patients with Hepatocellular Carcinoma

  • Arumugam GeethaEmail author
  • Panneerselvam Saranya
  • Sam Annie Jeyachristy
  • Rajagopal Surendran
  • Arunachalam Sundaram
Article

Abstract

Altered copper homeostasis and oxidative stress have been observed in patients with hepatocellular carcinoma. Non-ceruloplasmin copper, the free form, is a potent pro-oxidant than the protein bound copper. The aim of the present study was to evaluate which form of copper can be correlated with the oxidative stress in the circulation and in the malignant liver tissues of hepatocellular carcinoma patients. Hepatocellular carcinoma patients (grades II and III, n = 18) were enrolled in this study. Serum levels of total, free and bound copper, ceruloplasmin, iron, iron-binding capacity, lipid peroxidation products, and enzymatic and non-enzymatic antioxidants were quantified in serum and in malignant liver tissues and compared with those of normal samples (n = 20). A significant positive correlation between the serum non-ceruloplasmin copper and lipid peroxidation products and negative correlation with antioxidants were observed in hepatocellular carcinoma patients. In liver tissue, glutathione peroxidase, superoxide dismutase, and catalase activity were significantly decreased with concomitant elevation in oxidative stress markers. Our experiment revealed that the elevation in non-ceruloplasmin copper has high relevance with the oxidative stress than the bound copper.

Keywords

Hepatocellular carcinoma Ceruloplasmin Non-ceruloplasmin copper Oxidative stress Antioxidants 

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Copyright information

© Humana Press Inc. 2009

Authors and Affiliations

  • Arumugam Geetha
    • 1
    Email author
  • Panneerselvam Saranya
    • 1
  • Sam Annie Jeyachristy
    • 1
  • Rajagopal Surendran
    • 2
  • Arunachalam Sundaram
    • 3
  1. 1.Department of BiochemistryBharathi Women’s CollegeChennaiIndia
  2. 2.Department of Surgical Gastroenterology and ProctologyStanley Medical College and HospitalChennaiIndia
  3. 3.Department of PathologyStanley Medical College and HospitalChennaiIndia

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